Effects of Adsorbent on Growth Performance, Organ Indexes, Serum Biochemical Indexes and Antioxidant Capacity of Broilers Fed Diets Contaminated with Aflatoxin B1 and T-2 Toxin

TIAN Sha; SUN Xindong; SONG Zehe; WU Yuanyuan; CHEN Yuguang; HE Xi

doi:10.3969/j.issn.1006-267x.2020.07.024

2020 , Vol. 32 >Issue 7: 3143 - 3153

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.024

Effects of Adsorbent on Growth Performance, Organ Indexes, Serum Biochemical Indexes and Antioxidant Capacity of Broilers Fed Diets Contaminated with Aflatoxin B₁ and T-2 Toxin

TIAN Sha ,
SUN Xindong ,
SONG Zehe ,
WU Yuanyuan ,
CHEN Yuguang ,
HE Xi

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1. College of Animal Science and Technology, Hunan Engineering Research Center of Poultry Production Safety, Hunan Co-Innovation Center of Animal Production Safety, Engineering Research Center of Feed Safety and Efficient Use of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
2. Trouw Nutrition Technology Co., Ltd., Beijing 100089, China

Received date: 2020-02-03

Online published: 2020-07-15

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Abstract

This experiment was conducted to investigate the effects of combination of aflatoxin B₁ (AFB₁) and T-2 toxin on growth performance, organ indexes, serum antioxidant indexes and biochemical indexes of broilers, and to evaluate the detoxification effects of adsorbent. Four hundred and forty-eight one-day-old healthy Arbor Acres (AA) broilers were randomly divided into 4 groups with 8 replicates per group and 14 broilers per replicate (males and females in half) by a 2×2 two factor completely randomized experiment design. Broilers in control group were fed a basal diet, that in group I were fed the basal diets supplemented with 0.25% adsorbent, that in group II were fed the basal diets supplemented with 0.2 mg/kg AFB₁+1.0 mg/kg T-2 toxin, and the others in group III were fed the basal diets supplemented with 0.25% adsorbent+0.2 mg/kg AFB₁+1.0 mg/kg T-2 toxin. Control group and group I were blank groups, and group II and group III were mycotoxin groups. The experiment lasted for 42 days. The results showed as follows:compared with blank groups, 1) supplemented with mycotoxin significantly increased the feed gain ratio(F/G)of broilers at 1 to 21 days of age (P<0.05), and significantly decreased final body weight and average daily gain (ADG) at 22 to 42 days of age (P<0.05); supplemented with adsorbent significantly increased final body weight and average daily feed intake (ADFI) at 22 to 42 days of age (P<0.05). 2) Supplemented with mycotoxin significantly increased spleen index of broilers at 21 days of age (P<0.05), and significantly decreased bursa of Fabricius index at 42 days of age (P<0.05); supplemented with adsorbent alleviated bursa of Fabricius atrophy at 42 days of age, but the effect was not significant (P>0.05). 3) Supplemented with mycotoxin significantly decreased serum catalase (CAT) activity of broilers at 21 days of age and serum total protein (TP) content at 42 days of age (P<0.05), and significantly increased serum alanine aminotransferase (ALT) activity at 21 days of age (P<0.05); supplemented with adsorbent had no significant effects on improvement of serum antioxidant capacity (P>0.05), significantly decreased serum alkaline phosphatase (ALP) activity at 21 days of age (P<0.05), and significantly increased serum albumin (ALB) content at 42 days of age (P<0.05). In conclusion, adsorbent can effectively alleviate the negative effects of AFB₁ and T-2 toxin on growth performance, organ indexes and serum biochemical indexes of broilers, so it can be added to broiler's diets as an effective detoxification agent.

Key words： broiler; aflatoxin B₁; T-2 toxin; adsorbent; growth performance; antioxidant index; serum biochemical index

Cite this article

TIAN Sha , SUN Xindong , SONG Zehe , WU Yuanyuan , CHEN Yuguang , HE Xi . Effects of Adsorbent on Growth Performance, Organ Indexes, Serum Biochemical Indexes and Antioxidant Capacity of Broilers Fed Diets Contaminated with Aflatoxin B₁ and T-2 Toxin[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3143 -3153 . DOI: 10.3969/j.issn.1006-267x.2020.07.024

References

[1] 柳永振,朱凤华,宁雪娇,等.霉菌毒素联合毒性研究进展[J].中国饲料,2013(3):33-36.
[2] 刘杉杉,孙伟,杨慧,等.饲料中黄曲霉毒素对家禽的危害及防治[J].农业与技术,2019,39(21):139-140.
[3] 符小杏,杨信,李强,等.饲料中T2毒素对家禽的危害及其脱毒研究进展[J].饲料研究,2013(10):21-23.
[4] 胡文娟.复合吸附剂对黄曲霉毒素B₁和脱氧雪腐镰刀菌烯醇联合毒性的脱毒效果研究[D].硕士学位论文.合肥:安徽农业大学,2016.
[5] BATTACONE G,NUDDA A,PULINA G.Effects of ochratoxin a on livestock production[J].Toxins,2010,2(7):1796-1824.

[6] SPEIJERS G J A,SPEIJERS M H M.Combined toxic effects of mycotoxins[J].Toxicology Letters,2004,153(1):91-98.

[7] FINK-GREMMELS J.The role of mycotoxins in the health and performance of dairy cows[J].The Veterinary Journal,2008,176(1):84-92.

[8] SWAMY H V L N,SMITH T K,KARROW N A,et al.Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens[J].Poultry Science,2004,83(4):533-543.

[9] JIANG S Z,LI Z,WANG G Y,et al.Effects of Fusarium mycotoxins with yeast cell wall absorbent on hematology,serum biochemistry,and oxidative stress in broiler chickens[J].Journal of Applied Poultry Research,2014,23(2):165-173.

[10] YUNUS A W,RAZZAZI-FAZELI E,BOHM J.Aflatoxin B₁ in affecting broiler's performance,immunity,and gastrointestinal tract:a review of history and contemporary issues[J].Toxins,2011,3(6):566-590.

[11] 左瑞雨,王秋文,常娟,等.AFB₁降解剂对肉鸡生长性能、养分代谢及AFB₁在体内残留的影响[J].中国畜牧兽医,2017,44(02):432-439.
[12] 邓庆庆,刘宁,江青东,等.乳酸菌对饲喂黄曲霉毒素B₁日粮的肉鸡生长性能、养分消化率和屠宰性能的影响[J].中国畜牧兽医,2016,43(5):1194-1200.
[13] 宋洪国.黄曲霉毒素B₁对肉鸭消化道结构和功能、血液生化指标及生产性能的影响研究[D].硕士学位论文.长沙:湖南农业大学,2012:22-25.
[14] 李丽霞,尚书凤.T-2毒素的毒性及其作用机制与代谢研究进展[J].湖北农业科学,2015,54(21):5207-5210.
[15] 黄凯,朱祖贤,朱凤华,等.饲料中T-2毒素的毒性作用研究进展[J].中国饲料,2014(18):9-10,16.
[16] BRAKE J,HAMILTON P B,KITTRELL R S.Effects of the trichothecene mycotoxin diacetoxyscirpenol on feed consumption,body weight,and oral lesions of broiler breeders[J].Poultry Science,2000,79(6):856-863.

[17] 何姝颖.黄曲霉毒素污染玉米及霉菌毒素吸附剂对肉鸡不同阶段生产性能和健康的影响[D].硕士学位论文.雅安:四川农业大学,2015:58-59.
[18] NURYONO,AGUS A,WEDHASTRI S,et al.Adsorption of aflatoxin B₁ in corn on natural zeolite and bentonite[J].Indonesian Journal of Chemistry,2012,12(3):279-286.

[19] 陈光明,刘建军,刘桂兰,等.不同霉菌毒素吸附剂对肉鸡生长性能和血清生化指标的影响[J].饲料研究,2015(19):27-29.
[20] FERLAY J,SHIN H R,BRAY F,et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008[J].International Journal of Cancer,2010,127(12):2893-2917.

[21] 郭铭生,石达友,刘念,等.黄曲霉毒素B₁对肉鸡肝线粒体膜通透性的影响[J].中国家禽,2010,32(5):61-62.
[22] 温子瑜.黄曲霉毒素污染玉米及霉菌毒素吸附剂对肉鸭生产性能和健康的影响[D].硕士学位论文.雅安:四川农业大学,2012.
[23] 黄晓琳.霉菌毒素在肉鸡体内残留及其控制研究[D].硕士学位论文.杭州:浙江工商大学,2012.
[24] 王兆钧,王春维.霉变日粮中添加酵母葡聚糖对肉仔鸡生长性能、器官指数及血清指标的影响[J].饲料工业,2011,32(14):44-48.
[25] 胡贵丽.吸附剂与黄曲霉毒素B₁对白羽肉鸡生长性能、肠道和免疫功能的影响[D].硕士学位论文.长沙:湖南农业大学,2018.
[26] 陈凤鸣,陈佳亿,李颖慧,等.霉菌毒素介导的氧化应激研究进展[J].动物营养学报,2019,31(9):3944-3952.
[27] CHEN J,CHEN K J,YUAN S B,et al.Effects of aflatoxin B₁ on oxidative stress markers and apoptosis of spleens in broilers[J].Toxicology and Industrial Health,2013,32(2):278-284.
[28] FRANKI?R V,NARAT M,LEVART A,et al.Dose-dependent effects of T-2 toxin on performance,lipid peroxidation,and genotoxicity in broiler chickens[J].Poultry Science,2007,86(6):1155-1160.

[29] GIRISH C,SMITH T.Impact of feed-borne mycotoxins on avian cell-mediated and humoral immune responses[J].World Mycotoxin Journal,2008,1(2):105-121.

[30] CHE Z Q,LIU Y L,WANG H R,et al.The protective effects of different mycotoxin adsorbents against blood and liver pathological changes induced by mold-contaminated feed in broilers[J].Asian-Australasian Journal of Animal Sciences,2011,24(2):250-257.
[31] KALEIBAR M T,HELAN J A.A field outbreak of aflatoxicosis with high fatality rate in feedlot calves in Iran[J].Comparative Clinical Pathology,2013,22(6):1155-1163.

[32] MARIN S,RAMOS A J,CANO-SANCHO G,et al.Mycotoxins:occurrence,toxicology,and exposure assessment[J].Food and Chemical Toxicology,2013,60:218-237.
[33] HOU Y J,ZHAO Y Y,XIONG B,et al.Mycotoxin-containing diet causes oxidative stress in the mouse[J].PLoS One,2013,8(3):e60374.
[34] 陈静,周振金,刘乃芝,等.复合型霉菌毒素脱毒剂对海兰褐蛋鸡生产性能、蛋品质及血清生化指标的影响[J].饲料工业,2017,38(9):34-38.
[35] 史艳云,刘兵,熊平文,等.枯草芽孢杆菌对饲喂含黄曲霉毒素B₁日粮蛋鸡产蛋性能和蛋品质及血清生化指标的影响[J].中国畜牧杂志,2015,51(17):69-73.
[36] VAN RENSBURG C J,VAN RENSBURG C E J,VAN RYSSEN J B J,et al.In vitro and in vivo assessment of humic acid as an aflatoxin binder in broiler chickens[J].Poultry Science,2006,85(9):1576-1583.

[37] LI Z,YANG Z B,YANG W R,et al.Effects of feed-borne Fusarium mycotoxins with or without yeast cell wall adsorbent on organ weight,serum biochemistry,and immunological parameters of broiler chickens[J].Poultry Science,2012,91(10):2487-2495.

[38] ZHAO J,SHIRLEY R B,DIBNER J D,et al.Comparison of hydrated sodium calcium aluminosilicate and yeast cell wall on counteracting aflatoxicosis in broiler chicks[J].Poultry Science,2010,89(10):2147-2156.

[39] KATTESH H G,BROWN M E,MASINCUPP F B,et al.Protein-bound and unbound forms of plasma cortisol in piglets after castration at seven or 14 days of age[J].Research in Veterinary Science,1996,61(1):22-25.

[40] 郑爱娟,吴正可,PIRZADO S A,等.丁酸梭菌对肉鸡生长性能和血清生化指标的影响[J].动物营养学报,2019,31(12):5519-5525.

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