Effects of Compound Microecologics on Growth Performance and Rumen Microbiota of Calves in Lactation Period

CHEN Fengmei; CHENG Guangmin; ZHANG Yongcui; GUO Jianqiang; NIU Zhongxiang; HU Shilin

doi:10.3969/j.issn.1006-267x.2020.07.033

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 7: 3234 - 3245

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.033

Effects of Compound Microecologics on Growth Performance and Rumen Microbiota of Calves in Lactation Period

CHEN Fengmei ,
CHENG Guangmin ,
ZHANG Yongcui ,
GUO Jianqiang ,
NIU Zhongxiang ,
HU Shilin

Expand

1. Shandong Research Center for Tech of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China;
2. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2020-02-04

Online published: 2020-07-15

Fold

Abstract

This study was conducted to investigate the effects of compound microecologics on growth performance and rumen microflora of calves in lactation period. Twenty-one 3-day-old healthy Holstein female calves were randomly divided into three groups with 7 calves in each group, and raised in a single column. Calves in the control group (group C) were fed a basal diet, calves in Lactobacillus plantosus+Bacillus subtilis group (group ZK) were fed the basal diet+5 g Lactobacillus plantosus+5 g Bacillus subtilis each calf per day, and calves in Lactobacillus plantosus+Bacillus subtilis+fructoses oligosaccharide group (group ZK) were fed the basal diet+5 g Lactobacillus plantosus+5 g Bacillus subtilis+5 g fructoses oligosaccharide each calf per day. Calves in ZK group and ZKT group were infused compound microecologics with bottle after feeding basal diets 1 hour, which were dissolved in warm water. The pre-experimental period lasted for 7 days, and the experimental period lasted for 50 days. The results showed as follows:there were no significant differences in average daily gain and average daily feed intake among all groups (P>0.05). The analysis of microbial community Alpha diversity showed that there were no significant differences in observed species number, Chao1 index, ACE index, Shannon index and Simpson index among all groups (P>0.05); the analysis of Beta diversity showed that the ZKT group had better clustering analysis based on unweighted unifrac distance principal component analysis, and the relative dispersion between the group C and group ZK indicated that the community structure between the groups had great difference. The UPGMA clustering analysis was performed by unweighted unifrac distance matrix, the clustering tree could not effectively distinguish group C, group ZK had better clustering, and group ZKT had the best clustering, it indicated that the addition of compound microecologics could affected the rumen microflora of calves. Based on the analysis of bacterial colony composition of rumen contents, it was found that the dominant phylum in the calf gastric juice were Bacteroides, Firmicutes, Actinomycetes et al, and the dominant genus were Olsenella, unidentified_Prevotellaceae and Acetitomaculum et al. Tax4Fun functional prediction showed that the abundances of gene expression of group ZK in three primary functional layers which included cell process, environmental information processing and unidentified functions were increased than those of group C, and the abundances of gene expression of group ZK in five second functional layers which included genetic information processing, infectious diseases, amino acid metabolism, metabolism of other amino acids and carbohydrate metabolism were increased than those of group C and group ZKT. In conclusion, the compound microecologics have no significant effects on the growth performance and richness and diversity of rumen microbiota of calves in lactation. However, the rumen microbiota of calves before weaning of group ZKT obvious change, and the ZK group is better than the ZKT group in function prediction.

Key words： compound microecologics; high throughput sequencing; calves in lactation; growth performance; rumen microbiota; function prediction

Cite this article

CHEN Fengmei , CHENG Guangmin , ZHANG Yongcui , GUO Jianqiang , NIU Zhongxiang , HU Shilin . Effects of Compound Microecologics on Growth Performance and Rumen Microbiota of Calves in Lactation Period[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3234 -3245 . DOI: 10.3969/j.issn.1006-267x.2020.07.033

References

[1] WELKIE D G,STEVENSO D M,WEIMER P J.PARISA analysis of ruminal bacterial community dynamics in lactating dairy cows during the feeding cycle[J].Anaerobe,2010,16(2):94-100.

[2] JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The ISME Journal,2013,7(6):1069-1079.

[3] SHANKS O C,KELTY A,ARCHIBEQUE S,et al.Community structures of fecal bacteria in cattle from different animal feeding operations[J].Applied and Environmental Microbiology,2011,77(9):2992-3001.

[4] REY M,ENJALBERT F,COMBES S,et al.Establishment of ruminal bacterial community in dairy calves from birth to weaning is sequential[J].Journal of Applied Microbiology,2014,116(2):245-257.

[5] MULLINS C R,MAMEDOVA L K,CARPENTER A J,et al.Analysis of rumen microbial populations in lactating dairy cattle fed diets varying in carbohydrate profiles and Saccharomyces cerevisiae fermentation product[J].Journal of Dairy Science,2013,96(9):5872-5881.

[6] ABD EL-TAWAB M M,YOUSSEF I M,BAKR H A,et al.Role of probiotics in nutrition and health of small ruminants[J].Polish Journal of Veterinary Sciences,2016,19(4):893-906.

[7] 符运勤,刁其玉,屠焰,等.不同组合益生菌对0-8周龄犊牛生长性能及血清生化指标的影响[J].动物营养学报,2012,24(4):753-761.
[8] 张海涛.纳豆枯草芽孢杆菌对犊牛生长发育以及瘤胃组织形态学发育的影响[D].硕士学位论文.北京:中国农业科学院,2009.
[9] KAWAKAMI S I,YAMADA T,NAKANISHI N,et al.Feeding of lactic acid bacteria and yeast affects fecal flora of holstein calves[J].Journal of Animal and Veterinary Advances,2012,10(3):269-271.
[10] 董晓丽.益生菌的筛选鉴定及其对断奶仔猪、犊牛生长和消化道微生物的影响[D].博士学位论文.北京:中国农业科学院,2013.
[11] FODITSCH C,VAN VLECK PEREIRAR,GANDA E K,et al.Oral administration of Faecalibacterium prausnitzii decreased the incidence of severe diarrhea and related mortality rate and increased weight gain in preweaned dairy heifers[J].PLoS One,2015,10(12):e0145485.
[12] BAYATKOUHSAR J,TAHMASEBI A M,NASERIAN A A,et al.Effects of supplementation of lactic acid bacteria on growth performance,blood metabolites and fecal coliform and lactobacilli of young dairy calves[J].Animal Feed Science and Technology,2013,186(1/2):1-11.
[13] RIDDELL J B,GALLEGOS A J,HARMON D L,et al.Addition of a Bacillus based probiotic to the diet of preruminant calves:infuence on growth,health,and blood parameters[J].Journal of Applied Research in Veterinary Medicine,2008,(1):78-85.
[14] 聂炼.WB800N-CC31工程菌和屎肠球菌及其复合菌对犊牛生长性能及肠道菌群的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2019.
[15] UYENO Y,SHIGEMORI S,SHIMOSATO T.Effect of probiotics/prebiotics on cattle health and productivity[J].Microbes and Environments,2015,30(2):126-132.

[16] KIDO K,TEJIMA S,NAGAYAMA H,et al.Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture[J].Animal Science Journal,2016,87(5):661-665.

[17] 李文茜.丁酸梭菌对犊牛及育成牛生长性能、血液指标和瘤胃发酵的影响[D].硕士学位论文.哈尔滨:东北农业大学,2019.
[18] DIAS J,MARCONDES M I,DE SOUZA S M,et al.Bacterial community dynamics across the gastrointestinal tracts of dairy calves during preweaning development[J].Applied and Environmental Microbiology,2018,84(9):e02675-17.
[19] CHAUCHEYRAS-DURAND F,OSSA F.Review:the rumen microbiome:composition,abundance,diversity,and new investigative tools[J].Professional Animal Scientist,2014,30(1):1-12.

[20] WANG L Z,XU Q,KONG F L,et al.Exploring the goat rumen microbiome from seven days to two years[J].PLoS One,2016,11(5):e0154354.
[21] 李晓斌,赵国栋,邱昊日,等.L-瓜氨酸对小鼠体重及肠道菌群多样性的影响[J].动物营养学报,2019,31(9):4235-4241.
[22] SASSON G,BEN-SHABAT S K,SEROUSSI E,et al.Heritable bovine rumen bacteria are phylogenetically related and correlated with the cow's capacity to harvest energy from its feed[J].mBio,2017,8(4):e00703-17.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References