The purpose of this experiment was to study the effects of compound bacteria culture and yeast β-glucan on the growth performance and immune function of mutton sheep. In the experiment, 105 healthy weaned male lambs aged 50 days were randomly divided into 3 groups. The control group was fed a basal total mixed ration (TMR) diet, while the test group I was fed diets supplemented with a compound bacteria culture (50 g/kg in the early stage and 70 g/kg in the middle and late stages) and the test group Ⅱ was fed the TMR diet added with yeast β-glucan (75 mg/kg). The test lasted for 137 days including a 7-d preliminary period and a 130-d test period. The growth performance indicators were evaluated by lamb weights at days 1, 30, 60, 90, and 130, and the blood samples were collected at days 1, 30, 60, and 90 to determine the immune parameters. The results showed as follows:1) compared with the control group, the average body weights in test groups Ⅰ and Ⅱ increased by 10.02% and 5.05% (P<0.05), respectively; the dressing percentages increased by 1.29%and 1.10% (P>0.05); the feed/gain decreased by 10.33% (P<0.05) and 4.21% (P>0.05). 2) Compared with the control group, the serum acid phosphatase activity and lysozyme content of test group Ⅰ significantly increased (P<0.05); the contents of immune globulin G, immune globulin M, CD₃⁺, interleukin-lβ, interleukin-2, interleukin-6, interferon-γ, tumor necrosis factor-α and CD₄⁺/CD₈⁺ in the serum of test group Ⅰ significantly enhanced (P<0.05), and the contents of immune globulin G, immune globulin M, interleukin-lβ, of test group Ⅱ significantly significantly increased (P<0.05). This results indicate that both the compound bacteria culture and yeast β-glucan can significantly increase the average body weight of lambs. Moreover, the compound bacteria culture not only markedly improve the specific and non-specific immunity but also reduce the feed/gain; yeast β-glucan dramatically increase the specific immunity of mutton sheep.