Isolation and Identification of Lactobacillus johnsonii Strain ZJBF004 from Alopex lagopus and Its Probiotic Potential Evaluation in Vitro

  • CAI Xiheng ,
  • LIU Hanlu ,
  • ZHANG Ruchun ,
  • SI Huazhe ,
  • WANG Jinming ,
  • ZHANG Xinyu ,
  • LIU Chaonan ,
  • LI Guangyu
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  • 1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    2. Jilin Agricultural University, Changchun 130118, China

Received date: 2020-01-15

  Online published: 2020-07-15

Abstract

In order to obtain a canine source of lactic acid bacteria and evaluate its probiotic potential, in this study, the probiotic characteristics of strain ZJBF004 were evaluated by measuring its growth curve, acid production capacity, tolerance to low pH and high concentration of bile salt, viability in vitro simulated gastrointestinal solution, antibacterial capacity, antibiotic resistance, adhesion to primary Alopex lagopus intestinal epithelial cells and antioxidant activity. The results showed that a dominant lactic acid bacteria was isolated from Alopex lagopus's intestinal tract by solid Lactobacillus culture medium and it was named strain ZJBF004. The strain ZJBF004 was identified as Lactobacillus johnsonii by the analysis of colony morphology, physiological and biochemical characteristics and 16S rRNA sequence analysis. The strain ZJBF004 had strong acid-producing ability and was able to enter logarithmic growth phase rapidly in 37℃; the strain ZJBF004 could maintain good growth in the medium containing with pH 5.0, 70% bacteria were survived in tolerant to 3 g/L bile salt in 6 hours, and 90% bacteria were survived in simulated gastric and intestinal juices in 6 hours; the antimicrobial effect of strain ZJBF004 on pathogens which Escherichia coli, Salmonella and Staphylococcus aureus was good. Moreover, the adhesion ability of strain ZJBF004 to Alopex lagopus intestinal primary epithelial cells was (18.67±3.12) bacterias/cell, and the 1,1-diphenyl-2-trinitrophenyl hydrazine (DPPH) radical scavenge rate was 28.50%, which showed the strain ZJBF004 had a certain antioxidant activity. In conclusion, the newly isolated Lactobacillus johnsonii strain ZJBF004 has good beneficial effects in vitro and can be further studied as candidate probiotics for feeding canines.

Cite this article

CAI Xiheng , LIU Hanlu , ZHANG Ruchun , SI Huazhe , WANG Jinming , ZHANG Xinyu , LIU Chaonan , LI Guangyu . Isolation and Identification of Lactobacillus johnsonii Strain ZJBF004 from Alopex lagopus and Its Probiotic Potential Evaluation in Vitro[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3313 -3323 . DOI: 10.3969/j.issn.1006-267x.2020.07.041

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