Effects of Lipopolysaccharide on Casein Gene Expression in Rat Mammary Epithelial Cells

  • TIAN Qing ,
  • SPITZER A J ,
  • ZHAO Fengqi
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  • 1. Jiangsu Food&Pharmaceutical Science College, Huaian 223003, China;
    2. The University of Vermont, Burlington 05405, USA

Received date: 2020-02-19

  Online published: 2020-07-15

Abstract

The purpose of this study was to investigate the effects of lipopolysaccharide (LPS) on major casein genes[αs1-casein (CSN1S1), β-casein (CSN2) and α-lactalbumin (α-LA)] expression in mammary epithelial cells. Single factor test design was adopted, and the effects of LPS at doges of 0, 100, 500, 1 000, 5 000 and 25 000 ng/mL on the casein genes (CSN1S1, CSN2 and α-LA), glucose transporters 1 (GLUT1), glucose transporters 8 (GLUT8) and interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) genes expression in HC11 cells induced by prolactin at 3 and 34 h using RT-PCR. The results showed as follows:the LPS inhibited the expression of CSN2 gene both in 3 and 24 h. LPS significantly inhibited the α-LA gene expression in 24 h (P<0.05), but the doges of 500 to 1 000 ng/mL LPS significantly increased its expression in 3 h in HC11 cells (P<0.05). The LPS at the doges of 500 to 25 000 ng/mL in 3 h and LPS at all doges in 24 h significantly increased the expression of CSN1S1 gene (P<0.05). LPS significantly increased TNF-α gene expression (P<0.05). The doges of 1 000 ng/mL LPS in 3 h and 100 to 25 000 ng/mL LPS in 24 h increased the expression of IL-6 gene significantly (P<0.05), and IL-1β gene expression was significantly increased at the doges of 500 to 25 000 ng/mL LPS in 3 h (P<0.05). The overall trend was that the gene expression of all three cytokines (IL-6, IL-1β and TNF-α)in 24 h was higher than that in 3 h. The genes expression of GLUT1 increased significantly at the doges more than 5 000 ng/mL LPS, but GLUT8 gene expression inhibited significantly (P<0.05). In conclusion, LPS has different effects on the expression of different casein genes in mammary epithelial cells. LPS can inhibit the expression of CSN2 and α-LA genes, but can enhance the expression of CSN1S1 gene.

Cite this article

TIAN Qing , SPITZER A J , ZHAO Fengqi . Effects of Lipopolysaccharide on Casein Gene Expression in Rat Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3365 -3373 . DOI: 10.3969/j.issn.1006-267x.2020.07.046

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