Screen of Two Bacterial Strains of Bacillus Isolated from Goat and Their Effects on Intestinal Biochemical Indexes and Intestinal Microflora of Mice

  • ZHANG Nanchi ,
  • WANG Li ,
  • WEI Yong
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  • 1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China;
    2. Animal Genetics and Breeding Key Laboratory of Sichuan Province, Animal Science Academy of Sichuan Province, Chengdu 610066, China

Received date: 2020-02-11

  Online published: 2020-07-15

Abstract

The purpose of this study was to screen bacterial strains with antibacterial activity from goat droppings, and explore their effects on the intestinal biochemical indexes and intestinal microflora of mice. In this study, the strains with antibacterial activity were screened from the goat droppings using the Oxford cup method and named bacterial strains fsznc-06 and fsznc-09, respectively. Sixty healthy mice were randomly divided into 5 groups with 3 replicates per group and 4 mice per replicate. JD1 and JD2 groups were administered orally with bacterial strain fsznc-06 with concentrations of 1×108 and 1×1010 CFU/mL, respectively; DX1 and DX2 groups were administered orally with bacterial strain fsznc-09 with concentrations of 1×108 and 1×1010 CFU/mL, respectively; the control group (DZ group) was administered orally with 0.9% sodium chloride solution. Every 2 days administered orally once, the dosage was 0.3 mL per mouse. After feeding 28 days, the activities of total superoxide dismutase (T-SOD),alkaline phosphatase (AKP) and lysozyme (LZM) in small intestine tissue were determined, and the contents of small intestine were taken for microbial sequencing and diversity analysis. The results showed that two bacterial strains with antibacterial activity were screened in this experiment, they were identified and named as Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09. Compared with the DZ group, the activities of T-SOD, AKP and LZM in small intestine tissue of JD1 and DX1 groups were significantly increased (P<0.05 or P<0.01), the small intestine tissue T-SOD activity of JD2 group was significantly increased (P<0.01), and the small intestine tissue AKP activity in DX2 group was significantly increased (P<0.01). The diversities of intestinal microflora in small intestine of JD1, JD2, DX1 and DX2 groups were higher than those of DZ group. The dominant species in JD1, JD2, DX1, DX2 and DZ groups were Lactobacillus johnsonii (53.54%), Lactobacillus reuteri (44.56%), Lactobacillus johnsonii (45.21%), Lactobacillus johnsonii (41.38%), Lactobacillus johnsonii (44.51%), respectively. The intestinal microflora of JD1 and JD2 groups mainly improved the functions of genetic information processing, and the intestinal microflora of DX1 and DX2 groups mainly improved the functions of environmental information processing, metabolism and organismal systems. In conclusion, Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 can improve the activities of T-SOD, AKP and LZM in small intestine tissue, and increase the diversity of small intestinal microflora and the relative abundance of beneficial microflora of mice.

Cite this article

ZHANG Nanchi , WANG Li , WEI Yong . Screen of Two Bacterial Strains of Bacillus Isolated from Goat and Their Effects on Intestinal Biochemical Indexes and Intestinal Microflora of Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3374 -3385 . DOI: 10.3969/j.issn.1006-267x.2020.07.047

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