Rumen Stability of Rumen-Protected Glucose and Its Effects of Different Doses on Rumen Degradation Characteristics of Oat Hay

WANG Yapin; ZHANG Fan; HUA Dengke; JIANG Linshu; XIONG Benhai

doi:10.3969/j.issn.1006-267x.2020.07.053

2020 , Vol. 32 >Issue 7: 3428 - 3438

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.053

Rumen Stability of Rumen-Protected Glucose and Its Effects of Different Doses on Rumen Degradation Characteristics of Oat Hay

WANG Yapin ,
ZHANG Fan ,
HUA Dengke ,
JIANG Linshu ,
XIONG Benhai

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, China;
2. Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China

Received date: 2020-01-13

Online published: 2020-07-15

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Abstract

The purpose of this study was to evaluate the rumen stability of rumen protected glucose (RPG) and its effects of different doses on the rumen degradation characteristics of oat hay by using the nylon-bag technique, so as to provide theoretical reference for scientific use of RPG to effectively alleviate negative energy balance (NEB). The experiment was divided into two parts. In experiment Ⅰ, four Chinese Holstein cows with permanent ruminal cannulas were selected to evaluate the stability of RPG in the rumen and determine the degradation rate of rumen by measuring the residual amount of glucose remaining in the RPG at different time points, so as to provide theoretical support for subsequent study. In experiment Ⅱ, six Chinese Holstein cows with permanent ruminal cannulas were used and randomly divided into 3 groups with 2 cows per group. Cows in control group were fed a basal diet, and those in 200 and 350 g groups were fed the basal diet added with 200 and 350 g/(head·d) RPG, respectively, and the RPG were fed mixing concentrate. The pre-test lasted for 7 d and the test lasted for 3 d. The dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF) and crude protein (CP) ruminal degradability and degradation parameters of oat hay retained in the rumen for 2, 6, 12, 24, 36, 48 and 72 h were determined to evaluate the effects of different doses of RPG on the degradation characteristics of oat hay. The results showed as follows:1) the effective degradation rate of RPG in the rumen was 45.97%. 2) At different time points (07:00, 14:00 and 21:00), rumen fluid pH of the control group was significantly higher than that in the 350 g group (P<0.05), and rumen fluid ammonia nitrogen (NH₃-N) concentration of the 200 g group was significantly higher than that of the other groups (P<0.05), but there were no significant difference in each volatile fatty acid (expect acetate and isovalerate at 14:00) and total volatile fatty acid concentration (P>0.05). 3) The effective degradation rate of DM for oat hay was not significant among groups (P>0.05); the lowest effective degradation rate of NDF for oat hay was found in 350 g group, which was significantly different from the 200 g group and the control group (P<0.05), at the same time, the effective degradation rate of ADF for oat hay also had a similar change pattern; there was no significant difference in the effective degradation rate of CP for oat hay among groups (P>0.05). In summary, the rumen passage rate of RPG is 54.03%, and the addition of 200 g/(head·d) RPG to the diet can alleviate NEB and regulate rumen fermentation while ensuring that the rumen degradation characteristics of oat hay are not significantly affected.

Key words： energy negative equilibrium; rumen protected glucose; oat hay; rumen degradation rate; fermentation characteristics; nylon-bag technique

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WANG Yapin , ZHANG Fan , HUA Dengke , JIANG Linshu , XIONG Benhai . Rumen Stability of Rumen-Protected Glucose and Its Effects of Different Doses on Rumen Degradation Characteristics of Oat Hay[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3428 -3438 . DOI: 10.3969/j.issn.1006-267x.2020.07.053

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