Expression Analysis of Key Genes Associated with Fatty Acid Synthesis at Different Development Stages of Tenebrio molitor

  • WU Lingli ,
  • ZHENG Tianxiang ,
  • WANG Shengyin ,
  • ZHANG Dayu
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  • 1. School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
    2. School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China

Received date: 2020-01-12

  Online published: 2020-07-15

Abstract

The purpose of this experiment was to study gene expression profile at different development stages of Tenebrio molitor (T. molitor), and to analyze the characteristics and expression features of key genes related to fatty acid synthesis pathways. Illumina HiSeqTM 2000 high-throughput sequencing platform was used to sequence transcriptomes of T. molitor individuals at different developmental stages, and the sequencing results were uploaded to the Uniprot and KEGG databases for analysis. Quantitative PCR was used to detect the expression of key genes related to the fatty acid synthesis of T. molitor at different developmental stages. The results showed that totally 156 761 transcripts and 104 937 unigenes were obtained, and 32 806 unigenes were successfully annotated by compared with Uniprot database, and 14 acetyl-CoA carboxylase (ACC), 75 fatty acid synthetase (FAS), 101 fatty acyl-CoA reductase (FAR) and 75 fatty acid desaturase (FAD) genes were identified. A total of 44 586 unigenes were successfully matched to 331 metabolic pathways through the KEGG database. Quantitative analysis showed that the relative expression levels of ACC and FAS genes in the male adult stage was significantly higher than those in other developmental stages (P<0.05), while the relative expression level of FAD gene in the female adult stage was significantly higher than that in other developmental stages (P<0.05), it showed that the ACC, FAS and FAD genes may be played important roles at adult stage of T. molitor. FAR gene was expressed at all developmental stages, it showed that the FAR gene was involved in fatty acid synthesis in each developmental stage. The results of this study indicate that fatty acid synthesis of T. molitor is related to the developmental stage, the metabolic intensity and the relative expression of fatty acid synthesis-related genes of young larvae are high. The expression levels of ACC, FAS, FAR and FAD genes of T. molitor at different developmental stages are age-specific, revealing that they are related to specific physiological functions of each age.

Cite this article

WU Lingli , ZHENG Tianxiang , WANG Shengyin , ZHANG Dayu . Expression Analysis of Key Genes Associated with Fatty Acid Synthesis at Different Development Stages of Tenebrio molitor[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3439 -3450 . DOI: 10.3969/j.issn.1006-267x.2020.07.054

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