Effects of Glucose Oxidase on Growth Performance, Serum Biochemical and Antioxidant Indexes, Intestinal Morphology and Microbial Diversity of 1 to 6-Week-Old Linwu Ducks

WANG Qianguang; LI Chuang; HUANG Xuan; ZHANG Xu; JIANG Guitao

doi:10.3969/j.issn.1006-267x.2020.08.019

2020 , Vol. 32 >Issue 8: 3605 - 3614

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.019

Effects of Glucose Oxidase on Growth Performance, Serum Biochemical and Antioxidant Indexes, Intestinal Morphology and Microbial Diversity of 1 to 6-Week-Old Linwu Ducks

WANG Qianguang ,
LI Chuang ,
HUANG Xuan ,
ZHANG Xu ,
JIANG Guitao

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1. Changde Vocational Technical College, Changde 415000, China;
2. Hunan Institute of Animal Science and Veterinary Medicine, Changsha 410131, China;
3. Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China

Received date: 2020-02-28

Online published: 2020-08-13

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Abstract

This experiment was conducted to investigate the effects of dietary glucose oxidase (GOD) on growth performance, serum biochemical and antioxidant indexes, intestinal morphology and microbial diversity of 1 to 6-week-old Linwu ducks, and to determine the GOD suitable supplement level based on quadratic regression model. Four hundred and eighty healthy 7-day-old Linwu ducks were randomly assigned to 5 groups with 6 replicates per group and 16 ducks per replicate. Ducks in the control group were fed a basal diet, and the others in experimental groups were fed basal diets supplemented with 10, 20, 30 and 40 g/t GOD (activity was 10 000 U/g), respectively. The trail period lasted for 35 days. The results showed as follows:1) there were no significant differences in final weight, average daily gain, average daily feed intake and feed to gain ratio among all groups (P>0.05). 2) There were no significant differences in total protein, albumin contents and superoxide dismutase, glutathione peroxidase activities in serum among all groups (P>0.05). The serum glucose content of groups B and C was significantly higher than that of the control group (P<0.05), the serum urea nitrogen content of groups A, B, C and D was significantly lower than that of the control group (P<0.05), the serum glutathione content of group D was significantly higher than that of other groups (P<0.05), and the serum malondialdehyde content of group C was significantly lower than that of other groups (P<0.05). 3) The duodenal intestinal wall thickness of groups B and D was significantly lower than that of group A and control group (P<0.05), the jejunal villus height/crypt depth of group C was significantly higher than that of group D (P<0.05), the ileum villus height of group A was significantly higher than that of group C (P<0.05), and the ileum intestinal wall thickness of groups B, C and D was significantly lower than that of group A and control group (P<0.05). 4) The richness index of ileum chyme microorganism of groups A and B was significantly higher than that of the control group (P<0.05). The evenness index, Shannon index and Simpson index of cecum chyme microorganism of group A were significantly higher than those of the control group (P<0.05), and the richness index of cecum chyme microorganism of group C was significantly higher than that of the control group (P<0.05). In conclusion, the diet of 1 to 6-week-old Linwu ducks supplemented with proper GOD can improve the serum biochemical indexes, enhance the body antioxidation ability, and improve the microbial diversity of ileum and cecum in different degree. Based on quadratic regression models, the dietary GOD suitable supplement levels of 1 to 6-week-old Linwu ducks to gain maximum serum glucose content, minimum serum urea nitrogen content and maximum richness index of ileum chyme microorganism are 24.04, 29.40 and 19.70 g/t.

Key words： glucose oxidase; Linwu ducks; growth performance; serum biochemical indexes; antioxidant indexes; intestinal morphology; microbial diversity

Cite this article

WANG Qianguang , LI Chuang , HUANG Xuan , ZHANG Xu , JIANG Guitao . Effects of Glucose Oxidase on Growth Performance, Serum Biochemical and Antioxidant Indexes, Intestinal Morphology and Microbial Diversity of 1 to 6-Week-Old Linwu Ducks[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3605 -3614 . DOI: 10.3969/j.issn.1006-267x.2020.08.019

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