Effects of Black Soldier Fly Larvae Meal on Growth Performance, Serum Biochemical Indexes and Antioxidant Capacity of Layer Chickens

WANG Guiying; WANG Kuiming; LI Lusheng; LIU Cheng; GAO Pengxiang; DONG Bingqiang

doi:10.3969/j.issn.1006-267x.2020.08.020

2020 , Vol. 32 >Issue 8: 3615 - 3623

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.020

Effects of Black Soldier Fly Larvae Meal on Growth Performance, Serum Biochemical Indexes and Antioxidant Capacity of Layer Chickens

WANG Guiying ,
WANG Kuiming ,
LI Lusheng ,
LIU Cheng ,
GAO Pengxiang ,
DONG Bingqiang

Expand

1. College of Agriculture, Liaocheng University, Liaocheng 252000, China;
2. College of Life Science, Liaocheng University, Liaocheng 252000, China

Received date: 2020-02-11

Online published: 2020-08-13

Fold

Abstract

This experiment was conducted to study the feasibility of using black soldier fly larva meal in the diet of layer chickens. A total of 480 healthy 1-day-old Hailan brown layer chickens were randomly divided into 4 groups with 6 replicates in each group and 20 replicates in each group. Layer chickens in G0, G3, G6 and G9 groups were fed diets contained 0, 3%, 6% and 9% of the black soldier fly larval meal (made by 15 instar larvae that fed with kitchen waste), respectively. The 4 diets were isonitrogenous and isoenergetic. The experimental period was 42 days. The average daily gain (ADG), average daily feed intake (ADFI) and feed/gain(F/G) of each group were measured at 1 to 21 days of age and 1 to 42 days of age, respectively, the tibia length (TL) was measured at 21 and 42 days of age, and the blood samples were collected to measure serum biochemical and antioxidant indexes at 21 and 42 days of age. The results showed as follows:1) the ADG of G3 group at 1 to 21 days of age and 1 to 42 days of age was significantly higher than that of G0 group (P<0.05), the F/G of G3 group at 1 to 21 days of age was significantly lower than that of G0 group (P<0.05). The ADFI at 1 to 21 days of age and 1 to 42 days of age and TL at 21 and 42 days of age were not significantly different among groups (P>0.05). Serum total protein (TP) and globulin (GLOB) contents of G3 and G6 groups were significantly higher than those of G0 group (P<0.05), and serum urea (UN) content of G3 group was significantly higher than that of G0 group (P<0.05), while serum triglyceride (TG) content of G3 and G6 groups was significantly lower than that of G0 group (P<0.05). 3) Serum total antioxidant capacity (T-AOC) at 21 and 42 days of age of G6 and G9 groups was significantly higher than that of G0 group (P<0.05). Compared with G0 group, the activity of serum glutathione peroxidase (GSH-Px) at 21 and 42 days of age of G3, G6 and G9 groups was significantly increased (P<0.05), and the content of serum malondialdehyde (MDA) was significantly decreased (P<0.05 or P<0.01). There was no significant difference in serum total superoxide dismutase activity at 21 and 42 days of age among groups (P>0.05). In conclusion, under the conditions of this experiment, when equal energy and nitrogen are used to optimize the formula, it is feasible to replace protein feed materials such as soybean meal with the black soldier fly larvae meal to make the diet of layer chickens, and 3% of black soldier fly larvae meal is the best additive.

Key words： black soldier fly larvae meal; layer chickens; growth performance; serum biochemical indexes; antioxidant capacity

Cite this article

WANG Guiying , WANG Kuiming , LI Lusheng , LIU Cheng , GAO Pengxiang , DONG Bingqiang . Effects of Black Soldier Fly Larvae Meal on Growth Performance, Serum Biochemical Indexes and Antioxidant Capacity of Layer Chickens[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3615 -3623 . DOI: 10.3969/j.issn.1006-267x.2020.08.020

References

[1] 中国畜牧业信息网.2018年中国对美农产品进出口贸易额分析[EB/OL].(2018-04-28)[2019-05-07].http://www.chyxx.com/industry/201804/635390.html.
[2] DE MARCO M,MARTÍNEZ S,HERNANDEZ F,et al.Nutritional value of two insect larval meals (Tenebrio molitor and Hermetia illucens) for broiler chickens:apparent nutrient digestibility,apparent ileal amino acid digestibility and apparent metabolizable energy[J].Animal Feed Science and Technology,2015,209:211-218.
[3] MARONO S,LOPONTE R,LOMBARDI P,et al.Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age[J].Poultry Science,2017,96(6):1783-1790.

[4] DABBOU S,GAI F,BIASATO I,et al.Black soldier fly defatted meal as a dietary protein source for broiler chickens:effects on growth performance,blood traits,gut morphology and histological features[J].Journal of Animal Science and Biotechnology,2018,9:49.
[5] SECCI G,BOVERA F,NIZZA S,et al.Quality of eggs from Lohmann Brown Classic laying hens fed black soldier fly meal as substitute for soya bean[J].Animal,2018,12(10):2191-2197.

[6] MWANIKI Z,NEIJAT M,KIARIE E.Egg production and quality responses of adding up to 7.5% defatted black soldier fly larvae meal in a corn-soybean meal diet fed to Shaver White Leghorns from wk 19 to 27 of age[J].Poultry Science,2018,97(8):2829-2835.

[7] MAKKAR H P S,TRAN G,HEUZÉ V,et al.State-of-the-art on use of insects as animal feed[J].Animal Feed Science and Technology,2014,197:1-33.
[8] GASCO L,BIASATO I,DABBOU S,et al.Animals fed insect-based diets:state-of-the-art on digestibility,performance and product quality[J].Animals,2019,9(4):170.
[9] BONDARI K,SHEPPARD D C.Soldier fly larvae as feed in commercial fish production[J].Aquaculture,1981,24:103-109.
[10] SEALEY W M,GAYLORD T G,BARROWS F T,et al.Sensory analysis of rainbow trout,Oncorhynchus mykiss,fed enriched black soldier fly Prepupae,Hermetia illucens[J].Journal of the World Aquaculture Society,2011,42(1):34-45.

[11] 许彦腾,张建新,宋真真,等.黑水虻幼虫蛋白质的制备及体外抗氧化活性[J].核农学报,2014,28(11):2001-2009.
[12] HU J R,WANG G X,HUANG Y H,et al.Effects of substitution of fish meal with black soldier fly (Hermetia illucens) larvae meal,in yellow catfish (Pelteobagrus fulvidraco) diets[J].The Israeli Journal of Aquaculture-Bamidgeh,2017,69:1382-1390.
[13] SHUMO M,OSUGA I M,KHAMIS F M,et al.The nutritive value of black soldier fly larvae reared on common organic waste streams in Kenya[J].Scientific Report,2019,9:10110.
[14] GARIGLIO M,DABBOU S,CRISPO M,et al.Effects of the dietary inclusion of partially defatted black soldier fly (Hermetia illucens) meal on the blood chemistry and tissue (spleen,liver,thymus,and bursa of Fabricius) histology of muscovy ducks (Cairina moschata domestica)[J].Animals,2019,9(6):307
[15] YU M,LI Z M,CHEN W D,et al.Use of Hermetia illucens larvae as a dietary protein source:Effects on growth performance,carcass traits,and meat quality in finishing pigs[J].Meat Science,2019,10:107837.
[16] 张放,朱建平,张政,等.黑水虻虫粉对育肥猪生长性能、血清指标和养分消化率的影响[J].河南农业科学,2017,46(6):130-133,146.
[17] 陈巍,朱剑锋,周海泳,等.黑水虻幼虫虫粉替代仔猪饲料中血浆蛋白粉或鱼粉对仔猪生长性能的影响[J].饲料工业,2017,38(24):36-39.
[18] MUSZY?SKI S,TOMASZEWSKA E,KWIECIE? M,et al.Effect of dietary phytase supplementation on bone and hyaline cartilage development of broilers fed with organically complexed copper in a Cu-deficient diet[J].Biological Trace Element Research,2018,182(2):339-353.

[19] 张放,杨伟丽,杨树义,等.黑水虻虫粉对生长猪生长性能和血清生化指标的影响[J].动物营养学报,2018,30(6):2346-2351.
[20] SCHIAVONE A,DE MARCO M,MARTÍNEZ S,et al.Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens:apparent nutrient digestibility,apparent metabolizable energy and apparent ileal amino acid digestibility[J].Journal of Animal Science and Biotechnology,2017,8:51.
[21] 胡俊茹,王国霞,莫文艳,等.黑水虻幼虫粉替代鱼粉对鲈鱼幼鱼生长性能、体组成、血浆生化指标和组织结构的影响[J].动物营养学报,2018,30(2):613-623.
[22] 陈晓瑛,胡俊茹,王国霞,等.黑水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉品质及血清生化指标的影响[J].动物营养学报,2019,31(6):2788-2799.
[23] KROECKEL S,HARJES A G E,ROTH I,et al.When a turbot catches a fly:evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute-Growth performance and chitin degradation in juvenile turbot (Psetta maxima)[J].Aquaculture,2012,364/365:345-352.
[24] LI X,RAHIMNEJAD S,WANG L,et al.Substituting fish meal with housefly (Musca domestica) maggot meal in diets for bullfrog Rana (Lithobates) catesbeiana:effects on growth,digestive enzymes activity,antioxidant capacity and gut health[J].Aquaculture,2019,499:295-305.
[25] WYNANTS E,FROONINCKX L,VAN MIERT S,et al.Risks related to the presence of Salmonella sp.during rearing of mealworms (Tenebrio molitor) for food or feed:survival in the substrate and transmission to the larvae[J].Food Control,2019,100:227-234.
[26] 吉红,李森林,徐歆歆.昆虫资源在水产饲料中的应用研究进展[J].饲料工业,2016,37(22):1-9.
[27] ALEGBELEYE W O,OBASA S O,OLUDE O O,et al.Preliminary evaluation of the nutritive value of the variegated grasshopper (Zonocerus variegatus L.) for African catfish Clarias gariepinus (Burchell.1822) fingerlings[J].Aquaculture Research,2012,43(3):412-420.

[28] POZZO L,SALAMANO G,MELLIA E,et al.Feeding a diet contaminated with ochratoxin A for chickens at the maximum level recommended by the EU for poultry feeds (0.1 mg/kg).1.Effects on growth and slaughter performance,haematological and serum traits[J].Journal of Animal Physiology and Animal Nutrition,2013,97(Suppl.1):13-22.
[29] TANG Z R,YIN Y L,NYACHOT C M,et al.Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-Ⅰ mRNA expression in early-weaned piglets[J].Domestic Animal Endocrinology,2005,28(4):430-441.

[30] METGES C C,PETZKE K J,HENNIG U.Gas chromatography/combustion/isotope ratio mass spectrometric comparison of N-acetyl-and N-pivaloyl amino acid esters to measure ¹⁵N isotopic abundances in physiological samples:a pilot study on amino acid synthesis in the upper gastro-intestinal tract of minipigs[J].Journal of Mass Spectrometry,1996,31(4):367-376.

[31] MU C L,YANG Y X,LUO Z,et al.Metabolomic analysis reveals distinct profiles in the plasma and urine of rats fed a high-protein diet[J].Amino Acids,2015,47(6):1225-1238.

[32] 余苗,李贞明,陈卫东,等.黑水虻幼虫粉对育肥猪营养物质消化率、血清生化指标和氨基酸组成的影响[J].动物营养学报,2019,31(7):3330-3337
[33] VALLE E,ZANATTA R,ODETTI P,et al.Effects of competition on acute phase proteins and lymphocyte subpopulations-oxidative stress markers in eventing horses[J].Journal of Animal Physiology and Animal Nutrition,2015,99(5):856-863.

[34] RADI R.Protein tyrosine nitration:biochemical mechanisms and structural basis of functional effects[J].Accounts of Chemical Research,2013,46(2):550-559.

[35] LI S L,JI H,ZHANG B X,et al.Defatted black soldier fly (Hermetia illucens) larvae meal in diets for juvenile Jian carp (Cyprinus carpio var. Jian):growth performance,antioxidant enzyme activities,digestive enzyme activities,intestine and hepatopancreas histological structure[J].Aquaculture,2017,477:62-70.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References