Probiotic Characteristics of Lactobacillus reuteri ZJF036 Isolated from Fox

  • ZHANG Ruchun ,
  • CUI Huanzhong ,
  • CAI Xiheng ,
  • WANG Jinming ,
  • LIU Chaonan ,
  • WANG Jing ,
  • WANG Xiaoxu ,
  • LIU Hanlu
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  • 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
    2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Sciences, Changchun 130112, China

Received date: 2020-02-13

  Online published: 2020-08-13

Abstract

The objective of this study was to investigate the fermentation characteristics, adhesion ability and animal safety of Lactobacillus reuteri ZJF036 isolated from fox, and to explore the prebiotic property of Lactobacillus reuteri ZJF036. The growth characteristics, acid-producing capacity, drug sensitivity, tolerance to artificial gastric juice, artificial intestinal juice and bile salts, hydrophobicity and self-coacervation rates, adhesion ability to fox intestinal epithelial cells, and mouse blood physiological and biochemical indexes of Lactobacillus reuteri ZJF036 were detected. The results showed that the logarithmic growth phase is 4 to 10 h for bacteria culture of Lactobacillus reuteri ZJF036, the minimum pH of culture medium is 3.80; Lactobacillus reuteri ZJF036 was sensitive to midecamycin, ceftriaxone and chloramphenicol, but not sensitive to vancomycin, ciprofloxacin, amikacin and ampicillin; the supernatant of bacterial culture had good bacteriostatic effects on Escherichia coli, Salmonella and Staphylococcus aureus; the survival rate of Lactobacillus reuteri ZJF036 after culturing with simulated artificial gastric juice and artificial intestinal juice for 3 h was over 90%, and it could tolerate 0.4% bile salt; the hydrophobicity of Lactobacillus reuteri ZJF036 was 82.27%, and the self-coacervation rates at 2 and 4 h were 32.47% and 41.70%, respectively; the adhesion number of Lactobacillus reuteri ZJF036 to fox intestinal epithelial cells was (77.0±8.0) CFU/cell, which was significantly higher than that of Lactobacillus rhamnosus GG isolated from chicken; in addition, the safety test on mouse showed that blood physiological and biochemical indexes were normal. It is concluded that Lactobacillus reuteri ZJF036 grows rapidly, has good acid-producing capacity and bacteriostatic ability, can tolerate artificial gastric juice, artificial intestinal juice and bile salt, has strong adhesion to fox small intestinal epithelial cells, and is safe and reliable to mice. Lactobacillus reuteri ZJF036 is expected to be a candidate strain for preparation of canine probiotics.

Cite this article

ZHANG Ruchun , CUI Huanzhong , CAI Xiheng , WANG Jinming , LIU Chaonan , WANG Jing , WANG Xiaoxu , LIU Hanlu . Probiotic Characteristics of Lactobacillus reuteri ZJF036 Isolated from Fox[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3819 -3829 . DOI: 10.3969/j.issn.1006-267x.2020.08.041

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