Effects of Long-Term Selenium Deficiency on D-Serine and L-Serine Levels in Rats

YU Haili; ZHANG Fengwei; ZHANG Yan; ZHANG Shuangqing

doi:10.3969/j.issn.1006-267x.2020.08.045

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 8: 3869 - 3876

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.045

Effects of Long-Term Selenium Deficiency on D-Serine and L-Serine Levels in Rats

YU Haili ,
ZHANG Fengwei ,
ZHANG Yan ,
ZHANG Shuangqing

Expand

1. The Hubei Provincial Key Laboratory of Yeast Function, Yichang 443003, China;
2. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Received date: 2020-02-20

Online published: 2020-08-13

Fold

Abstract

This experiment was conducted to establish a selenium deficiency rat model and investigate effects of long-term selenium deficiency on D-serine and L-serine levels in rats. Twelve male weaned rats aged 3 weeks were randomly divided into 2 groups and each group had 6 rats. Rats in the selenium deficiency group were fed a low selenium diet (selenium level of 0.02 mg/kg) and rats in the control group were fed a normal diet (selenium level of 0.18 mg/kg). The experiment lasted for 300 days. Selenium level in plasma were determined using inductively coupled plasma mass spectrometry. High performance liquid chromatography was used to analyze the levels of D-serine and L-serine after derivatizating with o-phthalaldehyde and N-acetyl-L-cysteine. The results showed as follows:1) under the mobile phase consisting of 50 mmol/L of ammonium acetate and methanol, D-serine and L-serine were successfully separated on the Agilent ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm). 2) Compared with the rats fed normal diet, plasma selenium level of rats fed selenium deficiency diet was significantly decreased (P<0.01), and plasma D-serine, L-serine levels as well as cerebellum L-serine level were increased, but differences were not significant (P>0.05). In conclusion, the selenium deficiency rat model is successfully established by selenium-deficient diets, and D-serine, L-serine levels in plasma and cerebellum are not significantly affected by the long-term selenium deficiency.

Key words： selenium; D-serine; L-serine

Cite this article

YU Haili , ZHANG Fengwei , ZHANG Yan , ZHANG Shuangqing . Effects of Long-Term Selenium Deficiency on D-Serine and L-Serine Levels in Rats[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3869 -3876 . DOI: 10.3969/j.issn.1006-267x.2020.08.045

References

[1] ROMAN M,JITARU P,BARBANTE C.Selenium biochemistry and its role for human health[J].Metallomic,2014,6(1):25-54.

[2] YANG Z J,LIU C,LIU C P,et al.Selenium deficiency mainly influences antioxidant selenoproteins expression in broiler immune organs[J].Biological Trace Element Research,2015,172(1):209-221.
[3] 谢斌,吴文良,郭岩彬,等.作物富硒研究进展[J].江苏农业科学,2014,42(1):15-17,18.
[4] DUNTAS L H,BENVENGA S.Selenium:an element for life[J].Endocrine,2015,48(3):756-775.

[5] 王磊,杜菲,孙卉,等.人体硒代谢与硒营养研究进展[J].生物技术进展,2015,5(4):285-290.
[6] KALHAN S C,HANSON R W.Resurgence of serine:an often neglected but indispensable amino acid[J].Journal of Biological Chemistry,2012,287(24):19786-19791.

[7] WANG Q,SUN L C,LIU Y Q,et al.The synergistic effect of serine with selenocompounds on the expression of SelP and GPx in HepG2 cells[J].Biological Trace Element Research,2016,173(2):291-296.

[8] 谭茹尹,韦庆臣,罗佐杰.D-丝氨酸的氧化代谢途径与疾病关系的研究进展[J].广东医学,2018,39(10):1575-1577,1582.
[9] AKAHOSHI N,ANAN Y,HASHIMOTO Y,et al.Dietary selenium deficiency or selenomethionine excess drastically alters organ selenium contents without altering the expression of most selenoproteins in mice[J].The Journal of Nutritional Biochemistry,2019,69:120-129.
[10] MICKIEWICZ B,VILLEMAIRE M L,SANDERCOCK L E,et al.Metabolic changes associated with selenium deficiency in mice[J].Biometals,2014,27(6):1137-1147.

[11] 田俊梅,张丁,付瑞娟,等.大豆硒蛋白与亚硒酸钠生物利用的比较研究[J].大豆科学,2010,29(3):534-536.
[12] 傅瑞娟,张锐,田俊梅,等.大鼠对亚硒酸钠和富硒酵母吸收利用的比较研究[J].现代预防医学,2011,38(1):26-29.
[13] 余海立,陈智仙,张彦,等.硒与认知功能[J].中国药事,2019,33(4):385-390.
[14] 王艳,苏敏.硒缺乏动物模型的建立及应用[J].环境与职业医学,2004,21(4):287-290.
[15] FREZZA C.Cancer metabolism:addicted to serine[J].Nature Chemical Biology,2016,12(6):389-390.

[16] HOLM L J,BUSCHARD K.L-serine:a neglected amino acid with a potential therapeutic role in diabetes[J].Journal of Pathology,Microbiology and Immunology,2019,127(10):655-659.
[17] EL-HATTAB A W.Serine biosynthesis and transport defects[J].Molecular Genetics and Metabolism,2016,118(3):153-159.

[18] STEINBRENNER H,SIES H.Protection against reactive oxygen species by selenoproteins[J].Biochimica et Biophysica Acta:General Subjects,2009,1790(11):1478-1485.

[19] MARALANI M N,MOVAHEDIAN A,JAVANMARD S H.Antioxidant and cytoprotective effects of L-serine on human endothelial cells[J].Research in Pharmaceutical Sciences,2012,7(4):209-215.
[20] GUEVARA C M,MANI A R.The role of D-serine in peripheral tissues[J].European Journal of Pharmacology,2016,780:216-223.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References