Repairing Effects of Goose Oil Diglyceride Combined with Cecropin Antimicrobial Peptide on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

XU Huixin; WANG Baowei; GE Wenhua; CONG Hongxia; WANG Qian; ZHANG Ming; KONG Min; FAN Wenlei

doi:10.3969/j.issn.1006-267x.2020.08.046

2020 , Vol. 32 >Issue 8: 3877 - 3886

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.046

Repairing Effects of Goose Oil Diglyceride Combined with Cecropin Antimicrobial Peptide on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

XU Huixin ,
WANG Baowei ,
GE Wenhua ,
CONG Hongxia ,
WANG Qian ,
ZHANG Ming ,
KONG Min ,
FAN Wenlei

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1. Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2020-03-20

Online published: 2020-08-13

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Abstract

This experiment was conducted to study the effects of goose oil diglyceride (GDG) combined with cecropin antimicrobial peptide (CAP) on dextran sodium sulfate (DSS)-induced ulcerative colitis injury in mice. The experiment was divided into two phases. Modeling phase:a total of 184 mice were randomly divided into 8 groups, which were control group (group Ⅰ), model group (group Ⅱ) and repair groups (groups Ⅲ to Ⅷ). Each control group and model group contained 4 replicates and 8 mice per replicate; each repair group contained 4 replicates and 5 mice per replicate. Mice in the control group were free to drink distilled water, and others in model group and repair groups were free to drink 5% DSS aqueous solution to induce the intestinal inflammation of mice, it lasted for 1 week. Repair phase:a total of 160 mice were randomly divided into control group (group Ⅰ), model group (group Ⅱ) and repair groups (groups Ⅲ to Ⅷ), each group contained 4 replicates and 5 mice per replicate. Mice in the control group and model group were gavage with normal saline (100 μL per mouse); mice repair groups were gavage with different levels of GDG and CAP, the GDG supplemental levels were 50, 75, and 100 μL per mouse, respectively; and the CAP supplemental levels were 3.75 and 5.00 mL/kg BW, respectively. The results showed as follows:1) compared with group Ⅰ, the body weight after modeling of group Ⅱ was significantly decreased (P<0.05), the disease activity index (DAI) score after modeling was significantly increased (P<0.05), the colonic length was significantly decreased (P<0.05), and the contents of interleukin-6 and tumor necrosis factor-α (TNF-α) in serum were significantly increased (P<0.05), it showed that the ulcerative colitis mice model successful build. 2) The body weight after repairing of groups Ⅲ to Ⅷ was significantly higher than that of group Ⅱ (P<0.05), and the DAI score after repairing was significantly lower than that of group Ⅱ (P<0.05). 3) The colonic length of groups Ⅲ to Ⅷ was significantly higher than that of group Ⅱ (P<0.05), the colonic villus height was significantly higher than that of group Ⅱ (P<0.05), the colonic crypt depth was significantly lower than that of group Ⅱ (P<0.05), and the colonic villus height/crypt depth was significantly higher than that of group Ⅱ (P<0.05). 4) GDG combined with CAP had significant interaction on contents of interleukin-4, interleukin-6, interleukin-17, interleukin-1β and TNF-α in serum (P<0.05). In conclusion, GDG combined with CAP can effectively treat ulcerative colitis of mice, the mice gavage with 75 μL per mouse GDG+5.00 mL/kg BW CAP have the best repair effect on ulcerative colitis injury.

Key words： goose oil diglyceride; cecropin antibacterial peptide; mice; ulcerative colitis; repair

Cite this article

XU Huixin , WANG Baowei , GE Wenhua , CONG Hongxia , WANG Qian , ZHANG Ming , KONG Min , FAN Wenlei . Repairing Effects of Goose Oil Diglyceride Combined with Cecropin Antimicrobial Peptide on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3877 -3886 . DOI: 10.3969/j.issn.1006-267x.2020.08.046

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