Effects of Weaning Time on Microbial Community Structure and Their Functions in Rumen of Lambs

  • LI Haiqin ,
  • JIA Jianlei ,
  • CHEN Qian ,
  • HOU Shengzhen ,
  • GUI Linsheng
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  • College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

Received date: 2020-02-23

  Online published: 2020-08-13

Abstract

The purpose of this experiment was to study the effects of weaning time on the rumen microbial community structure and their functions of lambs. In this study, 90 mall tail cold lambs in Haidong area of Qinghai province with the similar initial condition were randomly divided into 3 groups, each group had 30 lambs, weaned in 30, 40 and 50 days of age, respectively, and reared to 90 days of age after the random slaughter of 6 animals, and then obtained rumen content samples to analyze the differences in the structure of the stomach fluid volatile fatty acid (VFA) contents and rumen microbial community structure and their functions. The results showed as follows:1) weight at 90 days of age and average daily weight gain in weaning at 40 days of age group were significantly higher than those in the other two groups (P<0.05). 2) Rumen fluid VFA and acetic acid content, acetic acid/butyric acid in weaning at 40 days of age group were significantly higher than those in the other two groups (P<0.05), the content of propionic acid was significantly lower than that in the other two groups (P<0.05), and isobutyric acid and butyric acid contents gradually decreased with the extension of weaning time(P<0.05). 3) Christensenellaceae R-7 group, Ruminococcaceae_NK4A214_group and Prevotella 1 were dominant bacteria for this experiment; lamb rumen bacteria diversity in weaning at 40 days of age group was higher than that in the other two groups; the relative abundance of Butyrivirio 2, Ruminococcaceae_NK4A214_group, Ruminococcus 2, Lachnospiraceae_NK3A20_group and Lachnospiraceae_XPB1014_group in weaning at 40 days of age group was significantly higher than that in the other two groups (P<0.05), and the relative abundance of Prevotella 1 and Rikenellaceae RC9 gut group in weaning at 40 days of age group were significantly lower than those in the other two groups (P<0.05). 4) Clusters of orthologous groups of proteins (COG) function found that lamb rumen bacteria function was mainly concentrated in carbohydrate and amino acid transport and metabolism. In summary, rumen microorganism (bacterial) system of lambs in weaing at 40 days of age group is more perfect, which is conducive to the growth and development of lambs, and can be used as the ideal weaning time of small tail cold sheep in Haidong area in the actual production.

Cite this article

LI Haiqin , JIA Jianlei , CHEN Qian , HOU Shengzhen , GUI Linsheng . Effects of Weaning Time on Microbial Community Structure and Their Functions in Rumen of Lambs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(8) : 3952 -3960 . DOI: 10.3969/j.issn.1006-267x.2020.08.054

References

[1] 任春燕.不同NDF水平的开食料对犊牛生长、屠宰性能、抗氧化、免疫及胃肠道发育的影响[D].博士学位论文.兰州:甘肃农业大学,2018.
[2] 解彪.不同NDF水平饲粮对羔羊生长性能和瘤胃发育的影响[D].硕士学位论文.晋中:山西农业大学,2018.
[3] 符运勤.地衣芽孢杆菌及其复合菌对后备牛生长性能和瘤胃内环境的影响[D].硕士学位论文.北京:中国农业科学院,2012.
[4] 郭峰.断母乳日龄及营养水平对肉犊牛生长性能与瘤胃发酵的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2015
[5] 陈昊,刘婷,吴建平,等.牛至精油对新生犊牛生长发育和血液免疫的影响[J].草业科学,2017,34(10):2141-2148.
[6] 罗惦,柴林荣,常生华,等.我国青藏高原地区牦牛草地放牧系统管理及优化[J].草业科学,2017,34(4):881-891.
[7] 肖怡.三种益生菌对肉羊甲烷排放、物质代谢和瘤胃发酵的影响[D].硕士学位论文.阿拉尔:塔里木大学,2016.
[8] 郭江鹏,张元兴,李发弟,等.0-56日龄舍饲肉用羔羊胃肠道发育特点研究[J].畜牧兽医学报,2011,42(4):513-520.
[9] 祁敏丽,刁其玉,张乃锋.羔羊瘤胃发育及其影响因素研究进展[J].中国畜牧杂志,2015,51(9):77-81.
[10] 王剑.干草添加时期对哺乳期犊牛生长性能和瘤胃发育的影响[D].硕士学位论文.泰安:山东农业大学,2015.
[11] 马万浩,张宁,李飞,等.断奶日龄对湖羊羔羊瘤胃内微生物多样性的影响[J].动物营养学报,2018,30(8):3055-3062.
[12] LIANG Y S,LI G Z,LI X Y,et al.Growth performance,rumen fermentation,bacteria composition,and gene expressions involved in intracellular pH regulation of rumen epithelium in finishing Hu lambs differing in residual feed intake phenotype[J].Journal of Animal Science,2017,95(4):1727-1738.
[13] 朱文涛.15-150日龄羔羊日粮消化率和瘤胃消化功能变化的研究[D].博士学位论文.乌鲁木齐:新疆农业大学,2005.
[14] 王志有,侯生珍,赵小龙.早期断奶日龄对藏羔羊生长发育的影响[J].黑龙江畜牧兽医,2015(22):66-68.
[15] RUSSELL J B,RYCHLIK J L.Factors that alter rumen microbial ecology[J].Science,2001,292(5519):1119-1122.  
[16] 靳文尧.瘤胃微生物厌氧消化农业固体有机废物技术与应用研究[D].博士学位论文.大连:大连理工大学,2018.
[17] 曹少奇.早期断奶对哈萨克羔羊生长性能及胃肠道发育的影响[D].硕士学位论文.石河子:石河子大学,2016.
[18] LI M J,ZHOU M,ADAMOWICZ E,et al.Characterization of bovine ruminal epithelial bacterial communities using 16S rRNA sequencing,PCR-DGGE,and qRT-PCR analysis[J].Veterinary Microbiology,2012,155(1):72-80.  
[19] 柴建民.断母乳日龄对羔羊生长性能与胃肠道发育的影响[D].硕士学位论文.北京:中国农业科学院,2015.
[20] KAMRA D N.Rumen microbial ecosystem[J].Current Science,2005,89(1):124-135.
[21] WOOD J,SCOTT K P,AVGUSTIN G,et al.Estimation of the relative abundance of different Bacteroides and Prevotella ribotypes in gut samples by restriction enzyme profiling of PCR-amplified 16S rRNA gene sequences[J].Applied and Environmental Microbiology,1998,64(10):3683-3689.  
[22] MATSUI H,OGATA K,TAJIMA K,et al.Phenotypic characterization of polysaccharidases produced by four Prevotella type strains[J].Current Microbiology,2000,41(1):45-49.  
[23] HOOPER L V,WONG M H,THELIN A,et al.Molecular analysis of commensal host-microbial relationships in the intestine[J].Science,2001,291(5505):881-884
[24] BÄCKHED F,LEY R,SONNENBURG J,et al.The human intestinal microbiota and its relationship to energy balance[J].Food & Nutrition,2006,50(Suppl.2):121-123.
[25] BACKHED F,DING H,WANG T,et al.The gut microbiota as an environmental factor that regulates fat storage[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(44):15718-15723.  
[26] 高凤.奶牛肠道微生物群落结构与多样性研究[D].硕士学位论文.邯郸:河北工程大学,2017.
[27] 张莉.日粮纤维对仔猪肠道屏障功能的影响及调控机制的研究[D].博士学位论文.北京:中国农业科学院,2018.
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