Effects of Schizochytrium sp. and Lactoferrin on Diarrhea, Growth Performance, Fecal Score and Serum Antioxidant Indices of Sucking Calves Challenged with Escherichia coli K99

Alatengzhula; LIU Wenhui; MA Lu; REN Lijuan; GAO Shengtao; BU Dengpan

doi:10.3969/j.issn.1006-267x.2020.09.026

2020 , Vol. 32 >Issue 9: 4166 - 4176

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.026

Effects of Schizochytrium sp. and Lactoferrin on Diarrhea, Growth Performance, Fecal Score and Serum Antioxidant Indices of Sucking Calves Challenged with Escherichia coli K99

Alatengzhula ,
LIU Wenhui ,
MA Lu ,
REN Lijuan ,
GAO Shengtao ,
BU Dengpan

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Chinese Academy of Agricultural Sciences and World Agroforestry Center Joint Lab on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, China

Received date: 2020-03-21

Online published: 2020-09-17

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Abstract

This study aimed to determine the alleviating effects of Schizochytrium sp. and lactoferrin on diarrhea of sucking calves challenged with Escherichia coli K99, and the effects of Schizochytrium sp. and lactoferrin on growth performance and serum antioxidant indices of sucking calves. Fifty 1-day-old newborn male Holstein calves were randomly assigned to 5 groups with 10 calves per group. The control group (C group) was fed a basic diet without Schizochytrium sp. and lactoferrin, the lactoferrin group (LF group) was fed the basic diet+1 g/d lactoferrin, the Schizochytrium sp. group (SZ group) was fed the basic diet+20 g/d Schizochytrium sp., and the lactoferrin+Schizochytrium sp. group (LF+SZ group) was fed the basic diet+1 g/d lactoferrin+20 g/d Schizochytrium sp. At the same time, the non-challenged E. coli group was set as reference. On 7 days of age, all the calves were challenged with Escherichia coli K99 (10 mL, 1×10¹¹ CFU/mL) orally except the non-challenged Escherichia coli group, and the experiment ended at 14 days of age. The results showed as follows:1) during 1 to 7 days of age, dietary Schizochytrium sp tended to increase the average daily gain (P=0.093); during 8 to 11 days of age, compared with C group, the fecal score of LF, SZ and LF+SZ groups was significantly decreased (P<0.05), and lactoferrin and Schizochytrium sp had a synergistic action tendency on decreasing fecal score (P=0.074). 2) At 11 days of age, compared with C group, the serum catalase (CAT) activity of LF, SZ and LF+SZ groups was significantly increased (P<0.05), and LF+SZ group was significantly higher than LF and SZ groups (P<0.05); at 14 days of age, the serum CAT activity of LF+SZ group was significantly higher than that of SZ and C groups (P<0.05). At 14 days of age, compared with C group, the serum superoxide dismutase (SOD) activity of SZ and LF+SZ groups was significantly increased (P<0.05). At 7 days of age, compared with SZ and LF+SZ group, the serum glutathione peroxidase (GSH-Px) activity of LF group was significantly increased (P<0.05). At 11 days of age, compared with LF group, the serum malondialdehyde (MDA) content of SZ and LF+SZ groups was significantly increased (P<0.05); at 14 days of age, the serum MDA content of LF+SZ group was significantly higher than that of other groups (P<0.05). In conclusion, when sucking calves are under the condition of challenged with Escherichia coli K99, dietary Schizochytrium sp. and lactoferrin can promote intestinal health, reduce diarrhea and improve antioxidant capacity. Lactoferrin is better than Schizochytrium sp. on improving the antioxidant capacity of calves, and Schizochytrium sp. combined with lactoferrin have synergistic action on reduce the fecal score of sucking calves, and effectively alleviated diarrhea of calves.

Key words： Schizochytrium sp.; lactoferrin; sucking calves; fecal score; antioxidant capacity

Cite this article

Alatengzhula , LIU Wenhui , MA Lu , REN Lijuan , GAO Shengtao , BU Dengpan . Effects of Schizochytrium sp. and Lactoferrin on Diarrhea, Growth Performance, Fecal Score and Serum Antioxidant Indices of Sucking Calves Challenged with Escherichia coli K99[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4166 -4176 . DOI: 10.3969/j.issn.1006-267x.2020.09.026

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