Effects of Methionine and Biotin on Growth of Secondary Follicles in Cashmere Goat

LIN Ying; WANG Wennan; LIU Haiying; ZHU Yanxu; ZHANG Xiaoying; WANG Zhanhong

doi:10.3969/j.issn.1006-267x.2020.09.032

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 9: 4222 - 4229

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.032

Effects of Methionine and Biotin on Growth of Secondary Follicles in Cashmere Goat

LIN Ying ,
WANG Wennan ,
LIU Haiying ,
ZHU Yanxu ,
ZHANG Xiaoying ,
WANG Zhanhong

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1. College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China;
2. Liaoning Province Modern Agricultural Production Base and Construction Engineering Center, Liaoyang 111000, China

Received date: 2020-03-27

Online published: 2020-09-17

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Abstract

The purpose of this study was to investigate the effects and functions of methionine and biotin on the growth and morphology of secondary hair follicles in cashmere goats. The secondary follicles of cashmere goats were separated by enzymatic method and in vitro mechanical separation technology, and secondary hair follicles with complete shape were selected for in vitro culture of hair follicles. Experiment 1:isolated intact hair follicles were randomly divided into 5 groups using a single factor test design, each with 4 repetitions, each with 12 hair follicles, a total of 240 secondary hair follicles, the control group was Williams E medium, and the test group was added with 7.5, 15.0, 30.0 and 60.0 mg/L methionine. Experiment 2:isolated intact hair follicles were randomly divided into 4 groups using a single factor test design, each with 3 repetitions, each with 10 hair follicles, a total of 120 secondary hair follicles, the control group was Williams E medium, and the test group was added with 0.25, 0.50, 1.00 mg/L biotin. All were cultured for 7 days, and the hair follicle growth length was measured every 24 h during the culture period, and photos were taken to determine the amount of hair follicle and DNA synthesis after the test. The results of experiment 1 showed as follows:compared with the control group, the 15 mg/L methionine significantly increased the secondary hair follicle growth length and growth rate (P<0.05), and the shape of the hair bulb remained the most. The DNA content in the 0.5 mg/L biotin group was the highest. The results of experiment 2 showed as follows:compared with the control group, the 0.50 mg/L biotin significantly increased the secondary hair follicle growth length and growth rate (P<0.05), and the secondary hair follicle DNA content in the 0.50 mg/L biotin group was the highest. From this, it can be seen that adding 15.0 mg/L methionine and 0.50 mg/L biotin can significantly increase the growth length and growth rate of hair follicles; and the secondary hair follicle DNA content of these two groups is the highest.

Key words： methionine; biotin; secondary follicles; in vitro culture; cashmere goat

Cite this article

LIN Ying , WANG Wennan , LIU Haiying , ZHU Yanxu , ZHANG Xiaoying , WANG Zhanhong . Effects of Methionine and Biotin on Growth of Secondary Follicles in Cashmere Goat[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4222 -4229 . DOI: 10.3969/j.issn.1006-267x.2020.09.032

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