Effects of Dietary &gamma;-Aminobutyric Acid Supplemental Level on Growth Performance, Serum Biochemical Indices and Anti-Nitrite-Nitrogen Stress Ability of Litopenaeus vannamei

ZHAO Hongxia; CHEN Xiaoying; WANG Guoxia; PENG Kai; HUANG Yanhua; CHEN Bing; CAO Junming

doi:10.3969/j.issn.1006-267x.2020.09.035

2020 , Vol. 32 >Issue 9: 4251 - 4259

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.035

Effects of Dietary γ-Aminobutyric Acid Supplemental Level on Growth Performance, Serum Biochemical Indices and Anti-Nitrite-Nitrogen Stress Ability of Litopenaeus vannamei

ZHAO Hongxia ,
CHEN Xiaoying ,
WANG Guoxia ,
PENG Kai ,
HUANG Yanhua ,
CHEN Bing ,
CAO Junming

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1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China;
3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2020-03-25

Online published: 2020-09-17

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Abstract

This experiment was conducted to investigate the effects of dietary γ-aminobutyric acid (GABA) supplemental level on growth performance, serum biochemical indices and anti-nitrite-nitrogen stress ability of Litopenaeus vannamei. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of GABA (0, 50, 100, 150, 200 and 250 mg/kg of diet), by adding GABA. Nine hundred and sixty Litopenaeus vannamei shrimp with an initial average body weight of (0.68±0.02) g were randomly divided into 6 groups with 4 replicates of 40 shrimp each and each group was fed with one of the diets. After 56 d feeding, shrimp were exposed to nitrite-nitrogen for 120 h. The results showed as follows:1) the weight gain rate (WGR) and feed intake (FI) of shrimp fed diet with 50 to 250 mg/kg GABA were significantly higher than those of shrimp fed diet without GABA (P<0.05). 2) Compared with the no-adding group, diet with 100 and 150 mg/kg GABA significantly increased the contents of serum total protein (TP), triglyceride (TG) and cholesterol (CHO) (P<0.05), and significantly decreased the activities of serum glutamic oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) (P<0.05). 3) The serum lysozyme (LYZ), superoxide dismutase (SOD), catalase (CAT) activities and total antioxidant capacity (T-AOC) in 100 and 150 mg/kg GABA groups were significantly higher than those in no-adding group (P<0.05). 4) After 120 h challenge to nitrite-nitrogen stress, the cumulative mortality rate in 100, 150, 200 and 250 mg/kg GABA groups was significantly lower than that in no-adding group (P<0.05). In conclusion, dietary supplemented with GABA can improve the FI, WGR and anti-nitrite-nitrogen stress ability of Litopenaeus vannamei. Using WGR as the evaluation index, the optimal dietary GABA supplemental level is estimated to be 154.79 mg/kg of diet by quadratic regression analysis.

Key words： γ-aminobutyric acid; Litopenaeus vannamei; growth performance; serum biochemical indices; nitrite-nitrogen stress

Cite this article

ZHAO Hongxia , CHEN Xiaoying , WANG Guoxia , PENG Kai , HUANG Yanhua , CHEN Bing , CAO Junming . Effects of Dietary γ-Aminobutyric Acid Supplemental Level on Growth Performance, Serum Biochemical Indices and Anti-Nitrite-Nitrogen Stress Ability of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4251 -4259 . DOI: 10.3969/j.issn.1006-267x.2020.09.035

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