Effects of Dietary Protein Level on Nutrient Digestibility, Nitrogen Metabolism and Serum Biochemical and Antioxidant Indices of Adult Beagle Dogs

  • LIU Keyuan ,
  • SUN Weili ,
  • LIU Hanlu ,
  • ZHANG Ting ,
  • SHI Hongpeng ,
  • LI Guangyu
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  • Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2020-03-30

  Online published: 2020-09-17

Abstract

This research was conducted to investigate the effects of dietary protein level on nutrient digestibility, nitrogen metabolism and serum biochemical and antioxidant indices of adult beagle dogs. Twenty-four healthy adult male beagle dogs during 14 to 16 months of age with similar body weight were randomly assigned to 3 groups with 8 replicates per group. Dogs in 3 groups were fed amino acid balanced diets with protein levels of 21.08% (group Ⅰ), 22.39% (group Ⅱ) and 25.51% (group Ⅲ), respectively. The experiment lasted for 30 days after 7 days adaption. A digestion metabolism experiment was conducted for 3 d starting at the 28th day of the experiment, and then nearly 5 mL serum samples were collected for the determination of serum biochemical and antioxidant indices. The results showed as follows:1) dietary protein level had no significant effects on digestibilities of dry matter, protein and carbohydrate of adult beagle dogs (P>0.05). The fat digestibility of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). 2) Dietary protein level had no significant effects on fecal nitrogen, urinary nitrogen, retention nitrogen, net protein utilization and biological value of protein of adult beagle dogs (P>0.05). The intake nitrogen of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05). 3) Dietary protein level had no significant effects on total protein, glucose, high density lipoprotein cholesterol and low density lipoprotein cholesterol contents and alanine aminotransferase activity in serum of adult beagle dogs (P>0.05). The serum albumin content of group Ⅱ was significantly higher than that of group Ⅲ (P<0.05), the contents of globulin and urea in serum of group Ⅲ were significantly higher than those of groups Ⅰ and Ⅱ (P<0.05), and the triglycerides and cholesterol contents and aspartate aminotransferase activity in serum of group Ⅲ were significantly lower than those of groups Ⅰ and Ⅱ (P<0.05). 4) The total antioxidant capacity, glutathione peroxidase activity and malondialdehyde content in serum of group Ⅲ were significantly higher than those of groups Ⅰ and Ⅱ (P<0.05), and the serum superoxide dismutase activity was significantly lower than that of groups Ⅰ and Ⅱ (P<0.05). In conclusion, dietary protein level have no significant effects on protein digestibility and nitrogen deposition of adult beagle dogs under the condition of dietary amino acid balance, however, high dietary protein level will lead to the increase of contents of urea and malondialdehyde in serum. When the dietary protein level is 21.08%, it can meet the physiological needs of adult beagle dogs.

Cite this article

LIU Keyuan , SUN Weili , LIU Hanlu , ZHANG Ting , SHI Hongpeng , LI Guangyu . Effects of Dietary Protein Level on Nutrient Digestibility, Nitrogen Metabolism and Serum Biochemical and Antioxidant Indices of Adult Beagle Dogs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4309 -4317 . DOI: 10.3969/j.issn.1006-267x.2020.09.041

References

[1] 刘晓琳.维持犬肠道健康的重要性及措施[J].中国工作犬业,2013(5):12-13.
[2] 李海云,李奎,丁成,等.宠物营养研究现状及发展前景[J].饲料研究,2017,20(2):6-8,12.
[3] GERMAN A J.The growing problem of obesity in dogs and cats[J].The Journal of Nutrition,2006,136(7 Suppl):1940S-1946S.
[4] 陈志敏,王金全,常文环.宠物犬营养需要研究进展[J].饲料工业,2014,35(17):71-75.
[5] 王米,赵支新,孟新宇,等.上海地区宠物食品(犬粮)调查报告[J].环球宠物科技,2008(1):40-45.
[6] AAFCO.AAFCO dog food nutrient profiles[S].Atlanta,GA:Association of American Feed Control Officials,2016.
[7] 李伟忠,李焕江.饲粮中适宜能量蛋白比选择的研究进展[J].饲料博览,2003(4):20-22.
[8] 郑建婷,淡瑞芳,郑中朝.宠物犬蛋白质营养研究进展[J].饲料工业,2008,29(17):48-50.
[9] 周晓容,杨飞云,肖融,等.不同蛋白质水平日粮对荣×大×杜生长猪生产性能和养分消化率的影响[J].中国饲料,2010(16):11-14.
[10] XIAO R J,XU Z R,CHEN H L.Effects of ractopamine at different dietary protein levels on growth performance and carcass characteristics in finishing pigs[J].Animal Feed Science and Technology,1999,79(1/2):119-127.
[11] 梁福广,何欣,马秋刚,等.不同蛋白质水平条件下氨基酸平衡日粮对生长猪氮、磷及能量代谢的影响[J].中国畜牧杂志,2006,42(13):23-26.
[12] 刘源,呙于明,安星兰,等.成年比格犬蛋白质需要量研究[J].实验动物科学,2001,18(3):41-43.
[13] 淡瑞芳,张海涛,郑建婷,等.成年贵宾犬蛋白质需要量的研究(简报)[J].甘肃农业大学学报,2009,44(4):29-31,35.
[14] 周俊,韩向敏,张海涛,等.成年藏獒犬蛋白质需要量的研究[J].甘肃农业大学学报,2011,46(1):14-17.
[15] 国家学术委员会下属的国家研究委员会.犬猫营养需要[M].丁丽敏,夏兆飞,译.2版.北京:中国农业大学出版社,2017:31-52.
[16] AOAC.Official methods of analysis[S].Arlington:Association of Official Analytical Chemists,2003.
[17] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:68-70.
[18] CHEN H Y, LEWIS A J,MILLER P S,et al.The effect of excess protein on growth performance and protein metabolism of finishing barrows and gilts[J].Journal of Animal Science,2000,77(12):3238-3247.
[19] 李元晓,庞有志,周俊华,等.日粮蛋白质水平对黄羽鹌鹑饲料消化率及肠道菌群的影响[J].饲料工业,2010,31(3):54-57.
[20] 崔虎,张铁涛,张志强,等.饲粮蛋白质水平对冬毛期蓝狐生长性能、营养物质消化代谢及血清生化指标的影响[J].动物营养学报,2011,23(12):2217-2224.
[21] 朱元召,葛金山,胡忠泽,等.低蛋白质日粮预混料对猪生长和消化的影响[J].饲料研究,2012(2):1-3.
[22] 武江利.维生素E对育成鸭生产性能及机体生化指标的影响[D].硕士学位论文.哈尔滨:东北农业大学,2009.
[23] 姬真真,丁丽敏,付京杰.断奶至4月龄藏獒最适能量和蛋白质水平的研究[J].现代牧业,2017,1(1):39-43.
[24] 武江利,任延铭,王安,等.维生素E添加水平对笼养生长蛋鸭血清生化指标的影响[J].东北农业大学学报,2009,40(2):70-74.
[25] 李秋波,康纪平,冯晓科,等.Beagle犬正常血液学指标及血清生化指标测定[J].四川动物,2011,30(2):265-267.
[26] 刘运忠,龚宝勇,罗甜仪,等.Beagle犬的基础数据[J].中国比较医学杂志,2009,19(3):60-63.
[27] 梁明振,梁贤威,梁坤,等.益生素对断奶仔猪生产性能及血清相关指标的影响[J].动物科学与动物医学,2002,19(12):49-51.
[28] 刘伟,刘盛南,李俊营,等.不同营养水平日粮对蛋用鹌鹑产蛋性能及血清生化指标的影响[J].安徽农业科学,2014,42(25):8617-8618.
[29] 王宗伟,牟晓玲,杨国伟,等.日粮营养素水平对东北肉鹅生长性能及血液生化指标的影响(1-28日龄)[J].核农学报,2009,23(5):891-897.
[30] 吕继蓉,陈邦云,喻麟,等.日粮蛋白质水平及组成对动物免疫功能的影响[J].饲料工业,2009,30(6):42-44.
[31] 吕建敏,程鉴冰,屠珏,等.日粮粗蛋白质水平对生长期实验兔生长性能和免疫功能的影响[J].动物营养学报,2010,22(1):75-81.
[32] 贺绍君,丁金雪,李静,等.高温对犬血清急性期反应标志物及氧化还原状态的影响[J].营养学报,2018,40(5):488-492.
[33] JIA F X,DOU W,HU F,et al.Effects of thermal stress on lipid peroxidation and antioxidant enzyme activities of oriental fruit fly,Bactrocera dorsalis (Diptera:Tephritidae)[J].Florida Entomologist,2011,94(4):956-963.  
[34] 谢继青,李玉华,杨春梅,等.超氧化物歧化酶的药理作用[J].中国生化药物杂志,2009,30(1):72-75.
[35] 索帅,李健,李吉涛,等.蛋白质和脂肪水平对脊尾白虾生长、非特异性免疫力及抗病力的影响[J].中国渔业质量与标准,2015,5(4):40-47.
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