Effects of Folic Acid on Expression of Oxidative Stress-Related Proteins and Endoplasmic Reticulum Stress-Related Proteins in Lead-Induced Lung of Rats

  • LI Ning ,
  • ZHAO Yali ,
  • CHENG Yongxia ,
  • SHEN Yue ,
  • SONG Lianjun ,
  • HUANG Xianqing ,
  • QIAO Mingwu ,
  • CAO Shuai ,
  • ZHANG Di ,
  • ZHAO Ziwei ,
  • TAI Jiajia
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  • 1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China;
    2. Henan Engineering Research Center for Food Processing and Distribution Safety Control, Zhengzhou 450002, China

Received date: 2020-03-21

  Online published: 2020-09-17

Abstract

The purpose of this experiment was to study the effects of folic acid on the expression of oxidative stress-related proteins nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) and endoplasmic reticulum stress-related proteins C/EBP homologous protein (CHOP) and glucose-regulated protein 78 (GRP78) in lead-induced lung of rats, and to explore the intervention effect of folic acid on lung tissue damage caused by lead exposure. Thirty-two specific pathogen free (SPF)-grade adult SD rats (male) were averagely divided into 4 groups (n=8) according to body weight:control group (group C, free drinking water+1 mL deionized water gavage), lead group (group L, free drinking 0.2% lead acetate solution+1 mL deionized water gavage), intervention group (group I, free drinking 0.2% lead acetate solution+1 mL folic acid suspension gavage), and folic acid group F(group F, free drinking water+1 mL folic acid suspension gavage). The folic acid suspension was prepared according to the dose of 0.4 mg/kg BW folic acid. Rats in each group were given intragastric administration once a day. After 14 days, the lung tissue was sampled and the expression levels of Nrf2, HO-1, CHOP and GRP78 proteins were measured by immunofluorescence method. The results showed that compared with group C, groups L, I and F had no significant changes in initial body weight, final body weight, daily gain, net gain and lung index (P>0.05); the expression levels of CHOP and GRP78 proteins in groups L, I and F were significantly increased (P<0.05); the expression level of HO-1 protein in groups L and I was significantly increased (P<0.05), but the expression level of Nrf2 protein had no significant change (P>0.05); the expression level of HO-1 protein in group F was significantly decreased (P<0.05), but the expression level of Nrf2 protein was no significantly changed (P>0.05). Compared with group L, groups I and F had no significant changes in initial body weight, final body weight, daily gain, net gain and lung index (P>0.05); the expression levels of HO-1 and CHOP proteins in groups I and F were significantly reduced (P<0.05), but the expression levels of Nrf2 and GRP78 proteins had no significant changes (P>0.05). It can been seen from the above results:firstly, the expression levels of HO-1 and CHOP proteins are increased induced by lead exposure, but the expression levels of HO-1 and CHOP proteins are reduced after folic acid intervention; secondly, the oxidative stress caused by lead exposure is closely related to the Nrf2/HO-1 and GRP78/CHOP pathways, and folic acid directly affects the expression of HO-1 and CHOP proteins; thirdly, the dose of 0.4 mg/kg BW of folic acid has a certain antagonistic effect on oxidative stress and endoplasmic reticulum stress caused by 0.2% lead acetate exposure in rats with lung injury.

Key words: folic acid; lead; rats; lung; Nrf2; HO-1; CHOP; GRP78

Cite this article

LI Ning , ZHAO Yali , CHENG Yongxia , SHEN Yue , SONG Lianjun , HUANG Xianqing , QIAO Mingwu , CAO Shuai , ZHANG Di , ZHAO Ziwei , TAI Jiajia . Effects of Folic Acid on Expression of Oxidative Stress-Related Proteins and Endoplasmic Reticulum Stress-Related Proteins in Lead-Induced Lung of Rats[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4318 -4326 . DOI: 10.3969/j.issn.1006-267x.2020.09.042

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