Tannic Acid Induces Oxidative Damage and Apoptosis in Pig Kidney Cells

WANG Ji; ZHANG Linyu; LIU Xiangyan; XIAO Haisi; LI Xin; YAN Sisi; YUAN Zhihang; LIANG Zengenni; WEN Lixin; WU Jing

doi:10.3969/j.issn.1006-267x.2020.09.043

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 9: 4327 - 4336

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.043

Tannic Acid Induces Oxidative Damage and Apoptosis in Pig Kidney Cells

WANG Ji ,
ZHANG Linyu ,
LIU Xiangyan ,
XIAO Haisi ,
LI Xin ,
YAN Sisi ,
YUAN Zhihang ,
LIANG Zengenni ,
WEN Lixin ,
WU Jing

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1. Faculty of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;
2. Changsha Lvye Biotechnology Co., Ltd., Changsha 410100, China;
3. Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
4. Hunan Collaborative Innovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2020-03-19

Online published: 2020-09-17

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Abstract

The aim of this study was to explore the molecular mechanism of tannic acid (TA) mediated renal cytotoxicity in animals, and to provide experimental basis for the reduction and utilization of antinutritional factors in feed. Pig kidney cell line PK-15 cells were treated with different concentrations of TA[0 (blank control), 10, 20 and 30 μmol/L] for 24 h. Cell viability, lactate dehydrogenase (LDH) activity, oxidative damage parameters, mitochondrial membrane potential (MMP), apoptosis related protein expression and cell cycle were measured. The results showed that TA decreased the viability of PK-15 cells in a dose-dependent manner. Compared with the blank control, the treatment of 30 μmol/L TA extremely significantly increased the activity of LDH in the medium (P<0.01); extremely significantly increased malondialdehyde (MDA) content of PK-15 cells (P<0.01), significantly or extremely significantly reduced the content of glutathione (GSH) (P<0.01) and the activities of glutathione peroxidase (GSH-Px) (P<0.01), superoxide dismutase (SOD) (P<0.01) and catalase (CAT) (P<0.05), and extremely significantly increased the content of reactive oxygen species (ROS) (P<0.01); extremely significantly reduced the MMP of PK-15 cells (P<0.01). The treatment of 30 μmol/L TA could extremely significantly or significantly increase B cell lymphoma-2 (Bcl-2) associated X protein (Bax)/Bcl-2 (P<0.01), cleaved-Caspase-3/pro-Caspase-3 (P<0.01) and cleaved-Caspase-9/pro-Caspase-9 (P<0.05). In addition, the treatment of 30 μmol/L TA could induce the accumulation of PK-15 cells in S-phase, leading to S-phase arrest. It is concluded that high concentrations of TA induce oxidative damage and intracellular ROS-mediated mitochondrial pathway apoptosis in PK-15 cells, as well as leading to S-phase arrest of the cells.

Key words： tannic acid; PK-15 cells; oxidative damage; cell apoptosis; antinutritional factor

Cite this article

WANG Ji , ZHANG Linyu , LIU Xiangyan , XIAO Haisi , LI Xin , YAN Sisi , YUAN Zhihang , LIANG Zengenni , WEN Lixin , WU Jing . Tannic Acid Induces Oxidative Damage and Apoptosis in Pig Kidney Cells[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4327 -4336 . DOI: 10.3969/j.issn.1006-267x.2020.09.043

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