Effects of Tumor Necrosis Factor-α Stimulation on Viability, Physical Barrier and Inflammation-Related Gene Expression of IPEC-1 Cells

  • LI Pei ,
  • WANG Shuhui ,
  • CHEN Shaokui ,
  • WANG Xiuying ,
  • XIAO Kan ,
  • LIU Yulan ,
  • WANG Dan
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2020-03-16

  Online published: 2020-09-17

Abstract

This study was investigated the effects of tumor necrosis factor-α (TNF-α) stimulation on viability, lactate dehydrogenase (LDH) activity, tight junction protein and inflammation-related gene expression of IPEC-1 cells. Selected 0 (control group), 10, 20, 40 and 50 ng/mL of TNF-α to stimulate IPEC-1 cells for 12, 24 and 48 h, respectively, and detected relevant indexes. The results showed as follows:1) compared with the control group, different concentrations of TNF-α significantly decreased cell viability at stimulation for 24 h (P<0.05); TNF-α at 40 and 50 ng/mL resulted in a significant decrease in cell viability at stimulation for 48 h (P<0.05). 2) Compared with the control group at stimulation for 24 and 48 h, TNF-α at 40 and 50 ng/mL led to a significant increase in LDH activity (P<0.05). 3) Compared with the control group at stimulation for 24 h, TNF-α at 40 and 50 ng/mL significantly increased the mRNA expression levels of tight junction proteins zonula occluden-1 (ZO-1), Claudin-1 and Occludin of IPEC-1 cells (P<0.05); different concentrations of TNF-α had no significant effects on Claudin-1 protein expression level at stimulation for 48 h (P>0.05). 4) Compared with the control group at stimulation for 24 and 48 h, different concentrations of TNF-αsignificantly increased the mRNA expression levels of TNF-α and interleukin-8 (IL-8) (P<0.05), and in a time- and dose-dependent manner. The above results indicate that TNF-α stimulation induces inflammatory response and damage in IPEC-1 cells. By comprehensive consideration, the IPEC-1 cell damage model can be induced by 40 ng/mL TNF-α at stimulation for 48 h.

Cite this article

LI Pei , WANG Shuhui , CHEN Shaokui , WANG Xiuying , XIAO Kan , LIU Yulan , WANG Dan . Effects of Tumor Necrosis Factor-α Stimulation on Viability, Physical Barrier and Inflammation-Related Gene Expression of IPEC-1 Cells[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4337 -4344 . DOI: 10.3969/j.issn.1006-267x.2020.09.044

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