Effects of Astragalus Polysaccharides on Morphology and Immune Function of Lipopolysaccharide-Stimulated Macrophages in Mice

  • CHEN Guangyong ,
  • HAN Qianjie ,
  • ZHANG Lingling ,
  • JIN Qingri ,
  • CUI Yanjun ,
  • YANG Caimei
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  • 1. College of Animal Science and Technology·College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China;
    2. Zhejiang Vegamax Biotech Co., Ltd., Anji 311703, China

Received date: 2020-03-16

  Online published: 2020-09-17

Abstract

The purpose of this study was to investigate the effects of Astragalus polysaccharides (APS) on the morphology and immune function of lipopolysaccharide (LPS)-stimulated macrophages in mice. LPS was used to stimulate mouse macrophages (RAW264.7 cells), and the effects of different concentrations (0.1, 0.5 and 1.0 mg/mL) of APS on the regulation of cellular immune stress caused by LPS stimulation were studied. The results showed as follows:1) 1.0 mg/mL APS can inhibit the changes of cell morphology and proliferation induced by LPS stimulation. 2) 1.0 mg/mL APS could significantly increase the activity of macrophage acid phosphatase (P<0.05), and 0.1, 0.5 and 1.0 mg/mL APS could significantly alleviate the reduction in alkaline phosphatase activity induced by LPS stimulation (P<0.05). 3) The increases of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents induced by LPS were significantly decreased by different concentrations of APS (P<0.05). 4) LPS stimulation significantly increased the mRNA expression levels of macrophages Toll-like receptor 4 (TLR4), myeloid differential factor 88 (MyD88) and nuclear factor-κB (NF-κB) (P<0.05), while the mRNA expression levels of macrophages TLR4, MyD88 and NF-κB significantly decreased after the addition of 1 mg/mL APS (P<0.05). In summary, APS can improve the macrophage morphology and restore cell proliferation ability of mice. By regulating the expression of genes related TLR4/NF-κB signaling pathway, the secretion of pro-inflammatory cytokines IL-1β and TNF-α are decreased to reduce the body's immune stress response.

Cite this article

CHEN Guangyong , HAN Qianjie , ZHANG Lingling , JIN Qingri , CUI Yanjun , YANG Caimei . Effects of Astragalus Polysaccharides on Morphology and Immune Function of Lipopolysaccharide-Stimulated Macrophages in Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4358 -4365 . DOI: 10.3969/j.issn.1006-267x.2020.09.046

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