Effects of Sampling Procedure on Determination of Residual Rate of Phytase Activity in Steam Conditioning-Pelleting Process

WU Yuxuan; ZHAO Feng; SU Yanfang; ZHANG Hu; LI Zhefeng; ZHANG Qianyun; ZHAO Wei; HE Xi

doi:10.3969/j.issn.1006-267x.2020.09.047

2020 , Vol. 32 >Issue 9: 4366 - 4375

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.047

Effects of Sampling Procedure on Determination of Residual Rate of Phytase Activity in Steam Conditioning-Pelleting Process

WU Yuxuan ,
ZHAO Feng ,
SU Yanfang ,
ZHANG Hu ,
LI Zhefeng ,
ZHANG Qianyun ,
ZHAO Wei ,
HE Xi

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science, Hunan Agricultural University, Changsha 410128, China;
3. Provincial Key Agricultural Enterprise Research Institute of Encapsulated Feed Additive, King Techina Technology Co., Ltd., Hangzhou 311107, China

Received date: 2020-03-27

Online published: 2020-09-17

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Abstract

The objective of this study was to investigate the effects of variation in phytase activity of samples collected from different time zones in steam conditioning-pelleting on the determination of residual rate of phytase. A complete random design was adopted. When the steam conditioning-pelleting was stable, samples were collected for 1 min every 2 min at 4 sites of anterior conditioning, posterior conditioning, pelleting or cooling. Thirty samples were collected at each sampling site. A sampling time zone was defined with 10 samples continuously collected within 20 min, thus there were 21 sampling time zones (such as 0 to 18 min, 2 to 20 min). The phytase activity was determined for each sample collected from each site in steam conditioning-pelleting. The residual rates of phytase activity were calculated for each sampling time zone. The average phytase activity in the representative sampling time zones was compared with determined phytase activity of the pooled 10 samples. The results showed that in the sampling time zone of 0 to 58 min after the conditioning temperature was stable at 75℃, the phytase activity of diets decreased from 39.8, 28.3, 20.1 to 14.7 U/g in sequence along the conditioning-pelleting-cooling process. The phytase activity of diets fluctuated greatly after conditioning and cooling (CV ≥ 21.9%). In 3 non-overlapping sample time zones of 0 to 18 min, 20 to 38 min and 40 to 58 min, the average phytase activity of samples collected from 0 to 18 min was significantly lower than that of samples collected from 20 to 38 min after conditioning (P<0.05). The average phytase activities of samples collected from 0 to 18 min or 20 to 38 min after conditioning-pelleting-cooling were significantly greater than that of samples collected from 40 to 58 min (P<0.05). In the sampling time zones of 12 to 30 min, 14 to 32 min, 16 to 34 min, and 18 to 36 min, the average phytase activity and residual rate of phytase activity were close to the average value of full samples. In each sampling time zones of 14 to 32 min, 16 to 34 min and 18 to 36 min, significant differences were observed between average phytase activity in 10 samples and determined values of that in pooled 10 samples from some collection sites (P<0.05), but the relative differences were less than 8%. In conclusion, after the conditioning temperature is stable for 12 to 18 min, the average phytase activity of 10 samples collected for 1 min every 2 min can represent the mean value of full diet samples in the steam condition-pelleting process.

Key words： conditioning-pelleting; sampling; phytase; residual rate

Cite this article

WU Yuxuan , ZHAO Feng , SU Yanfang , ZHANG Hu , LI Zhefeng , ZHANG Qianyun , ZHAO Wei , HE Xi . Effects of Sampling Procedure on Determination of Residual Rate of Phytase Activity in Steam Conditioning-Pelleting Process[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4366 -4375 . DOI: 10.3969/j.issn.1006-267x.2020.09.047

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