Repairing Effects of Duck Oil Diglyceride Combined with Vitamin K1 on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

CONG Hongxia; WANG Baowei; GE Wenhua; ZHANG Ming'ai; WANG Qian; XU Huixin; FAN Wenlei; KONG Min

doi:10.3969/j.issn.1006-267x.2020.09.048

2020 , Vol. 32 >Issue 9: 4376 - 4385

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.048

Repairing Effects of Duck Oil Diglyceride Combined with Vitamin K₁ on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

CONG Hongxia ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming'ai ,
WANG Qian ,
XU Huixin ,
FAN Wenlei ,
KONG Min

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1. Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2020-03-14

Online published: 2020-09-17

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Abstract

This experiment was conducted to study the repairing effects of duck oil diglyceride (DDG) combined with vitamin K₁ on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) injury in mice. The experiment was divided into two phases. Modeling phase:a total of 250 mice were randomly divided into 2 groups, the control group contained 5 replicates per group and 10 mice per replicate, and the inflammatory model group contained 20 replicates per group and 10 mice per replicate. Mice in the control group were free to drink distilled water, and the others in inflammatory model group were free to drink 3% DSS aqueous solution to induce the intestinal inflammation of mice, which lasted for 7 days. Repair intervention phase:a total of 160 mice were randomly divided into control group (group Ⅰ), inflammatory model group (group Ⅱ) and repair groups (groups Ⅲ to Ⅷ), and each group contained 4 replicates and 5 mice per replicate. Mice in groups Ⅰ and Ⅱ were gavage with distilled water (5.00 mL/kg BW); mice in groups Ⅲ to Ⅷ were gavage with different levels of DDG and vitamin K₁, and the DDG supplemental levels were 52.50, 3.75 and 5.00 mL/kg BW, respectively; and the vitamin K₁ supplemental levels were 1.00 and 2.00 mg/kg BW, respectively. The experiment lasted for 7 days. The results showed as follows:1) the body weight after modeling and colonic length of mice of inflammatory model group were significantly lower than those of the control group (P<0.05), and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in serum were significantly higher than those of the control group (P<0.05), it showed that the ulcerative colitis mice model successful build. 2) The body weight after repairing and colonic length of groups Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ and Ⅷ were significantly higher than those of group Ⅱ (P<0.05), the serum myeloperoxidase (MPO) activity of group Ⅳ was gnificantly lower than that of groups Ⅱ, Ⅲ, Ⅶ and Ⅷ (P<0.05). DDG combined with vitamin K₁ had a significant interaction on serum MPO activity of UC mice (P<0.05). 3) DDG combined with vitamin K₁ had significant interaction on serum inflammatory cytokine (IL-1β, IL-4, IL-6, IL-17, TNF-α and IL-10) contents of UC mice (P<0.05). 4) The colonic villus height of groups Ⅳ and Ⅵ was gnificantly higher than that of groups Ⅱ, Ⅲ, Ⅶ and Ⅷ (P<0.05), the colonic crypt depth of groups Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ and Ⅷ was gnificantly lower than that of group Ⅱ (P<0.05), and the colonic villus height/crypt depth of group Ⅵ was gnificantly higher than that of groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅶ and Ⅷ (P<0.05). DDG combined with vitamin K₁ had significant interaction on colonic villus height and villus height/crypt depth of UC mice (P<0.05). 5) The liver vitamin K₁ content of group Ⅷ was gnificantly higher than that of groups Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ (P<0.05). DDG combined with vitamin K₁ had significant interaction on liver vitamin K₁ content of UC mice (P<0.05). In conclusion, DDG combined with vitamin K₁ can effectively reduced the release of inflammatory cytokines, promote the repair and development of intestinal tissues; DDG can promote the absorption of vitamin K₁ in liver of UC mice, accelerate the hemostasis and repair of enteritis. The mice gavage with 3.75 mL/kg BW DDG and 1.00 mg/kg BW vitamin K₁ have the best repair effect on UC injury.

Key words： duck oil diacylglycerol; vitamin K₁; ulcerative colitis; repair

Cite this article

CONG Hongxia , WANG Baowei , GE Wenhua , ZHANG Ming'ai , WANG Qian , XU Huixin , FAN Wenlei , KONG Min . Repairing Effects of Duck Oil Diglyceride Combined with Vitamin K₁ on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4376 -4385 . DOI: 10.3969/j.issn.1006-267x.2020.09.048

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