Effects of Jujube Polysaccharide on Proliferation of Mouse Spleen B Lymphocytes in Vitro

GUO Linxia; LI Shupeng; MA Kewei; HAO Yanshuang; ZHAO Guoxian; YANG Bowen; ZHENG Ziwei; ZHANG Da; ZHANG Sixuan; FENG Zhihua; LIU Guanzhong

doi:10.3969/j.issn.1006-267x.2020.09.049

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 9: 4386 - 4392

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.049

Effects of Jujube Polysaccharide on Proliferation of Mouse Spleen B Lymphocytes in Vitro

GUO Linxia ,
LI Shupeng ,
MA Kewei ,
HAO Yanshuang ,
ZHAO Guoxian ,
YANG Bowen ,
ZHENG Ziwei ,
ZHANG Da ,
ZHANG Sixuan ,
FENG Zhihua ,
LIU Guanzhong

Expand

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
2. Baoding Vocational and Technical College, Baoding 071000, China

Received date: 2020-03-14

Online published: 2020-09-17

Fold

Abstract

The purpose of this experiment was to explore the effects of jujube polysaccharide (JP) extracted with different ethanol concantrations and cooperated with lipopolysaccharide (LPS) on the proliferation of mouse spleen B lymphocytes in vitro. In this experiment, four jujube polysaccharides were obtained by water extraction and alcohol precipitation method and step-by-step alcohol precipitation method in turn. Mouse spleen B lymphocytes were sterilely separated by mechanical method. The cells were adjusted to 5×10⁴ cells/mL and randomly divided into 18 groups with 4 replicates in each group and 85 μL cells in each replicate. The experiment adopted a 4×4 two-factor experimental design. The concentrations of ethanol and JP were two main effects. The concentration of ethanol were 60%, 70%, 80% and 90%, respectively, and the concentrations of JP were 25, 50, 100 and 150 μg/mL, respectively. In addition, one group of blank control (without addition of JP and LPS) and one group of LPS control (without addition of JP and only addition of LPS) were set up. The proliferation of B lymphocytes in each group was detected by MTT method, and the relative proliferation rate was calculated. Results showed as follows:1) the relative proliferation rate of spleen B lymphocytes increased with the increase of ethanol concentration, the relative proliferation rate of the 90% ethanol concentration group was significantly higher than that of the 60%, 70% and 80% ethanol concentration groups (P<0.05), the relative proliferation rate of the 70% and 80% ethanol concentration groups was significantly higher than that of the 60% ethanol concentration group (P<0.05), and there was no significant difference between the 70% and 80% ethanol concentration groups (P>0.05). 2) With the increase of JP concentration, the relative proliferation rate first increased and then decreased. All the test groups were significantly higher than LPS control group and blank control group (P<0.05), and the differences among the test groups were significant (P<0.05). When JP concentration was 100 μg/mL, the relative proliferation rate was the largest. 3) The interaction of ethanol concentration and JP concentration had a significant effect on the relative proliferation rate of mouse spleen B lymphocytes (P<0.05). The relative proliferation rate of LPS control group and each test groups was significantly higher than that of blank control group (P<0.05). The relative proliferation rate of 60% ethanol concentration and 25 μg/mL JP concentration group was significantly lower than that of LPS control group (P<0.05), while that of other groups was significantly higher than that of LPS control group (P<0.05). Under the condition of in vitro culture, the relative proliferation rate of mouse spleen B lymphocytes increases with the increase of ethanol concentration, and shows a "bell-jar" change with the increase of JP concentration, with a saturation concentration of 100 μg/mL. The interaction of JP concentration and ethanol concentration can significantly promote the proliferation of spleen B lymphocytes in mice, and the best effect is obtained when the ethanol concentration is 80% and JP concentration is 100 μg/mL.

Key words： jujube polysaccharide; splenic B lymphocyte; lipopolysaccharide; relative proliferation rate

Cite this article

GUO Linxia , LI Shupeng , MA Kewei , HAO Yanshuang , ZHAO Guoxian , YANG Bowen , ZHENG Ziwei , ZHANG Da , ZHANG Sixuan , FENG Zhihua , LIU Guanzhong . Effects of Jujube Polysaccharide on Proliferation of Mouse Spleen B Lymphocytes in Vitro[J]. Chinese Journal of Animal Nutrition, 2020 , 32(9) : 4386 -4392 . DOI: 10.3969/j.issn.1006-267x.2020.09.049

References

[1] 陈代文,吴德.饲料添加剂学[M].2版.北京:中国农业出版社,2011:76-81.
[2] 王留,杨艳玲.大枣多糖对肉仔鸡生长性能和免疫功能的影响[J].饲料工业,2012,33(6):8-9.
[3] 张庆,雷林生,林勒保,等.大枣多糖体外抗补体活性及促进小鼠脾细胞增殖作用[J].中药药理与临床,1998,14(5):19-21.
[4] 张庆,雷林生,杨淑琴,等.大枣中性多糖对小鼠腹腔巨噬细胞分泌肿瘤坏死因子及其mRNA表达的影响[J].第一军医大学学报,2001,21(8):592-594.
[5] 赵耀光,王留.大枣多糖对蛋雏鸡生长性能和免疫功能的影响[J].饲料研究,2012(7):79-80.
[6] JI X L,HOU C Y,YAN Y Z,et al.Comparison of structural characterization and antioxidant activity of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit[J].International Journal of Biological Macromolecules,2020,149:1008-1018.
[7] 蔡冰洁,汪苗苗,刘咏.枳椇多糖的分级醇沉及其免疫调节活性的研究[J].合肥工业大学学报(自然科学版),2018,41(6):852-858.
[8] 胡伟莲,戴德慧.红参药渣中多糖的提取及其醇沉性质分析[J].食品研究与开发,2015,36(4):58-61.
[9] WILLERSHAUSEN B,MARROQUIN B B,SCHAFER D,et al.Cytotoxicity of root canal filling materials to three different human cell lines[J].Journal of Endodontics,2000,26(12):703-707.

[10] SHINCHI H,CRAIN B,YAO S Y,et al.Enhancement of the immunostimulatory activity of a TLR7 ligand by conjugation to polysaccharides[J].Bioconjugate Chemistry,2015,26(8):1713-1723.

[11] TUVAANJAV S,SHUQIN H,KOMATA M,at al.Isolation and antiviral activity of water-soluble Cynomorium songaricum Rupr. polysaccharides[J].Journal of Asian Natural Products Research,2016,18(2):159-171.

[12] 王晓利,廖成水,程源斌,等.甘草甜素甘草多糖和光甘草定对小鼠巨噬细胞的毒性与免疫功能的调节[J].中国兽医科学,2014,44(3):320-325.
[13] ZHU Z Y,LIU N,SI C L,et al.Structure and anti-tumor activity of a high-molecular-weight polysaccharide from cultured mycelium of Cordyceps gunnii[J].Carbohydrate Polymers,2012,88(3):1072-1076.

[14] 刘泽华.大枣多糖提取纯化工艺及其生物活性研究进展[J].农业科技与装备,2016(10):62-63.
[15] 赵骏,方玲,于坤路,等.桑叶多糖不同分子量段降血糖作用研究[J].中药材,2010,33(1):108-110.
[16] 李赞阳,刘红,李炳奇,等.不同组分、不同纯度中药复方多糖对小鼠脾淋巴细胞增殖影响[J].中兽医医药杂志,2011,30(2):5-7.
[17] 王忠,马儒林,牛强,等.沙枣多糖对小鼠免疫功能的影响[J].现代预防医学,2013,40(2):222-224.
[18] 叶文斌,樊亮,王昱,等.拐枣多糖对环磷酰胺诱导免疫低下小鼠免疫功能的影响[J].现代食品科技,2016,32(7):26-32.
[19] 袁红波,赵文,赵仁邦,等.金丝小枣糖浆对小鼠免疫功能的影响[J].中国食品学报,2008,8(1):13-16.
[20] 赵仁,刘孟军,葛微,等.高效液相色谱法测定枣中的糖类物质[J].食品科学,2004,25(8):138-142.
[21] 杨贵贞.医用免疫学[M].长春:吉林人民出版社,1981.
[22] 郑乃珍,郑小香,李萍,等.猴头菇多糖对小鼠脾淋巴细胞体外增殖及细胞周期的影响[J].中国兽医科学,2015,45(8):837-842.
[23] 张陆曦,徐红丽,顾佳雯,等.条斑紫菜多糖PY-D2对小鼠脾淋巴细胞生长的影响[J].中国免疫学杂志,2007,23(12):1093-1096.
[24] 赵婷婷.不同分子量坛紫菜多糖的制备及其抗衰老活性研究[D].博士学位论文.青岛:中国科学院研究生院(海洋研究所),2007.
[25] SHAO B M,XU W,DAI H,et al.A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus,a Chinese medicinal herb[J].Biochemical and Biophysical Research Communications,2004,320(4):1103-1111.

[26] 李晓亮,魏娜,王鹏程,等.商陆均一多糖对小鼠脾细胞增殖及细胞因子分泌的影响[J].中医药学报,2019,47(3):10-13.
[27] 蔡雨晴.金昌枣多糖提取分离、结构表征及生物活性的研究[D].硕士学位论文.阿拉尔:塔里木大学,2018.
[28] 魏然.圆铃大枣多糖提取、纯化及生物活性研究[D].硕士学位论文.泰安:山东农业大学,2014.
[29] 朱轶锋,韩顺顺,张克英,等.黄芪多糖对鸡淋巴细胞体外增殖及脾脏淋巴细胞分泌细胞因子和相关mRNA表达的影响[J].四川农业大学学报,2018,36(5):674-680.
[30] 李志涛,孙金旭,刘海鹏,等.蛹虫草多糖的提取及对小鼠脾细胞增殖的影响[J].食品研究与开发,2020,41(2):117-123.
[31] 张庆,雷林生,杨淑琴,等.大枣中性多糖对小鼠脾淋巴细胞增殖的影响[J].第一军医大学学报,2001,21(6):426-428.
[32] 张红英,王亚宾,王学兵,等.板蓝根多糖对体外培养的猪脾脏淋巴细胞增殖及分泌细胞因子和NO的影响[J].河南农业大学学报,2009,43(2):173-176.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References