Effects of Bacillus subtilis on Growth Performance, Nutrient Apparent Digestibility and Intestinal Flora of Weaned Donkey Foals

ZHANG Ting; FENG Peixiang; WANG Zhuo; LIU Xiaoying; SI Huazhe; JIANG Guimiao; ZHAO Fuwei; LI Guangyu; LIU Hanlu

doi:10.3969/j.issn.1006-267x.2020.10.046

2020 , Vol. 32 >Issue 10: 4956 - 4962

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.10.046

Effects of Bacillus subtilis on Growth Performance, Nutrient Apparent Digestibility and Intestinal Flora of Weaned Donkey Foals

ZHANG Ting ,
FENG Peixiang ,
WANG Zhuo ,
LIU Xiaoying ,
SI Huazhe ,
JIANG Guimiao ,
ZHAO Fuwei ,
LI Guangyu ,
LIU Hanlu

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1. Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Science, Changchun 130112, China;
2. Engineering Research Center of Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Dong'e 252201, China

Received date: 2020-04-10

Online published: 2020-10-22

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Abstract

This experiment was conducted to study the effects of Bacillus subtilis on growth performance, nutrient apparent digestibility and intestinal flora of weaned donkey foals. Ten 5 to 6-month-old female weaned donkey foals with an average body weight of (113.40±17.52) kg, were randomly divided into 2 groups with 5 replicates per group and 1 donkey per replicate, and they were fed diets: a basal diet (A group) and the basal diet supplemented with Bacillus subtilis (B group, the addition amount of Bacillus subtilis in the concentrate was 10¹¹ CFU/kg, and the concentrate was fed based on the body weight of 1.5%). The results showed that Bacillus subtilis supplementation significantly increased the average body weight gain of weaned donkey foals from days 1 to 30 (P<0.05). There was no significant difference in growth performance between groups from days 31 to 60 (P>0.05). Diets supplemented with Bacillus subtilis significantly increased the ether extract apparent digestibility (P<0.05), however, there was no significant difference in the apparent digestibility of dry mater, crud protein, neutral detergent fiber and acid detergent fiber (P>0.05). The relative abundance of Bacteroidetes of group B was significantly higher than that of group A (P<0.05), and there was no significant difference between the two groups at the bacterial genus level (P>0.05). However, the two donkey foals with high relative abundance of Proteobacteria had higher relative abundance of Acinetobacter of group A, reaching 34.35% to 48.12%. Overall, Bacillus subtilis supplementation can improve daily body weight gain, ether extract apparent digestibility and Bacteroidetes relative abundance of donkey foals at the early weaning stage, and has a certain probiotic effect on the growth performance of donkey foals.

Key words： weaned donkey foals; Bacillus subtilis; growth performance; nutrient apparent digestibility; intestinal flora

Cite this article

ZHANG Ting , FENG Peixiang , WANG Zhuo , LIU Xiaoying , SI Huazhe , JIANG Guimiao , ZHAO Fuwei , LI Guangyu , LIU Hanlu . Effects of Bacillus subtilis on Growth Performance, Nutrient Apparent Digestibility and Intestinal Flora of Weaned Donkey Foals[J]. Chinese Journal of Animal Nutrition, 2020 , 32(10) : 4956 -4962 . DOI: 10.3969/j.issn.1006-267x.2020.10.046

References

[1] 李沐森,郭文场,刘佳贺,等.中国驴的起源、类别、特征特性及品种简介[J].特种经济动植物,2019,22(1):2-7.
[2] 王仁虎.引起驴驹腹泻的致病性大肠杆菌分离鉴定及复合疫苗研制[D].硕士学位论文.聊城:聊城大学,2018.
[3] BAE S R,PARK C,CHOI J C,et al.Effects of ultra high molecular weight poly-gamma-glutamic acid from Bacillus subtilis (chungkookjang) on corneal wound healing[J].Journal of Microbiology & Biotechnology,2010,20(4):803-808.
[4] 邢帅兵,陈代文,余冰,等.枯草芽孢杆菌对断奶仔猪生长性能和肠道形态、黏膜免疫及菌群数量的影响[J].动物营养学报,2020,32(5):2066-2073.
[5] 苏勇华,方雷,应璐,等.枯草芽孢杆菌对多浪羊消化率、瘤胃发酵参数及血液指标的影响[J].江苏农业科学,2018,46(8):162-166.
[6] 彭豫东,康克浪,曲湘勇,等.枯草芽孢杆菌对石门土鸡生长性能、屠宰性能、血清抗氧化指标和肠道形态的影响[J].动物营养学报,2019,31(5):2119-2126.
[7] 李靖.枯草芽孢杆菌对荷斯坦育成牛生长性能和瘤胃微生物区系的影响[D].硕士学位论文.泰安:山东农业大学,2019.
[8] LI Z P,WRIGHT A D G,LIU H L,et al.Bacterial community composition and fermentation patterns in the rumen of sika deer (Cervus nippon) fed three different diets[J].Microbial Ecology,2015,69(2):307-318.

[9] 薛云,周锋,王乐伟.枯草芽孢杆菌在断奶仔兔生长和预防腹泻中的应用研究[J].中国饲料,2018(14):84-87.
[10] 郑雪,刘建博,宋百军,等.枯草芽孢杆菌对花马杂交F1代断奶仔鹿生长性能和血清生化指标的影响[J].黑龙江畜牧兽医,2015(11):230-232.
[11] KHAMPA S,CHAOWARAT P,CHUMPAWADEE S,et al.Effects of malate and cassava hay in high-quality feed block on ruminal fermentation efficiency and digestibility of nutrients in dairy steers[J].Asian Journal of Animal Sciences,2009,3(1):33-38.
[12] LIU Y F,CHEN Z Y,NG T B,et al.Bacisubin,an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916[J].Peptides,2007,28(3):553-559.

[13] 张海涛.纳豆枯草芽孢杆菌对犊牛生长发育以及瘤胃组织形态学发育的影响[D].硕士学位论文.北京:中国农业科学院,2009.
[14] 黄雪泉.添加枯草芽孢杆菌制剂对仔猪生产性能的影响[J].中国畜牧兽医,2010,37(7):212-214.
[15] BOOTH J A,MILLER-AUWERDA P A,RASMUSSEN M A.The effect of horse-Bac liquid on nutrient digestibility,gut physiology,and the physical response to exercise in horses[C]//Proceedings of the 17th Equine Nutr Physiol Soc Symp.[s.l]:[s.n.],2001:259-260.
[16] 郭梦娇.枯草芽孢杆菌的筛选及其诱导兔抗大肠杆菌感染先天性免疫应答机制研究[D].硕士学位论文.泰安:山东农业大学,2018.
[17] 陈丽梅,徐岳松,陈峰.枯草芽孢杆菌YK-1R对断奶仔猪肠道菌群的影响[J].基因组学与应用生物学,2018,37(9):3852-3858.
[18] VEPACHEDU V R,SETLOW P.Localization of SpoVAD to the inner membrane of spores of Bacillus subtilis[J].Journal of Bacteriology,2005,187(16):5677-5682.

[19] 陈茂金.南昌地区圈养野生动物肠道微生物多样性分析[D].硕士学位论文.南昌:江西农业大学,2018.
[20] 朱沛霁.枯草芽孢杆菌对雪山鸡生产性能、肠道健康和免疫机能的影响及机制[D].博士学位论文.扬州:扬州大学,2017.
[21] SPENCE C,WELLS W G,SMITH C J.Characterization of the primary starch utilization operon in the obligate anaero Bebacteroides fragilis:regulation by carbon source and oxygen[J].Journal of Bacteriology,2006,188(13):4663-4672.

[22] SHIN N R,WHON T W,BAE J W.Proteobacteria:microbial signature of dysbiosis in gut microbiota[J].Trends in Biotechnology,2015,33(9):496-503.

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