Developmental Changes of Expression of Tight Junction Protein Genes in Broiler Intestine

WEN Xueting; XIAO Yingping; LYU Wentao; LIU Xiuting; YANG Caimei; WANG Yongxia; YANG Hua

doi:10.3969/j.issn.1006-267x.2020.10.047

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 10: 4963 - 4971

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.10.047

Developmental Changes of Expression of Tight Junction Protein Genes in Broiler Intestine

WEN Xueting ,
XIAO Yingping ,
LYU Wentao ,
LIU Xiuting ,
YANG Caimei ,
WANG Yongxia ,
YANG Hua

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1. College of Animal Science & Technology, Zhejiang A&F University, Hangzhou 311300, China;
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Hangzhou 310021, China;
3. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2020-07-07

Online published: 2020-10-22

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Abstract

This experiment was conducted to investigate the developmental changes of expression of tight junction protein genes in different intestinal segments of broilers. The duodenal, jejunal, ileal, cecal and colonic tissue segments and mucous membrane were collected from 6 out of 60 fresh-hatched Ross 308 broilers at 1, 3, 7, 14 and 28 days of age, respectively. The relative expression levels of tight junction protein genes including Claudin-1, Claudin-2, Occludin, zonula occluden (ZO)-1 and ZO-2 genes in each intestinal segment were detected by real-time fluorescent quantitative (RT-qPCR) technique. The results were as follows: 1) at 1 day of age, the relative expression levels of Claudin-1 gene in the duodenum, jejunum, ileum and cecum were significantly higher than that at other days of age (P<0.05) whereas the relative expression level of Claudin-2 gene in all intestinal segment was the lowest (P<0.05). The relative expression level of Claudin-2 in the cecum and colon was increased with age increasing and reached the highest level at 7 days of age. The relative expression level of Claudin-2 gene decreased from duodenum to colon. 2) The relative expression level of Occludin gene in the duodenum, jejunum, ileum and colon at 1 day of age was significantly lower than that at other days of age (P<0.05), while it in the duodenum, jejunum and ileum was increased with age increasing, and reached a peak at 14 days of age. 3) The relative expression level of ZO-1 gene in all intestinal segments at 1 day of age was significantly lower than that at other days of age (P<0.05). The relative expression level of ZO-2 gene in the duodenum and jejunum was not significantly different with age increasing (P>0.05), while it in the ileum was increased with age increasing, reached a peak at 14 days of age and then decreased. 4) Both age and intestinal segment had significant effects on the relative expression levels of tight junction protein genes (P<0.05), and the interaction effects of age and intestinal segment on the relative expression levels of tight junction protein genes were also significant (P<0.01). In conclusion, the relative expression level of Claudin-1 gene is the highest at 1 day of age, whereas Claudin-2, Occludin and ZO-1 genes are the opposite. The intestinal permeability of broilers is the lowest at 1 day of age and then increase. The relative expression level of Claudin-2 gene decreases gradually from duodenum to colon, and the permeability of anterior intestine (duodenum, jejunum and ileum) is higher than that of posterior intestine (cecum and colon). The developmental patterns of different tight junction proteins are different from each other, the interaction effects of age and intestinal segment on the relative expression levels of tight junction protein genes are significant.

Key words： broilers; intestine; tight junction protein; developmental changes

Cite this article

WEN Xueting , XIAO Yingping , LYU Wentao , LIU Xiuting , YANG Caimei , WANG Yongxia , YANG Hua . Developmental Changes of Expression of Tight Junction Protein Genes in Broiler Intestine[J]. Chinese Journal of Animal Nutrition, 2020 , 32(10) : 4963 -4971 . DOI: 10.3969/j.issn.1006-267x.2020.10.047

References

[1] ANDERSON R C,DALZIEL J E,GOPAL P K,et al.The role of intestinal barrier function in early life in the development of colitis[J/OL].InTech,2012,doi:10.5772/25753.
[2] 陈继发,曲湘勇.家禽肠道屏障功能及其影响因素[J].广东饲料,2018,27(6):41-43.
[3] MADARA J L. Intestinal absorptive cell tight junctions are linked to cytoskeleton[J].American Journal of Physiology:Cell Physiology,2005,253(1):C171-C175.
[4] DOKLADNY K,ZUHL M N,MOSELEY P L.Intestinal epithelial barrier function and tight junction proteins with heat and exercise[J].Journal of Applied Physiology,2016,120(6):692-701.

[5] GONZÁLEZ-MARISCAL L,MIRANDA J,RAYA-SANDINO A,et al.ZO-2,a tight junction protein involved in gene expression,proliferation,apoptosis,and cell size regulation[J].Annals of the New York Academy of Sciences,2017,1397(1):35-53.

[6] SUZUKI T.Regulation of the intestinal barrier by nutrients:the role of tight junctions[J].Animal Science Journa,2020,91(1):e13357.
[7] CAPALDO C T,POWELL D N,KALMAN D.Layered defense:how mucus and tight junctions seal the intestinal barrier[J].Journal of Molecular Medicine,2017,95(9):927-934.

[8] 邢晓辉,李力仙,郭天林,等.Occludin蛋白与细胞间紧密连接关系及其临床意义[J].现代生物医学进展,2015,15(8):1553-1555,1405.
[9] 甘元涛.肠黏膜屏障损伤与紧密连接蛋白ZO-1的关系研究进展[J].现代医药卫生,2016,32(7):1027-1029.
[10] SCHNEIDERS G H,FOUTZ J C,MILFORT M C,et al.Heat stress reduces sexual development and affects pathogenesis of Eimeria maxima in meat-type chickens[J].Scientific Reports,2020,10(1):10736.
[11] BROOM L J.Gut barrier function:effects of (antibiotic) growth promoters on key barrier components and associations with growth performance[J].Poultry Science,2018,97(5):1572-1578.

[12] MORAN E T JR.Nutrients central to maintaining intestinal absorptive efficiency and barrier integrity with fowl[J].Poultry Science,2017,96(5):1348-1363.

[13] 马军宏,于向阳,张楠,等.紧密连接蛋白与肠黏膜屏障损伤研究进展[J].中国中西医结合外科杂志,2015,21(1):104-105.
[14] 马怡然,尚德淑,方文刚,等.细胞紧密连接的结构组成及其调控的研究进展[J].解剖科学进展,2010,16(1):71-74.
[15] MERGA Y,CAMPBELL B J,RHODES J M.Mucosal barrier,bacteria and inflammatory bowel disease:possibilities for therapy[J].Digestive Diseases,2014,32(4):475-483.

[16] DE MEDINA F S,ROMERO-CALVO I,MASCARAQUE C,et al.Intestinal inflammation and mucosal barrier function[J].Inflammatory Bowel Diseases,2014,20(12):2394-2404.

[17] ROSENTHAL R,MILATZ S,KRUG S M,et al.Claudin-2,a component of the tight junction,forms a paracellular water channel[J].Journal of Cell Science,2010,123(11):1913-1921.

[18] CHANG C H,TENG P Y,LEE T T,et al.Effects of multi-strain probiotic supplementation on intestinal microbiota,tight junctions,and inflammation in young broiler chickens challenged with Salmonella enterica subsp. enterica[J].Asian-Australasian Journal of Animal Sciences,2019,doi:10.5713/ajas.19.0427.
[19] ZHOU Q Q,COSTINEAN S,CROCE C M,et al.MicroRNA 29 targets nuclear factor-κB-repressing factor and Claudin 1 to increase intestinal permeability[J].Gastroenterology,2015,148(1):158-169.e8.

[20] ZHU L G,HAN J,LI L,et al.Claudin family participates in the pathogenesis of inflammatory bowel diseases and colitis-associated colorectal cancer[J].Frontiers in Immunology,2019,10:1441.
[21] ROBERTS S,PEREZ-GARCIA M,NEAL M,et al.Development of the small intestinal epithelial barrier function in broiler[J].Poultry Science,2005,84:74-75.
[22] ZHAO X N,YANG J,JU Z J,et al.Clostridium butyricum ameliorates Salmonella enteritis induced inflammation by enhancing and improving immunity of the intestinal epithelial barrier at the intestinal mucosal level[J].Frontiers in Microbiology,2020,11:299.
[23] SATO J,KANAZAWA A,WATADA H.Type 2 diabetes and bacteremia[J].Annals of Nutrition and Metabolism,2017,71(Suppl.1):17-22.
[24] GONG Y J,YANG H,WANG X,et al.Early intervention with cecal fermentation broth regulates the colonization and development of gut microbiota in broiler chickens[J].Frontiers in Microbiology,2019,10:1422.

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