Effects of Clostridium butyricum on Microbial Community Structure in Jejunum of Weaned Piglets

WANG Yuanxia; ZOU Xiaoting; ZHENG Youxiu; YANG Hua; GONG Yujie; LIU Xiuting; LYU Wentao; XIAO Yingping

doi:10.3969/j.issn.1006-267x.2020.11.018

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 11: 5128 - 5136

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.018

Effects of Clostridium butyricum on Microbial Community Structure in Jejunum of Weaned Piglets

WANG Yuanxia ,
ZOU Xiaoting ,
ZHENG Youxiu ,
YANG Hua ,
GONG Yujie ,
LIU Xiuting ,
LYU Wentao ,
XIAO Yingping

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1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Co-Built by Provincial and Ministry(in Reparation), Hangzhou 310021, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Received date: 2020-03-25

Online published: 2020-11-16

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Abstract

The study was conducted to investigate the effects of Clostridium butyricum on microbial community structure in the jejunum of weaned piglets. A total of 162 (25±1)-day-old healthy Duroc×Landrace×Yorkshire piglets with the body weight of (6.24±0.32) kg were randomly divided into 3 groups with 3 replicates per group and 18 pigs per replicate. Pigs in the control group were fed a basal diet, and those in the experimental groups were fed the basal diet supplemented with 500 (CB500 group) and 2 000 mg/kg (CB2000 group) Clostridium butyricum, respectively. The trail lasted for 30 days. At the end of the experiment, every 6 piglets (2 pigs in each replicate) from each group were slaughtered and used to collect jejunal contents, and the microbial community structure in jejunum was measured by high-throughput sequencing technology and quantitative PCR (qPCR). The results showed that, compared with the control group, the average body weight of weaned piglets in the CB500 group and CB2000 group had an increasing trend, but had no significant differences (P>0.05). The operational taxonomic unit (OTU) number and Shannon index of microbiota in jejunum of weaned piglets in the CB2000 group were significantly higher than those in the control group (P<0.05). At phylum level, Firmicutes, Actinobacteria, Euryarchaeota, Proteobacteria, Cyanobacteria and Bacteroidetes were the dominant phyla in jejunum of weaned piglets. Compared with the control group, the Bacteroidetes relative abundance in both CB500 group and CB2000 group was significantly increased (P<0.05), whereas the Euryarchaeota relative abundance was significantly decreased (P<0.05). At genus level, SMB53, Staphylococcus, Dietzia, Methanobrevibacter, Lactobacillus, Clostridium, Streptococcus, Corynebacterium, Weissella and Roseburia were the dominant genera. Compared with the control group, the relative abundance of opportunistic pathogen such as Pseudomonas and Escherichia was significantly decreased in the Clostridium butyricum groups (P<0.05), while the relative abundance of Coprococcus, Roseburia, Butyricicoccus, Blautia and Ruminococcus was significantly increased (P<0.05). The qPCR analysis showed that the relative abundance of Enterobacteriaceae and Escherichia coli in the CB500 group and CB2000 group was significantly reduced compared with the control group (P<0.05). These results indicate that Clostridium butyricum can increase the bacterial diversity in the jejunum of piglets, and effectively improve the intestinal microbial community structure of piglets by increasing the relative abundance of beneficial bacteria such as Coprococcus, Roseburia, Butyricicoccus and Blautia, but decreasing the relative abundance of pathogens such as Enterobacteriaceae and Pseudomonas.

Key words： Clostridium butyricum; weaned piglets; jejunum; microbial community structure

Cite this article

WANG Yuanxia , ZOU Xiaoting , ZHENG Youxiu , YANG Hua , GONG Yujie , LIU Xiuting , LYU Wentao , XIAO Yingping . Effects of Clostridium butyricum on Microbial Community Structure in Jejunum of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5128 -5136 . DOI: 10.3969/j.issn.1006-267x.2020.11.018

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