Effects of Lactobacillus salivarius SNK-6 on Egg Translucency and Intestinal Flora of Xinyang Black-Feathered Laying Hens

  • HE Xiaoxiao ,
  • CHE Peixin ,
  • WEI Lichun ,
  • LU Kui ,
  • YAN Huaxiang
Expand
  • 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201120, China;
    2. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201120, China;
    3. Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, National Poultry Engineer Research Center, Shanghai 201106, China;
    4. Shanghai Agricultural Development Promotion Center, Shanghai 200335, China

Received date: 2020-04-08

  Online published: 2020-11-16

Abstract

This experiment was conducted to investigate the effects of Lactobacillus salivarius SNK-6 on egg translucency and intestinal flora of Xingyang black-feathered laying hens. Sixty four 45-week-old Xingyang black-feathered laying hens producing translucent egg were divided into 7 groups. Laying hens in control group (16 hens) were fed a basal diet, and the others in 6 experimental group (8 hens per group) were fed experimental diets containing 1.0×106, 5.0×106, 2.5×107, 1.25×108, 6.25×108 and 3.125×109 CFU/hens viable Lactobacillus salivarius SNK-6, respectively. The pretest lasted for 1 week, and the experiment lasted for 4 weeks. Egg translucency were detected every day, and intestinal content samples were collected for detection and analysis of metagenomics after the experiment. The results showed as follows: 1) feeding 2.5×107 CFU/hens Lactobacillus salivarius SNK-6 decreased egg translucency degree, and continuously reduced egg translucent; continued to increasing feeding level of Lactobacillus salivarius SNK-6 could not reduce egg translucent. 2) The metagenomics results showed that there were more intestinal microbial species of laying hens in level of 2.5×107 CFU/hens Lactobacillus salivarius SNK-6, the number of new microbial species in ileum were 31% of that in control group, different species in cecum was 8% of that in control group. The number of Lactobacillus salivarius in ileum in level of 2.5×107 CFU/hens Lactobacillus salivarius SNK-6 was increased by 239%, the number of Chlamydia in ileum was decreased by 50%, and the number of Escherichia coli was reduced by 28%. 3) Gene function in level of 2.5×107 CFU/hens Lactobacillus salivarius SNK-6 was more abundant, the amount of EggNOG intestinal metabolic function annotation was 130% of that in control group, CAZy annotation protease was 139% of that in control group, metabolic process of KEGG annotation was 128% of that in control group, and disease processes was 82% of that in control group. In conclusion, Lactobacillus salivarius SNK-6 can reduce egg translucency, regulate intestinal flora, improve gut metabolism and reduce disease risk.

Cite this article

HE Xiaoxiao , CHE Peixin , WEI Lichun , LU Kui , YAN Huaxiang . Effects of Lactobacillus salivarius SNK-6 on Egg Translucency and Intestinal Flora of Xinyang Black-Feathered Laying Hens[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5230 -5242 . DOI: 10.3969/j.issn.1006-267x.2020.11.028

References

[1] CHOUSALKAR K K,FLYNN P,SUTHERLAND M,et al.Recovery of Salmonella and Escherichia coli from commercial egg shells and effect of translucency on bacterial penetration in eggs[J].International Journal of Food Microbiology,2010,142(1/2):207-213.
[2] 张铭容,叶劲松,张子丽,等.鸡蛋壳暗斑对鸡蛋贮藏性能的影响[J].食品与机械,2016,32(6):118-122.
[3] 张铭容.鸡蛋壳暗斑形成原因及暗斑对鸡蛋贮藏性能影响的研究[D].博士学位论文.雅安:四川农业大学,2016.
[4] 张铭容,叶劲松,李昕阳,等.鸡蛋壳暗斑成因[J].食品与机械,2016,32(5):38-42,90.
[5] WANG D H,LI Y J,LIU L,et al.Traits of eggshells and shell membranes of translucent eggs[J].Poultry Science,2017,96(2):351-358.  
[6] 王德贺.鸡蛋暗斑的形成机理探索研究[D].博士学位论文.北京:中国农业大学,2017.
[7] 霍学婷,耿顺菊,赵国先,等.鸡蛋暗斑随储存时间变化规律及存在部位的探究[J].聊城大学学报(自然科学版),2019,32(4):41-46.
[8] 张名凯,严华祥,邹晓庭,等.影响鸡蛋产生暗斑的因素研究[J].上海农业学报,2019,35(6):72-76.
[9] DEIDDA F,AMORUSO A,NICOLA S,et al.New approach in acne therapy:a specific bacteriocin activity and a targeted anti IL-8 property in just 1 probiotic strain,the L. salivarius LS03[J].Journal of Clinical Gastroenterology,2018,52(Suppl.1):S78-S81.
[10] SORNPLANG P,LEELAVATCHARAMAS V,SOIKUM C.Heterophil phagocytic activity stimulated by Lactobacillus salivarius L61 and L55 supplementation in broilers with Salmonella infection[J].Asian-Australasian Journal of Animal Sciences,2015,28(11):1657-1661.  
[11] MESSAOUDI S,MANAI M,KERGOURLAY G,et al.Lactobacillus salivarius:bacteriocin and probiotic activity[J].Food Microbiology,2013,36(2):296-304.  
[12] O'SHEA E F,O'CONNOR P M,O'SULLIVAN O,et al.Bactofencina,a new type of cationic bacteriocin with unusual immunity[J].mBio,2013,4(6):e00498-13.
[13] PIDUTTI P,FEDERICI F,BRANDI J,et al.Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by the Lactobacillus salivarius SGL 03[J].Journal of Applied Microbiology,2018,124(2):398-407.  
[14] REN D Y,LI C,QIN Y Q,et al.Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests[J].Anaerobe,2015,35:22-27.
[15] SHI D,LV L X,FANG D Q,et al.Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats[J].Scientific Reports,2017,7(1):6927.
[16] LONG S L,GAHAN C G M,JOYCE S A.Interactions between gut bacteria and bile in health and disease[J].Molecular Aspects of Medicine,2017,56:54-65.
[17] SAYAN H,ASSAVACHEEP P,ANGKANAPORN K,et al.Effect of Lactobacillus salivarius on growth performance,diarrhea incidence,fecal bacterial population and intestinal morphology of suckling pigs challenged with F4+ enterotoxigenic Escherichia coli[J].Asian-Australasian Journal of Animal Sciences,2018,31(8):1308-1314.  
[18] SHOKRYAZDAN P,JAHROMI M F,LIANG J B,et al.Effects of a Lactobacillus salivarius mixture on performance,intestinal health and serum lipids of broiler chickens[J].PLoS One,2017,12(5):e0175959.
[19] BLAJMAN J E,OLIVERO C A,FUSARI M L,et al.Impact of lyophilized Lactobacillus salivarius DSPV 001P administration on growth performance,microbial translocation,and gastrointestinal microbiota of broilers reared under low ambient temperature[J].Research in Veterinary Science,2017,114:388-394.
[20] 严华祥,颜瑶,杨长锁,等.鸡回肠唾液乳杆菌影响鸡蛋暗斑形成的研究[C]//中国畜牧兽医学会2018年学术年会禽病学分会第十九次学术研讨会论文集.南宁:中国畜牧兽医学会,2018.
[21] ZHU W H,LOMSADZE A,BORODOVSKY M.Ab initio gene identification in metagenomic sequences.Nucleic Acids Research,2010,38(12):e132.
[22] 严华祥.新杨黑羽蛋鸡配套系[M].北京:中国农业出版社,2019.
[23] 颜瑶.新杨黑羽蛋鸡消化器官生长发育及肠道微生物变化规律研究[D].硕士学位论文.上海:上海海洋大学,2019:32.
Outlines

/