Effects of Heat Stress on Serum Immune and Antioxidative Indexes and Relative Expression of Related Genes in Sheep

  • SHI Lulu ,
  • WANG Zheqi ,
  • XU Yuanqing ,
  • MAO Chenyu ,
  • GUO Shiwei ,
  • JIN Xiao ,
  • SHI Binlin
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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-04-07

  Online published: 2020-11-16

Abstract

This study was conducted to evaluate the effects of heat stress on serum immune and antioxidant indexes and related gene expression of sheep. Eighteen 3-year-old Dorper×Mongolian crossbred ewes were randomly allocated into three groups: control group in natural room temperature (25.23 ℃), preference temperature group furnished with an air-conditioner (19.60 ℃), and high temperature group furnished with a radiator (27.19 ℃). The trial lasted for 28 days. Blood and leukocyte samples were collected on days 14 and 28 to analysis the immune and antioxidant indexes and relative gene expression. The results showed as follows: compared with preference temperature group, 1)on day 14, the content of immunoglobulin G (IgG) in serum in control group and high temperature group was significantly decreased (P<0.05), while the interleukin-2 (IL-2) content in serum and its gene expression in leukocyte were significantly increased (P<0.05), tumor necrosis factor-α (TNF-α) content in serum and its gene expression in leukocyte in high temperature group were significantly increased (P<0.05). On day 28, the contents of immunoglobulin A (IgA), IgG, immunoglobulin M (IgM) in serum in control group and high temperature group were significantly decreased (P<0.05), interleukin-1 (IL-1) content in serum and interleukin-1β(IL-1β) gene expression in leukocyte in high temperature group were significantly increased (P<0.05), while the IL-2 content in serum was significantly decreased (P<0.05). 2) On day 14, catalase (CAT) activity in serum and its gene expression in leukocyte in control group and high temperature group were significantly decreased (P<0.05), while the genes expression of superoxide dismutase 2 (SOD2) and nuclear transcription factor 2 (Nrf2) in leukocyte were significantly increased (P<0.05), total antioxidant capacity (T-AOC) in serum in high temperature group was significantly decreased (P<0.05), while the content of malonaldehyde (MDA) in serum was significantly increased (P<0.05). On day 28, the activity of CAT, glutathion peroxidase (GPx), total superoxide dismutase (T-SOD) and T-AOC in serum in control group and high temperature group were significantly decreased (P<0.05), and the content of MDA in serum in high temperature group was significantly increased (P<0.05). In conclusion, chronic heat stress can reduce antioxidant capacity and suppress the immune system of sheep.

Cite this article

SHI Lulu , WANG Zheqi , XU Yuanqing , MAO Chenyu , GUO Shiwei , JIN Xiao , SHI Binlin . Effects of Heat Stress on Serum Immune and Antioxidative Indexes and Relative Expression of Related Genes in Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5275 -5284 . DOI: 10.3969/j.issn.1006-267x.2020.11.032

References

[1] 彭孝坤.急性冷热应激对肉羊生理生化指标和HSP70家族基因表达的影响[D].硕士学位论文.杨凌:西北农林科技大学,2019.
[2] KIM J M,KIM S S,LEE Y D.Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice[J].NeuroReport,2015,26(13):767-772.  
[3] MANISH K,JINDAL R,NAYYAR S.Influence of heat stress on antioxidant status in Beetal goats[J].Indian Journal of Small Ruminants,2011,17(2):178-181.
[4] ZHANG M Q,JIANG M,BI Y,et al.Autophagy and apoptosis act as partners to induce germ cell death after heat stress in mice[J].PLoS One,2012,7(7):e41412.
[5] CAROPRESE M,ALBENZIO M,BRUNO A,et al.Effects of shade and flaxseed supplementation on the welfare of lactating ewes under high ambient temperatures[J].Small Ruminant Research,2012,102(2/3):177-185.
[6] MARAI I F M,EI-DARAWANY A A,FADIEL A,et al.Physiological traits as affected by heat stress in sheep-a review[J].Small Ruminant Research,2007,71(1/2/3):1-12.
[7] DA COSTA M J R P,DA SILVA R G,DE SOUZA R C.Effect of air temperature and humidity on ingestive behaviour of sheep[J].International Journal of Biometeorology,1992,36(4):218-222.  
[8] LACETERA N.Effect of environment on immune functions[M]//COLLIER R J,COLLIER J L.Environmental physiology of livestock.Chichester (England):Wiley-Blackwell,2012:165-179.
[9] BERNABUCCI U,RONCHI B,LACETERA N,et al.Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season[J].Journal of Dairy Science,2002,85(9):2173-2179.  
[10] DE LA FUENTE M.Effects of antioxidants on immune system ageing[J].European Journal of Clinical Nutrition,2002,56(Suppl.3):S5-S8.
[11] 赵宗胜,米拉古丽,江华,等.冷、热应激对奶牛血液生理生化指标影响[J].中国奶牛,2011(22):18-22.
[12] XU Y Q,WANG Z Q,QIN Z,et al.Effects of chitosan addition on growth performance,diarrhoea,anti-oxidative function and serum immune parameters of weaned piglets[J].South African Society for Animal Science,2018,48(1):142-150.  
[13] TAO S,MONTEIRO A P A,THOMPSON I M,et al.Effect of late-gestation maternal heat stress on growth and immune function of dairy calves[J].Journal of Dairy Science,2012,95(12):7128-7136.  
[14] LACETERA N,BERNABUCCI U,SCALIA D,et al.Lymphocyte functions in dairy cows in hot environment[J].International Journal of Biometeorology,2005,50(2):105-110.  
[15] WEGNER T N,SCHUH J D,NELSON F E,et al.Effect of streess on blood leucocyte and milk somatic cell counts in dairy cows[J].Journal of Dairy Science,1976,59(5):949-956.  
[16] 马燕芬,陈琦,杜瑞平,等.热应激对奶山羊瘤胃上皮细胞屏障通透性的影响[J].中国农业科学,2013,46(21):4478-4485.
[17] STRONG R A,SILVA E B,CHENG H W,et al.Acute brief heat stress in late gestation alters neonatal calf innate immune functions[J].Journal of Dairy Science,2015,98(11):7771-7783.  
[18] LAWRENCE T,FONG C.The resolution of inflammation:anti-inflammatory roles for NF-κB[J].The International Journal of Biochemistry & Cell Biology,2010,42(4):519-523.  
[19] OGANDO D G,PAZ D,CELLA M,et al.The fundamental role of increased production of nitric oxide in lipopolysaccharide-induced embryonic resorption in mice[J].Reproduction,2003,125(1):95-110.  
[20] XIAO W H.Advances in NF-κB signaling transduction and transcription[J].Cellular and Molecular Immunology,2004,1(6):425-435.
[21] JUN C,ZHOU Y X,JIN X D,et al.Expression of interleukin-2 in Candidal balanoposthitis and its clinical significance[J].Chinese Medical Journal,2011,124(17):2776-2778.
[22] ZHAO K,HE W Q,GAO W,et al.Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity[J].Virology Journal,2011,8:562.
[23] SUN Y H,LIU J,YE G P,et al.Protective effects of zymosan on heat stress-induced immunosuppression and apoptosis in dairy cows and peripheral blood mononuclear cells[J].Cell Stress and Chaperones,2018,23(5):1069-1078.  
[24] NAZIFI S,SAEB M,ROWGHANI E,et al.The influences of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T3),thyroxine (T4) and cortisol concentrations[J].Comparative Clinical Pathology,2003,12(3):135-139.  
[25] WIEGERS G J,STEC I E M,STERZER P,et al.Glucocorticoids and the immune response[M]//STECKLER T,KALIN N H,REUL J M H M.Handbook of stress and the brain.Amsterdam:Elsevier,2005.
[26] CARROLL J A,FORSBERG N E.Influence of stress and nutrition on cattle immunity[J].Veterinary Clinics of North America:Food Animal Practice,2007,23(1):105-149.  
[27] 齐晓龙.共轭亚油酸对产蛋鸡抗氧化机能的影响[D].博士学位论文.北京:中国农业科学院,2013:10-15.
[28] GUAN K L,XIONG Y.Regulation of intermediary metabolism by protein acetylation[J].Trends in Biochemical Sciences,2011,36(2):108-116.  
[29] AHMAD H,TIAN J K,WANG J J,et al.Effects of dietary sodium selenite and selenium yeast on antioxidant enzyme activities and oxidative stability of chicken breast meat[J].Journal of Agricultural and Food Chemistry,2012,60(29):7111-7120.  
[30] 王玲,罗宗刚,蔡明成,等.热应激对肉牛血清离子、酶活性、抗氧化及免疫指标的影响[J].西南师范大学学报(自然科学版),2017,42(12):37-41.
[31] WANG R,PAUL V J,LUESCH H.Seaweed extracts and unsaturated fatty acid constituents from the green alga Ulva lactuca as activators of the cytoprotective Nrf2-ARE pathway[J].Free Radical Biology and Medicine,2013,57:141-153.
[32] 李慧.NF-κB通路负调控Keap1-Nrf2通路及其分子机制研究[D].硕士学位论文.天津:天津大学,2009.
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