Effects of Different levels of Double-Low Rapeseed Oil Diets on Rumen Fermentation Characteristics and Methane Production of Yak in Vitro

ZHANG Qunying; HAO Lizhuang; NIU Jianzhang; SUN Lu; DUOJI Ouzhu; LIU Shujie

doi:10.3969/j.issn.1006-267x.2020.11.035

2020 , Vol. 32 >Issue 11: 5302 - 5313

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.035

Effects of Different levels of Double-Low Rapeseed Oil Diets on Rumen Fermentation Characteristics and Methane Production of Yak in Vitro

ZHANG Qunying ,
HAO Lizhuang ,
NIU Jianzhang ,
SUN Lu ,
DUOJI Ouzhu ,
LIU Shujie

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1. Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Academy of Science and Veterinary Medicine of Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Plateau Yak Research Center, Xining 810016, China;
2. Yadong County Pastoral Service Centers of Tibet Autonomous Region Shigatse, Shigatse 857600, China

Received date: 2020-04-16

Online published: 2020-11-16

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Abstract

The purpose of this experiment was to study the effects of different levels of double-low rapeseed oil diets on rumen fermentation characteristics and methane (CH₄) production of yak in vitro, in order to provide the theoretical foundation for the rational use of double-low rapeseed in rumen fermentation. Using a single factor experimental design, the fermentation substrates were 4 groups of different levels of double-low rapeseed oil diets which compound with 4 kinds of concentrate and silage oat grass, and the ratio of concentrate to forage of was 50:50, the double-low rapeseed oil levels in each group were 3.26% (group Ⅰ), 4.58% (group Ⅱ), 5.45% (group Ⅲ) and 6.27% (group Ⅳ), respectively. At 3, 6, 9, 12, 24, 36 and 48 h of in vitro fermentation, the gas production was recorded and the gas production parameters were calculated, the dry matter disappearance rate in vitro (IVDMD) and the CH₄ production, pH and contents of ammonia nitrogen (NH₃-N), microbial protein (MCP) and volatile fatty acid (VFA) in fermentation liquid were determined for 48 h simulating rumen fermentation cultivation in vitro. The results showed as follows: 1) the 48 h gas production and slow-degraded gas production of group Ⅳ were significantly lower than those of other groups (P<0.05), and the theoretical total gas production of group Ⅰ was significantly higher than that of other groups (P<0.05). 2) The contents of acetic acid and total volatile fatty acids (TVFA) in fermentation liquid of group Ⅳ were significantly higher than those of groups Ⅰand Ⅱ (P<0.05), the contents of isobutyric acid and isovaleric acid in fermentation liquid of group Ⅲ were significantly higher than those of other groups (P<0.01), and the fermentation liquid butyric acid content of group Ⅳ was significantly higher than that of other groups (P<0.01). 3) The 24 h IVDMD of group Ⅳ was significantly higher than that of other groups (P<0.05). 4) The fermentation liquid 12 h CH₄ production of group Ⅲ was significantly lower than that of other groups (P<0.01), the fermentation liquid 24 h CH₄ production of group Ⅳ was significantly lower than that of groups Ⅰ and Ⅱ (P<0.01), and the fermentation liquid 48 h CH₄ production of group Ⅳ was significantly lower than that of other groups (P<0.01). 5) There was a significant negative correlation between CH₄ production and dietary ether extract (EE) content (P<0.01), the prediction equation for CH₄ production (y) in vitro using dietary EE content (x) was: y=-0.53x＋13.53 (R²=0.962 7). In conclusion, the increase of oil content different levels in double-low rapeseed oil diets have some effects on the kinetic parameters and dynamic changes of gas production, improve the rumen fermentation and effectively reduce the CH₄ production. When the levels of double-low rapeseed oil is as high as 6.27%, it can inhibit gas production and IVDMD. Dietary EE content can be used as an effective index to predicate CH₄ production of yak in vitro.

Key words： double-low rapeseed oil; fermentation in vitro; yak; methane production

Cite this article

ZHANG Qunying , HAO Lizhuang , NIU Jianzhang , SUN Lu , DUOJI Ouzhu , LIU Shujie . Effects of Different levels of Double-Low Rapeseed Oil Diets on Rumen Fermentation Characteristics and Methane Production of Yak in Vitro[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5302 -5313 . DOI: 10.3969/j.issn.1006-267x.2020.11.035

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