Effects of Dietary Protein Level on Rumen Morphology, Microbial Community Structure and Function of Weaned Lambs

  • LI Haiqin ,
  • JIA Jianlei ,
  • CHEN Qian ,
  • HOU Shengzhen ,
  • GUI Linsheng
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  • College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

Received date: 2020-05-07

  Online published: 2020-11-16

Abstract

This experiment was conducted to study the effects of dietary protein level on rumen morphology, microbial community structure and function of weaned lambs. A total of 120 small tailed Han sheep twin lambs in Haidong area of Qinghai province with a similar body weight of [(3.17±4.52) kg] were selected, lambs were weaned at 50 days of age and randomly divided into 3 groups with 40 lambs per group. Lambs in each group were fed experimental diets with protein levels of 12% (group Ⅰ), 14% (group Ⅱ) and 16% (group Ⅲ), respectively. The experiment lasted for 40 days. The results showed as follows: 1) the average daily gain of group Ⅱ was significantly higher than that of groups Ⅰ and Ⅲ (P<0.05). 2) The rumen papillary width of group Ⅰ was significantly lower than that of groups Ⅱ and Ⅲ (P<0.05), the rumen papillary density and muscular layer thickness of group Ⅱ were significantly higher than those of group Ⅰ (P<0.05), and the rumen stratum corneum thickness of group Ⅰ was significantly higher than that of group Ⅱ (P<0.05). 3) At the family level, the dominant species of each group were Ruminococcaceae, Lachnospiraceae, Christensenellaceae and Veillonellaceae. The relative abundance of Ruminococcaceae of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), the relative abundance of Lachnospiracea of group Ⅲ was significantly higher than that of group Ⅱ (P<0.05), and the relative abundances of Prevotellaceae, Bacteroidales_BS11_gut_group and Rikenellaceae of group Ⅱ were significantly higher than those of groups Ⅰ and Ⅲ (P<0.05). 4) The prediction of 16S rDNA gene function showed that rumen microbial community function of lambs mainly focused on carbohydrate metabolism. In conclusion, when the dietary protein level is 14%, the average daily gain of lambs is the highest, the rumen tissue develops well, the rumen development tends to perfect, the relative abundances of beneficial microbial community are higher, the rumen microflora structure is improve, and it conducive to promote the growth and development of lambs.

Cite this article

LI Haiqin , JIA Jianlei , CHEN Qian , HOU Shengzhen , GUI Linsheng . Effects of Dietary Protein Level on Rumen Morphology, Microbial Community Structure and Function of Weaned Lambs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5331 -5340 . DOI: 10.3969/j.issn.1006-267x.2020.11.038

References

[1] 柴建民.断母乳日龄对羔羊生长性能与胃肠道发育的影响[D].硕士学位论文.北京:中国农业科学院,2015.
[2] NEMATI M,AMANLOU H,KHORVASH M,et al.Rumen fermentation,blood metabolites,and growth performance of calves during transition from liquid to solid feed:effects of dietary level and particle size of al-falfa hay[J].Journal of Dairy Science,2015,98(10):7131-7141.  
[3] 周小乔.粗饲料来源和能量供应对奶牛乳蛋白合成调控的影响[D].博士学位论文.哈尔滨:东北农业大学,2015.
[4] 王芳,徐元君,牛俊丽,等.体外产气法评价反刍动物饲料营养价值的研究[J].中国畜牧兽医,2016,43(1):76-83.
[5] HADDAD S G,NASR R E,MUWALLA M M.Optimum dietary crude protein level for finishing Awassi lambs[J].Small Ruminant Research,2001,39(1):41-46.  
[6] 吕凯,侯生珍,王志有,等.蛋白质水平对早期断奶藏羔羊复胃发育的影响[J].黑龙江畜牧兽医,2013(19):54-56.
[7] 张乃锋,屠焰,刁其玉.幼龄反刍动物健康培育体系构建及其科学问题[J].科学通报,2017,62(26):2999-3007.
[8] 侯生珍,吕凯,王志有,等.蛋白质水平及Lys/Met对早期断奶藏羔羊应激和免疫的影响[J].中国畜牧杂志,2013,49(15):65-69.
[9] 佚名.中国饲料成分及营养价值表(2019年第30版)[J].中国饲料,2019(22):111-116.
[10] EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.  
[11] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QⅡME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.  
[12] LESMEISTER K E,TOZER P R,HEINRICHS A J.Development and analysis of a rumen tissue sampling procedure[J].Journal of Dairy Science,2004,87(5):1336-1344.  
[13] 杨春涛,刁其玉,司丙文,等.挥发性脂肪酸在反刍动物瘤胃上皮吸收转运及调节作用[J].中国畜牧杂志,2015,51(7):78-83.
[14] 汪晓娟,刘婷,李发弟,等.开食料补饲日龄对羔羊瘤胃和小肠组织形态的影响[J].草业学报,2016,25(4):172-178.
[15] SHE N Z,SEYFERT H M,LÖHRKE B,et al.An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats[J].The Journal of Nutrition,2004,134(1):11-17.  
[16] 于洋,格日乐玛,闫素梅,等.放牧与舍饲育肥对绒山羊胃肠道组织形态的影响[J].动物营养学报,2020,32(2):775-783.
[17] LIU J H,XU T T,LIU Y J,et al.A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats[J].American Journal of Physiology Regulatory,Integrative and Comparative Physiology,2013,305(3):R232-R241.
[18] 解彪.不同NDF水平饲粮对羔羊生长性能和瘤胃发育的影响[D].硕士学位论文.晋中:山西农业大学,2018.
[19] 李靖.枯草芽孢杆菌对荷斯坦育成牛生长性能和瘤胃微生物区系的影响[D].硕士学位论文.泰安:山东农业大学,2019.
[20] 张兴夫,杜瑞平,高民.奶牛对饲粮中蛋白质的摄取利用特点及影响因素[J].中国畜牧杂志,2014,50(18):42-47.
[21] 王丹丹.奶牛饲粮与瘤胃纤维和蛋白降解菌多样性的关系[D].硕士学位论文.兰州:甘肃农业大学,2013.
[22] WU S G,WANG G T,ANGERT E R,et al.Composition,diversity,and origin of the bacterial community in grass carp intestine[J].PLoS One,2012,7(2):e30440.
[23] 柏妍,郎侠,王彩莲,等.饲粮中添加牛至精油和莫能菌素对荷斯坦犊牛血清生化指标、消化酶活性及瘤胃微生物区系的影响[J].畜牧兽医学报,2019,50(12):2458-2469.
[24] 张莉.饲粮纤维对仔猪肠道屏障功能的影响及调控机制的研究[D].博士学位论文.北京:中国农业科学院,2018.
[25] 郭凯,唐梦琪,霍倩倩,等.不同蛋白质水平饲粮对犊牛瘤胃微生物区系多样性及组成的影响[J].家畜生态学报,2019,40(11):22-28.
[26] 吴琼,王思珍,张适,等.基于16S rRNA高通量测序技术分析草原红牛瘤胃微生物多样性和功能预测的研究[J].畜牧与兽医,2020,52(1):62-67.
[27] SINGH K M,REDDY B,PATEL D,et al.High potential source for biomass degradation enzyme discovery and environmental aspects revealed through metagenomics of Indian buffalo rumen[J].Biomed Research International,2014,2014:267189.
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