Nutrient Requirement of Isoleucine in Juvenile Tiger Puffer (Takifugu rubripes)

  • SUN Zhiyuan ,
  • WEI Yuliang ,
  • XU Houguo ,
  • SUN Shuguang ,
  • LIANG Mengqing
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  • 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
    2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    3. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

Received date: 2020-03-25

  Online published: 2020-11-16

Abstract

This experiment was conducted to evaluate the effects of dietary isoleucine level on growth performance, protein utilization and free amino acids contents in serum and muscle of juvenile tiger puffer, and discuss the requirement of isoleucine. Six isonitrogenous and isoenergetic diets were formulated to contain graded levels of isoleucine (1.23%, 1.47%, 1.61%, 1.98%, 2.17% and 2.31%, respectively) from dietary ingredients and crystalline isoleucine. Juvenile tiger puffer with an initial body weight of (29.00±0.01) g were fed for 10 weeks, each diet treatment had 3 replicates with 35 fish per replicate. The results indicated that protein productive value (PPV) was first increased and then declined with the increasing of isoleucine levels in diets, 1.98% and 2.17% groups had higher values, and was significantly higher than 1.23% group (P<0.05); body crude protein content was first increased and then declined, 1.61%, 1.98% and 2.17% groups had higher values, and was significantly higher than 1.23% group (P<0.05). Serum free isoleucine contents were increased first and then remained constant as the dietary isoleucine levels increased. The muscle free isoleucine contents was declined first and then increased as the dietary isoleucine levels increased. Special growth rate (SGR), feed efficiency (FE), condition factor (CF), hepatosomatic index (HSI) and viscerosomatic index (VSI) of tiger puffer were not significantly affected by dietary isoleucine levels (P>0.05). Based on the quadratic regression analysis of the PPV, body crude protein content and muscle free isoleucine content, the optimum requirement of juvenile tiger puffer (Takifugu rubripes) for isoleucine is estimated to be in the range of 1.83% to 1.96%.

Cite this article

SUN Zhiyuan , WEI Yuliang , XU Houguo , SUN Shuguang , LIANG Mengqing . Nutrient Requirement of Isoleucine in Juvenile Tiger Puffer (Takifugu rubripes)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5347 -5356 . DOI: 10.3969/j.issn.1006-267x.2020.11.040

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