Dietary Protein Requirement of Pelophylax nigromaculatus Tadpoles in Metamorphosis Stage

WANG Yuzhu; LI Siming; LI Debing; LIU Wenyu; LIU Wenshu; GUO Xiaoze

doi:10.3969/j.issn.1006-267x.2020.11.041

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 11: 5357 - 5364

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.041

Dietary Protein Requirement of Pelophylax nigromaculatus Tadpoles in Metamorphosis Stage

WANG Yuzhu ,
LI Siming ,
LI Debing ,
LIU Wenyu ,
LIU Wenshu ,
GUO Xiaoze

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1. College of Animal Science, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Animal Sciences, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
3. The Aquaculture Farm of Ji'an, Jiangxi Province, Ji'an 343000, China

Received date: 2020-03-24

Online published: 2020-11-16

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Abstract

The purpose of this experiment was to study the effects of dietary protein level on growth indexes, feed utilization, digestive enzyme activities, amino acid metabolic enzyme activities and antioxidant ability of Pelophylax nigromaculatus tadpoles in metamorphosis stage, so as to preliminarily explore the appropriate dietary protein level. A total of 450 Pelophylax nigromaculatus tadpoles at 30 stages of development with an average body weight of 0.43 g were selected. The tadpoles were randomly divided into 5 groups with 3 replicates in each group and 30 tadpoles in each replicate. Five iso-lipidemic and iso-energetic experimental diets with protein levels of 25%, 30%, 35%, 40% and 45% were fed to the 5 groups until the end of stage 46 (complete absorption of the tail). The results showed as follows: 1) the final body weight, weight gain rate (WGR), specific growth rate, feed efficiency (FE) of 40% and 45% protein level groups were significantly higher than those of other groups (P<0.05), and the protein efficiency ratio of 25% protein level group was significantly higher than that of other groups (P<0.05). The regression analysis of WGR and FE showed that the WGR got the highest when dietary protein level was 42.21%, and the FE got the highest when dietary protein level was 41.81%. 2) The intestinal amylase activity of 25% protein level group was significantly higher than that of other groups (P<0.05), and the intestinal trypsin activity of 40% protein level group was significantly higher than that of 25%, 30% and 35% protein level groups (P<0.05). 3) The activities of alanine aminotransferase in liver and adenylate deaminase in muscle of 45% protein level group were significantly higher than those of other groups (P<0.05). 4) The liver catalase activity of 40% protein level group was significantly higher than that of other groups (P<0.05), the liver total antioxidant capacity of 35% protein level group was significantly higher than that of other groups (P<0.05), and the liver malondialdehyde content of 35% protein level group was significantly lower than that of other groups (P<0.05). In conclusion, under the experimental condition, dietary protein levels have effects on growth indices, feed utilization, digestive enzyme activities, amino acid metabolic enzyme activities and antioxidant ability of Pelophylax nigromaculatus tadpoles in metamorphosis stage, and the appropriate dietary protein level is 41.81% to 42.21%.

Key words： Pelophylax nigromaculatus; tadpoles; diet; protein

Cite this article

WANG Yuzhu , LI Siming , LI Debing , LIU Wenyu , LIU Wenshu , GUO Xiaoze . Dietary Protein Requirement of Pelophylax nigromaculatus Tadpoles in Metamorphosis Stage[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5357 -5364 . DOI: 10.3969/j.issn.1006-267x.2020.11.041

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