Based on Erythropoietin Gene Expression and Blood Physiological and Biochemical Indexes to Explore the Adaptability of Hu Sheep in High-Altitude Area of Qinghai

ZHANG Chunmei; CHEN Qian; XIE Wen; REN Hao; ZHANG Ziqi; JIA Jianlei

doi:10.3969/j.issn.1006-267x.2020.11.044

2020 , Vol. 32 >Issue 11: 5380 - 5387

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.044

Based on Erythropoietin Gene Expression and Blood Physiological and Biochemical Indexes to Explore the Adaptability of Hu Sheep in High-Altitude Area of Qinghai

ZHANG Chunmei ,
CHEN Qian ,
XIE Wen ,
REN Hao ,
ZHANG Ziqi ,
JIA Jianlei

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1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China;
2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2020-04-24

Online published: 2020-11-16

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Abstract

The purpose of this experiment was to study the adaptive changes of erythropoietin (EPO) gene expression and blood physiological and biochemical indexes in the blood of Hu sheep after entering high-altitude area in Qinghai. Ninety 8-month-old Hu sheep reserve ewes with 8-month-old and similar body weight [(32.11±1.86) kg] in mid-altitude area (Gansu province, 1 500 m) and high-altitude area (Qinghai province, 3 200 m) were selected, respectively. The Hu sheep in that two places were randomly divided into 3 groups with 30 sheep in each group, respectively. The EPO gene expression and blood physiological and biochemical indexes of Hu sheep in different altitude areas were measured. The result showed that the relative expression level of EPO gene in blood of Hu sheep in the high-altitude area was higher than that of Hu sheep in the mid-altitude area (P<0.05); the blood red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT) and mean corpuscular volume (MCV) of Hu sheep in the high-altitude area were respectively increased by 36.23%, 45.94%, 58.34% and 23.65% (P<0.05), and the white blood cell count (WBC), mean erythrocyte hemoglobin concentration (MCHC), platelet count (PLT) and mean platelet volume (MPV) were respectively reduced by 27.33%, 11.68%, 18.27% and 6.20% (P<0.05) compared with the mid-altitude area. The activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and the content of glucose (Glu) of Hu sheep in the high-altitude area were respectively increased by 10.71%, 16.80% and 62.72% (P<0.05), and the serum total cholesterol (TC) content was reduced by 33.22% compared with the middle-altitude area (P<0.05); the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and the content of malondialdehyde (MDA) in serum of Hu sheep in the high-altitude area were 5.99, 2.35, 4.10, 1.49 times higher than those of Hu sheep in the mid-altitude area (P<0.05), respectively. In summary, Hu sheep raised in the high-altitude area of Qinghai show adaptive changes in the blood EPO gene expression, blood physiological and biochemical indexes, indicating that Hu sheep have the potential to adapt to high-altitude environment.

Key words： Hu sheep; high-altitude area; EPO gene; blood physiological and biochemical indexes; adaptation

Cite this article

ZHANG Chunmei , CHEN Qian , XIE Wen , REN Hao , ZHANG Ziqi , JIA Jianlei . Based on Erythropoietin Gene Expression and Blood Physiological and Biochemical Indexes to Explore the Adaptability of Hu Sheep in High-Altitude Area of Qinghai[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5380 -5387 . DOI: 10.3969/j.issn.1006-267x.2020.11.044

References

[1] 魏国桢,应建翔,江斌,等.湖羊在福建省清流县的适应性观察[J].黑龙江畜牧兽医,2014(12):46-47.
[2] 杨磊,马进忠,王志明,等.湖羊在临夏干旱山区适应性观测[J].畜牧兽医杂志,2019,38(1):81-82,85.
[3] 谢云龙.湖羊在天祝县的适应性及其杂交组合研究[C]//2017年全国养羊生产与学术研讨会暨养羊学分会第七次全国会员代表大会论文集.石家庄:中国畜牧兽医学会养羊学分会,2017:66.
[4] 王则夫.牦牛和藏羚羊高海拔适应机制的趋同进化研究[D].硕士学位论文.兰州:兰州大学,2016.
[5] MALLET R T,RYOU M G.Erythropoietin:endogenous protection of ischemic brain[J].Vitamins and Hormones,2017,105:197-232.
[6] HUANG Y C,HSU C C,WANG J S.High-intensity interval training improves erythrocyte osmotic deformability[J].Medicine and Science Sports and Exercise,2019,51(7):1404-1412.

[7] 杨秀平,肖向红,李大鹏.动物生理学[M].3版.北京:高等教育出版社,2012:342-350.
[8] 邹思湘.动物生物化学[M].5版.北京:中国农业出版社,2012:126-127.
[9] 卢晓丽,赵彦玲,吴征王,等.西藏色瓦藏绵羊高原适应性的血液生理学特性研究[J].西南农业学报,2019,32(6):1443-1447.
[10] 张桢.阿司匹林对抗血小板促进肿瘤增殖作用的初步研究[D].硕士学位论文.西安:西北大学,2019.
[11] 赵士杰.不同海拔地区外周血细胞和血小板参数变化及意义[J].青海医药杂志,2013,43(2):51-52.
[12] 王烽.四种SPF猪群体血液生理生化及遗传特征分析[D].硕士学位论文.哈尔滨:东北农业大学,2019.
[13] 郭丹丹,董矜,郭晓东,等.不同海拔高原适应相关肝脏功能与脂代谢变化[J].现代生物医学进展,2013,13(1):71-74.
[14] 王志敏,徐亚欧,杨磊,等.不同海拔藏鸡生理生化指标的研究[J].畜牧与饲料科学,2018,39(2):1-7.
[15] WU J,STEFANIAK J,HAFNER C,et al.Intermittent hypoxia causes inflammation and injury to human adult cardiac myocytes[J].Anesthesia and Analgesia,2016,122(2):373-380.

[16] 辛笛.低氧环境对大林姬鼠生活节律及血液生理生化指标的影响[D].硕士学位论文.牡丹江:牡丹江师范学院,2017.
[17] ZHAO H T,WANG Z Y,CHENG C L,et al.UV protective effect of anthocyanin extract from Lonicera caerulea var.edulis[J].Applied Mechanics and Materials,2012,195-196:1294-1299.
[18] GHISELLI A,SERAFINI M,NATELLA F,et al.Total antioxidant capacity as a tool to assess redox status:critical view and experimental data[J].Free Radical Biology and Medicine,2000,29(11):1106-1114.

[19] 姜宁,杨坤,张爱忠,等.谷胱甘肽对育肥羊机体抗氧化能力的影响[J].饲料工业,2015,36(23):45-48.
[20] FANG J,MENON M,KAPELLE W,et al.EPO modulation of cell-cycle regulatory genes,and cell division,in primary bone marrow erythroblasts[J].Blood,2007,110(7):2361-2370.

[21] 许海霞.高海拔和低海拔部分家畜促红细胞生成素(EPO)基因遗传多态性研究[D].硕士毕业论文.兰州:甘肃农业大学,2012.
[22] 包鹏甲,裴杰,阎萍,等.藏羊EPO基因遗传多样性与高原低氧适应性[J].江苏农业学报,2014,30(3):581-585.
[23] 吴周林,赵莉,周贵凤,等.九龙牦牛EPO基因部分片段遗传多态性分析[J].当代畜牧,2017(24):23-25.
[24] 谢静.甘肃鼢鼠(Myospalax cansus)EPO基因克隆及其mRNA在脑组织的表达研究[D].硕士学位论文.西安:陕西师范大学,2014.
[25] 张湑泽,郭松长,都玉蓉.高原鼢鼠低氧适应研究进展[J].生命科学研究,2015,19(6):554-558.

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