Influence Factors and Variation in Determination of Phytase Activity in Commercial Phytase and Diet Phytase

WU Yuxuan; SU Yanfang; ZHAO Feng; ZHANG Hu; XU Erhua; ZHAO Wei; LI Zhefeng; HE Xi

doi:10.3969/j.issn.1006-267x.2020.11.046

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 11: 5397 - 5405

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.046

Influence Factors and Variation in Determination of Phytase Activity in Commercial Phytase and Diet Phytase

WU Yuxuan ,
SU Yanfang ,
ZHAO Feng ,
ZHANG Hu ,
XU Erhua ,
ZHAO Wei ,
LI Zhefeng ,
HE Xi

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1. College of Animal Science, Hunan Agricultural University, Changsha 410128, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Provincial Key Agricultural Enterprise Research Institute of Encapsulated Feed Additive, Hangzhou King Techina Feed Co., Ltd., Hangzhou 311107, China

Received date: 2020-03-27

Online published: 2020-11-16

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Abstract

The objective of this experiment was to study the influence factors and variation in determination of phytase activity in commercial phytase and diet phytase. Four trials were included in this experiment. Trial 1 was to investigate the influence of the dilution buffer category and the centrifugal speed for the reaction solution after the color reaction on the determined value of phytase activity with the national standard method. Dilution solutions of buffer 1 (acetic acid/sodium acetate buffer) or buffer 2 (acetic acid/sodium acetate+triton X-100+BSA), and centrifugal speeds for the reaction solution of 3 000, 5 000 or 8 000 r/min for the reaction solution were used in a 2×3 factorial arrangement in a completely randominze design. Trial 2 was to investigate the influence of dilution rate of phytase on the determined value of activity. The dilution rates were 4 treatments of 150, 200, 250 or 300 times for commercial phytase and 3 treatments of 5, 10 or 15 times for diet phytase. Trial 3 was to examine the interference of feed ingredients on the determined activity of phytase. A one-sample comparison design was adopted to each of corn, soybean meal, wheat bran, rapeseed meal, or cottonseed meal with the phytase added as 4 g per kilogram of feed ingredient. Trial 4 was to investigate the variation of phytase activity determined in commercial phytase and diet phytase in 14 days by a single-factor completely random design. The results showed as follows: 1) the determined phytase activities in diet were not different at centrifugal speed of 3 000, 5 000 or 8 000 r/min when the buffer 1 was used for the diluent solution (P>0.05). But the determined phytase activities of diets were increased by the centrifugal speed when the buffer 2 was used for the diluent solution (P<0.05). 2) With increment of the dilution rate, the determined activities of commercial phytase firstly increased and then decreased (P<0.05), however, the determined phytase activity in diets decreased linearly (P<0.05). 3) In feed ingredients added phytase, the determined value of total phytase activity in corn was significantly lower than the calculated value (P<0.05). But greater determined than calculated total phytase activities were observed in soybean meal, wheat bran, rapeseed meal and cottonseed meal (P<0.05). 4) The intra-day, inter-day and total coefficient of variation (CV) of the determined phytase activity in the commercial phytase were 3.22%, 6.29% and 6.88%. The intra-day, inter-day and total CV of the determined phytase activity in the diet supplemented with phytase were 1.55%, 3.83% and 4.06%. In conclusion, acetic acid/sodium acetate buffer is more suitable to use as a diluent for determination of phytase activity. The determined value of phytase activity in diet is affected by the centrifugation speed and dilution rate, but the determined value of commercial phytase activity is only affected by the dilution rate. Feed ingredients significantly interfere with the determination of phytase activity. The variation in the determined phytase activity is lower in the diet than that in commercial phytase.

Key words： phytase; influence factors; variation

Cite this article

WU Yuxuan , SU Yanfang , ZHAO Feng , ZHANG Hu , XU Erhua , ZHAO Wei , LI Zhefeng , HE Xi . Influence Factors and Variation in Determination of Phytase Activity in Commercial Phytase and Diet Phytase[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5397 -5405 . DOI: 10.3969/j.issn.1006-267x.2020.11.046

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