Effects of Electrical Stimulation Frequencies on Antioxidant Capacity and Expression of Antioxidant-Related Genes in Factor-Erythroid 2-Related Factor 2/Antioxidant Reaction Sequence Element Signaling Pathway in Broiler Myoblasts

  • XU Lei ,
  • ZHANG Xin ,
  • HUANG Huayun ,
  • LI Ning ,
  • WAN Xiaoli ,
  • YANG Haiming ,
  • WANG Zhiyue
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  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Institute of Poultry Science, Yangzhou 225125, China

Received date: 2020-04-18

  Online published: 2020-11-16

Abstract

This experiment was to investigate the effects of different electrical stimulation frequencies on the antioxidant capacity and expression of antioxidant-related genes in factor-erythroid 2-related factor 2 (Nrf2)/antioxidant reaction sequence element (ARE) signaling pathway in broiler myoblasts. The myoblasts were divided into the control group and five electrical stimulation groups, with three replicates in each group. The myoblasts in control group were not treated with electrical stimulation. The myoblasts in the other groups were respectively stimulated with 50, 160, 320, 640 and 1 280 Hz electricity combined with a stable voltage of 30 V and a stimulation period of 10 s. Cells were cultured for 30 min after the treatments. Then the changes of antioxidant capacity and expression of antioxidant-related genes in Nrf2/ARE signaling pathway were measured in broiler myoblasts. The results showed that different electrical frequencies had no significant effect on the degree of cell injury (P>0.05). The hydrogen peroxide (H2O2) content in 320 Hz group was significantly lower than that in 50, 640 and 1 280 Hz groups (P<0.05). The reduced glutathione (GSH)content and superoxide dismutase (SOD) activity in 320 Hz group were significantly higher than those in the control group and 640 Hz group (P<0.05). The mRNA relative expression levels of Nrf2, Kelch-like ECH-associated protein 1 (Keap1) and NAD(P)H quinone oxidoreductase l (NQO1) were significantly higher in 320 Hz group than those in other electrical stimulation groups (P<0.05). The mRNA relative expression of glutathione-S-transferase T1 (GSTT1) was the highest in 320 Hz group as compared with other groups (P<0.05). In conclusion, a stimulating frequency at 320 Hz can improve the antioxidant capacity and the expression of antioxidant-related genes in Nrf2/ARE signaling pathway without changing cell morphology or causing obvious damage to cell membrane in broiler myoblasts.

Cite this article

XU Lei , ZHANG Xin , HUANG Huayun , LI Ning , WAN Xiaoli , YANG Haiming , WANG Zhiyue . Effects of Electrical Stimulation Frequencies on Antioxidant Capacity and Expression of Antioxidant-Related Genes in Factor-Erythroid 2-Related Factor 2/Antioxidant Reaction Sequence Element Signaling Pathway in Broiler Myoblasts[J]. Chinese Journal of Animal Nutrition, 2020 , 32(11) : 5415 -5423 . DOI: 10.3969/j.issn.1006-267x.2020.11.048

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