A high performance liquid chromatography method was developed for the determination of carvacrol and thymol contents in feed by matrix solid-phase dispersion technology under fluorescence detector. With water and n-hexane used as extraction solvent, the carvacrol and thymol in the extraction solution were extracted by the multiple adsorption material and eluted by acetonitrile. Analysis was performed on an Eclipse plus C18 column (250 mm×4.6 mm, 5 m) with the mobile phase consisting of acetonitrile/water (65 : 35, V/V) at a flow rate of 1.0 mL/min. The excitation wavelength of the fluorescence detector was 274 nm, and the emission wavelength was 304 nm. Different feed samples were analyzed by the established methods. Good linearity was obtained in the ranges of 1 to 500 ng/mL, with linear coefficients (R2) higher than 0.999 0. The limit of detection (LOD) was 0.6 μg/kg and the quantification limit (LOQ) was 2.0 μg/kg. The recoveries for carvacrol and thymol spiked (2, 10 and 20 μg/kg) in feeds ranged from 80.2% to 108.6% and the relative standard deviations (RSD) were lower than 10.2%. The proposed method is reliable, fast, and easy to perform, making it applicable for routine analysis to provide technical support for market regulation.
WANG Shi
,
HU Shen
,
LOU Yingxia
,
XIAO Zhiming
,
TIAN Jing
,
LI Yang
,
FAN Xia
. Determination of Carvacrol and Thymol Contets in Feeds by Liquid Chromatography-Fluorescence Combined with Matrix Solid-Phase Dispersion Technology[J]. Chinese Journal of Animal Nutrition, 2020
, 32(11)
: 5472
-5481
.
DOI: 10.3969/j.issn.1006-267x.2020.11.054
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