Effects of Bacillus velezensis on Growth Performance,Fecal Microbiota and Metabolites in Pigs

LIU Shaona; ZHANG Bin; XIANG Decai; ZHAO Zhiyong; CHANG Yajie; SHA Qian; ZHAO Yanguang

doi:10.3969/j.issn.1006-267x.2020.12.016

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 12: 5622 - 5635

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.016

Effects of Bacillus velezensis on Growth Performance,Fecal Microbiota and Metabolites in Pigs

LIU Shaona ,
ZHANG Bin ,
XIANG Decai ,
ZHAO Zhiyong ,
CHANG Yajie ,
SHA Qian ,
ZHAO Yanguang

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Yunnan Animal Science and Veterinary Institute, Kunming 650224, China

Received date: 2020-05-21

Online published: 2020-12-07

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Abstract

The study aimed to explore the effects of the Bacillus velezensis on the growth performance, fecal microbiota and metabolites in pigs. Twenty hybrid combinations pigs with an average body weight of (34.00±0.25) kg were randomly divided into 2 groups with 2 replicates in each group and 5 pigs in each replicate. The pigs in control group were fed a basal diet, and the pigs in experimental group were fed the basal diet with Bacillus velezensis (10⁷ CFU/kg), respectively. The pre-experimental period was 7 days, and the experimental period was 21 days. The results showed as follows: 1) there was no significant difference between two groups in feed/gain (F/G) and average daily feed intake (ADFI) (P>0.05); compared with control group, the average daily gain (ADG) in experimental group was significantly increased (P<0.05). 2) The alpha diversity of 2 groups had no significant difference (P>0.05). Compared with control group, adding Bacillus velezensis could affect the relative abundances of fecal bacteria, at the phylum level, the Firmicutes/Bacteroidetes was significantly decreased (P<0.05); at the genus level, the relative abundance of Ruminococcus_1 (P<0.01), unclassified_f_Lachnospiraceae (P<0.01), Lactobacillus (P<0.05) and Prevotella_9 (P<0.05) were significantly increased (P<0.05), while that of the Streptococcus and Ruminococcaceae_UCG-002 were significantly decreased (P<0.05). The results which was analyzed by LEfSe showed that the relative abundance of bacterial communities between two groups were significantly different (P<0.05). When the LDA value was higher than 2.0, 56 biomarkers were obtained, while 20 biomarkers were due to experimental group. The largest LDA value of bacteria in control group was Selenomonadales, and that in experimental group was Ruminococcaceae_UCG-014, which were the bacteria with the greatest difference in relative abundance. 3) Partial least squares discrimination analysis (PLS-DA) analysis showed that there was significant difference in positive and negative model (P<0.05). The metabolite analysis showed that compared with the control group, the relative contents of pelargonidin 3-sophoroside and nigellic acid were up-regulated significantly in experimental group (P<0.01), and those of sedanonic acid, methyl-10-hydroperoxy-8E, 12Z, 15Z-octadecatrienoate, oxindole and oxyquinoline were all down-regulated significantly (P<0.05). In summary, the addition of Bacillus velezensis can optimize the structure of fecal microbiota in pigs, improve the relative abundance of Lactobacillus, the relative abundance of unclassified_f_Lachnospiraceae and Ruminococcus_1 which can produce short-chain fatty acid, reduce the relative abundance of Streptococcus which is the opportunistic pathogen, and improve the relative content of pelargonidin 3-sophoroside. It has the potential function of growth-promoting and reducing disease.

Key words： pig; Bacillus velezensis; fecal microbiota; microbial diversity; metabolite

Cite this article

LIU Shaona , ZHANG Bin , XIANG Decai , ZHAO Zhiyong , CHANG Yajie , SHA Qian , ZHAO Yanguang . Effects of Bacillus velezensis on Growth Performance,Fecal Microbiota and Metabolites in Pigs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5622 -5635 . DOI: 10.3969/j.issn.1006-267x.2020.12.016

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