Effects of Icariin on Performance, Egg quality, Redox State and Gene Expression of Inflammatory Cytokines in Oviduct of Laying Hens

  • TONG Xiaofeng ,
  • ZHANG Lei ,
  • HUANG Jie ,
  • LYU Ping ,
  • ZHOU Zhongxin
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  • 1. College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Jianli Wenshi Livestock Co., Ltd., Jingzhou 434000, China

Received date: 2020-05-03

  Online published: 2020-12-07

Abstract

The aim of this study was to investigate the effects of dietary icariin on performance, egg quality, redox state, and gene expression of inflammatory cytokines in oviduct of laying hens. Two hundred and sixteen 54-week-old health Lohmann pink-shell laying hens with similar performance were randomly divided into 3 groups with 6 replicates per group and 12 hens per replicate. Hens in control group were fed a basal diet, and the others in experimental groupsⅠand Ⅱ were fed the basal diets supplemented with 0.05% and 0.20% icariin (as fresh basis), respectively. The pretest lasted for 1 week and the experiment lasted for 12 weeks. The results showed as follows; compared with control group, 1) average egg weight of laying hens in experimental group Ⅱ was significantly increased (P<0.05); 2) Albumen height in experimental group Ⅱ was significantly increased at 66 weeks of age (P<0.05); 3) The content of malondialdehyde in oviduct of laying hens in experimental group Ⅱ was significantly decreased (P<0.05); 4) The mRNA relative expression levels of interferon-γ (IFN-γ), interleukin-4 (IL-4) and interleukin-6 (IL-6) in oviduct of laying hens in experimental groups Ⅰ and Ⅱ were significantly decreased (P<0.05), the mRNA relative expression level of interleukin-10 (IL-10) in oviduct in experimental group Ⅰ was significantly decreased (P<0.05), and the mRNA relative expression level of tumor necrosis factor-α (TNF-α) in oviduct in experimental group Ⅱ was significantly decreased (P<0.05). It is concluded that supplemented with 0.20% icariin in diets of laying hens can increase average egg weight and albumen height, reduce oxidative stress of oviduct, and improve immune function of oviduct.

Cite this article

TONG Xiaofeng , ZHANG Lei , HUANG Jie , LYU Ping , ZHOU Zhongxin . Effects of Icariin on Performance, Egg quality, Redox State and Gene Expression of Inflammatory Cytokines in Oviduct of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5676 -5683 . DOI: 10.3969/j.issn.1006-267x.2020.12.021

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