Effects of Supplemental Feeding of Cysteamine and Chromium Nicotinate on Lactation Performance, Antioxidant Performance, Rumen Fermentation Parameters and Microbial Diversity of Buffalo in Summer

ZHANG Huihui; LI Mengwei; TANG Zhenhua; LIANG Xin; PENG Lijuan; PENG Kaiping; WANG Xinfeng; YANG Chengjian

doi:10.3969/j.issn.1006-267x.2020.12.030

2020 , Vol. 32 >Issue 12: 5760 - 5777

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.030

Effects of Supplemental Feeding of Cysteamine and Chromium Nicotinate on Lactation Performance, Antioxidant Performance, Rumen Fermentation Parameters and Microbial Diversity of Buffalo in Summer

ZHANG Huihui ,
LI Mengwei ,
TANG Zhenhua ,
LIANG Xin ,
PENG Lijuan ,
PENG Kaiping ,
WANG Xinfeng ,
YANG Chengjian

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1. Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs(Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China;
2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China

Received date: 2020-05-12

Online published: 2020-12-07

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Abstract

The purpose of this experiment was to study the effects of supplemental feeding of cysteamine and chromium nicotinate lactation performance, antioxidant performance, rumen fermentation parameters and microbial diversity of buffalo in summer. A single-factor randomized block design was used in this experiment. Twenty healthy Nile-Rafi milk buffaloes with similar body weight [(615±21) kg], parity (3 to 5 parities) and milk yield (5 to 6 kg/d) were selected and divided into 4 groups (groups Ⅰ, Ⅱ, Ⅲ and Ⅳ) with 5 buffaloes in each group. Group Ⅰ was the control group, and buffaloes in the group Ⅰ were fed a basal diet. Groups Ⅱ, Ⅲ and Ⅳ were test groups, and buffaloes in the groups Ⅱ, Ⅲ and Ⅳ were fed the basal diet+22.5 g/(d·head) cysteamine, the basal diet+24 mg/(d·head) chromium (chromium nicotinate form, the same below) and the basic diet+22.5 g/(d·head) cysteamine+24 mg/(d·head) chromium, respectively. The experiment was carried from June to August of 2019. The pre-feeding period was 1 week and the test period was 4 weeks. The results showed as follows: 1) supplemental feeding of chromium nicotinate and cysteamine had no significant effects on the surface temperature and respiratory rate of buffalo (P>0.05). Compared with groupⅠ, the rectal temperature of group Ⅳ decreased significantly (P<0.05). 2) The dry matter intake of group Ⅰ was significantly lower than that of groups Ⅱ and Ⅳ (P<0.05). The apparent digestibility of crude protein and neutral detergent fiber of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 3) The milk yield of group Ⅳ was significantly higher than that of groups Ⅰ, Ⅱ and Ⅲ (P<0.05). The yield of 4% fat corrected milk (FCM) of groups Ⅲ and Ⅳ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05). The milk protein rate of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅳ (P<0.05). The milk total solid content of group Ⅲ was significantly higher than that of other groups (P<0.05). 4) The content of acetate in rumen fluid of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ (P<0.05). The contents of propionate, isobutyrate, total volatile fatty acids and microbial protein in rumen fluid of groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05). The differences of butyrate content, acetate/propionate, ammonia nitrogen content, pH and isovalerate content in each group were not significant (P>0.05). 5) The contents of C12:0, C14:0, C16:0, C18:2 (cis-9, trans-11), and C20:3n3 in the milk of group Ⅳ were significantly lower than those of group Ⅰ (P<0.05); the content of C16:0 in the milk of groups Ⅲ and Ⅳ was significantly lower than that of group Ⅰ (P<0.05). 6) There were no significant differences in serum catalase, total superoxide dismutase and glutathione peroxidase activities among groups (P>0.05); the content of serum malondialdehyde of groups Ⅱ and Ⅳ was significantly lower than that of group Ⅰ (P<0.05), and the serum total antioxidant capacity was significantly lower than that of group Ⅳ (P<0.05). The content of serum growth hormone of groups Ⅱ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05); the content of serum estradiol of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05). The content of serum thyroxine of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05); the activity of serum aspartate aminotransferase of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). 7) Supplemental feeding of chromium nicotinate and cysteamine could significantly increase the number of bacteria in rumen fluid (P<0.05); the number of methanogen in rumen fluid of group Ⅱ was significantly higher than that of group Ⅰ (P<0.05). The relative abundance of Bacteroidetes in rumen fluid of groups Ⅱ and Ⅲ was higher than that in group Ⅰ, while the relative abundance of Firmicutes was lower than that in group Ⅰ. In summary, the simultaneous feeding of cysteamine and chromium nicotinate in summer can increase the dry matter intake of buffaloes, and improve the apparent digestibility of nutrients, lactation performance, rumen fermentation parameters and microbial composition, and thus alleviate the adverse effects of heat stress on the body of buffaloes.

Key words： chromium nicotinate; cysteamine; lactation performance; antioxidant performance; microbial diversity

Cite this article

ZHANG Huihui , LI Mengwei , TANG Zhenhua , LIANG Xin , PENG Lijuan , PENG Kaiping , WANG Xinfeng , YANG Chengjian . Effects of Supplemental Feeding of Cysteamine and Chromium Nicotinate on Lactation Performance, Antioxidant Performance, Rumen Fermentation Parameters and Microbial Diversity of Buffalo in Summer[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5760 -5777 . DOI: 10.3969/j.issn.1006-267x.2020.12.030

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