Molecular Identification Method of Raw Fish Species Composition in Fish Meal

ZHOU Meng; ZHAN Yanhang; LIU Zhenyu; LIANG Rishen; TANG Yimei; GAO Hongyan; HUANG Yanhua; LIN Li

doi:10.3969/j.issn.1006-267x.2020.12.033

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 12: 5795 - 5804

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.033

Molecular Identification Method of Raw Fish Species Composition in Fish Meal

ZHOU Meng ,
ZHAN Yanhang ,
LIU Zhenyu ,
LIANG Rishen ,
TANG Yimei ,
GAO Hongyan ,
HUANG Yanhua ,
LIN Li

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1. Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
3. Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China;
4. Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, China

Received date: 2020-05-31

Online published: 2020-12-07

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Abstract

This study was to develop a sensitive, accurate and simple molecular method to identify the raw fish species composition of fish meal. Two pairs of primers, cytb and 16S rDNA were used to amplify the genomic DNA of 9 species of marine and fresh water fish, and 7 kinds of commercial fish meal, respectively. It was found that the amplification efficiency of cytb primer were superior to that of 16S rDNA, clear and bright target bands appeared for each sample. The PCR products of 7 fish meal amplified by cytb primer were ligated to pMD 19-T vector, and transformed into Escherichia coli DH5α competent cells, the identified positive clones were sequenced and analyzed using BlastN. The results showed that 33 fish species belonging to genera of Engraulis, Decapterus, Sardina, Trachurus, Brevoortia and Tilapia et al. were identified in 7 commercial fish meal samples. The number of fish species was far beyond that labeled on the fish meal products. To verify the reliability of the above method, two samples of self-formulated fish meal with known supplemented fish species were identified with the same methods. The results were consistent with those of the design. In conclusion, the molecular identification method based on cytb primer can be used to identify the raw fish species composition in fish meal, and the results will provide abundant information for accurate judgment of fish meal quality.

Key words： fish meal; raw fish; cytb primer; molecular identification

Cite this article

ZHOU Meng , ZHAN Yanhang , LIU Zhenyu , LIANG Rishen , TANG Yimei , GAO Hongyan , HUANG Yanhua , LIN Li . Molecular Identification Method of Raw Fish Species Composition in Fish Meal[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5795 -5804 . DOI: 10.3969/j.issn.1006-267x.2020.12.033

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