Contour Identification and Analysis of Bile Acids in Bile and Plasma of Scophthalmus maximus L.

YAO Ting; WEI Xian; XUE Min; SUN Baozhong; GU Xu; ZHANG Songshan

doi:10.3969/j.issn.1006-267x.2020.12.035

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 12: 5816 - 5826

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.035

Contour Identification and Analysis of Bile Acids in Bile and Plasma of Scophthalmus maximus L.

YAO Ting ,
WEI Xian ,
XUE Min ,
SUN Baozhong ,
GU Xu ,
ZHANG Songshan

Expand

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100032, China;
2. Beijing Institute of Feed Control, Beijing 100061, China;
3. Laboratory of Quality & Safety Risk Assessment for Animal Products of China Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2020-05-27

Online published: 2020-12-07

Fold

Abstract

In this study, the bile acids (BAs) in bile and plasma of Scophthalmus maximus L.,were analyzed which focused on the species and contents of BAs, in order to support technical support and data basis for relevant studies of BAs in fish. In this study, ultra-liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was utilized for qualitative and quantitative analysis of 30 kinds of BAs in bile and plasma with the assistant of internal standard. The method could match the requirements of BAs analysis in biological samples with good sensitivity, accuracy and precision. The results showed as follows: 6 kinds of primary BAs and 4 kinds of secondary BAs were detected in bile, among which the primary BAs were taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), cholic acid (CA), glycochenodeoxycholic acid (GCDCA), glycocholic acid (GCA) and chenodeoxycholic acid (CDCA), arranged in order respectively. The secondary BAs were arranged in order of content level, namely, tauro-beta-muricholic acid (TβMCA), tauro-omega-muricholic acid (TωMCA), glycolithocholic acid (GLCA) and glycohyocholic acid (GHCA). In plasma, low contents of 5 primary BAs and 3 secondary BAs were discovered. The primary BAs were CA, CDCA, GCDCA, TCA and TCDCA, and the secondary BAs were murine cholic acids (ωMCA) and TωMCA. In this study, murine cholic acids are found in fish for the first time. This study, which can enrich the fish BAs spectrum library, can be a reference method for the contour identification and analysis of other fish BAs, and provide the data basis for subsequent studies on BAs.

Key words： bile acids; bile; plasma; Scophthalmus maximus L.; UPLC-MS/MS

Cite this article

YAO Ting , WEI Xian , XUE Min , SUN Baozhong , GU Xu , ZHANG Songshan . Contour Identification and Analysis of Bile Acids in Bile and Plasma of Scophthalmus maximus L.[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5816 -5826 . DOI: 10.3969/j.issn.1006-267x.2020.12.035

References

[1] WAHLSTRÖM A,SAYIN S I,MARSCHALL H U,et al.Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism[J].Cell Metabolism,2016,24(1):41-50.

[2] MONTE M J,MARIN J J G,ANTELO A,etal.Bile acids:chemistry,physiology,and pathophysiology[J].World Journal of Gastroenrerology,2009,15(7):804-816.

[3] VITEK L,HALUZIK M.The role of bile acids in metabolic regulation[J].The Journal of Endocrinology,2016,228(3):R85-R96.
[4] SCHMID A,SCHLEGEL J,THOMALLA M,et al.Evidence of functional bile acid signaling pathways in adipocytes[J].Molecular and Cellular Endocrinology,2019,483:1-10.
[5] PRINZ P,HOFMANN T,AHNIS A,et al.Plasma bile acids show a positive correlation with body mass index and are negatively associated with cognitive restraint of eating in obese patients[J].Frontiers in Neuroscience,2015,9:199.
[6] SONNE D P,VAN NIEROP F S,KULIK W,et al.Postprandial plasma concentrations of individual bile acids and FGF-19 in patients with type 2 diabetes[J].Journal of Clininal Endocrinology and Metabolism,2016,101(8):3002-3009.

[7] ALBAUGH V L,FLYNN C R,CAI S,et al.Early increases in bile acids post Roux-en-Y gastric bypass are driven by insulin-sensitizing,secondary bile acids[J].Journalof Clininal Endocrinology and Metabolism,2015,100(9):E1225-E1233.
[8] 苏冠宁,孙赫,吴高峰,等.胆汁酸的肠肝循环及胆汁酸受体在糖脂代谢中的调控作用[J].动物营养学报,2019,31(8):3511-3521.
[9] DE AGUIAR VALLIM T Q,TARLING E J,EDWARDS P A.Pleiotropic roles of bile acids in metabolism[J].Cell Metabolism,2013,17(5):657-669.

[10] HOU Y B,YAO L,YAO L,et al.Effects of chromium yeast,tributyrin and bile acid on growth performance,digestion and metabolism of Channa argus[J].Aquaculture Research,2018,50(3):836-846.
[11] JIANG M,WEN H,GOU G W,et al.Preliminary study to evaluate the effects of dietary bile acids on growth performance and lipid metabolism of juvenile genetically improved farmed tilapia (Oreochromis niloticus) fed plant ingredient-based diets[J].AquacultureNutrition,2018,24(4):1175-1183.
[12] 曾本和,廖增艳,向枭,等.胆汁酸对草鱼(Ctenopharyngodon idellus)的生长性能、肌肉营养成分及消化酶活性的影响[J].渔业科学进展,2017,38(2):99-106.
[13] YU HH,ZHANG L L,CHEN P,et al.Dietary bile acids enhance growth,and alleviate hepatic fibrosis induced by a high starch diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 pathway in Micropterus salmoides[J].Frontiers in Physiology,2019,10:1430.
[14] 张晶,李文祥,邹红,等.草鱼不同组织胆汁酸组成分析[J].水生生物学报,2017,41(2):479-482.
[15] HAGEY L R,MØLLERP R,HOFMANN A F,et al.Diversity of bile salts in fish and amphibians:evolution of a complex biochemical pathway[J].Physiological and Biochemical Zoology,2010,83(2):308-321.

[16] DUTTA M,CAI J W,GUI W,et al.A review of analytical platforms for accurate bile acid measurement[J].Analytical and Bioanalytical Chemistry,2019,411(19):4541-4549.

[17] 黄炳山,李宝山,张利民,等.胆汁酸对大菱鲆幼鱼生长、脂肪代谢酶及血清生化的影响[J].上海海洋大学学报,2015,24(5):737-744.
[18] GU Y Y,WANG X K,LI J H,et al.Analyses of gut microbiota and plasma bile acids enable stratification of patients for antidiabetic treatment[J].Nature Communications,2017,8:1785.
[19] ALNOUTI Y.Bile acid sulfation:a pathway of bile acidelimination and detoxification[J].Toxicological Sciences,2009,108(2):225-246.

[20] 斯日古楞,刘晓玲,李冬梅,等.超高效液相色谱快速分析不同哺乳动物胆汁中胆汁酸[J].分析化学,2015,43(5):675-681.
[21] 王一博,王春雨,曲范娜,等.高效液相色谱-电雾式检测器同时测定猪、牛、羊、熊胆粉中5种胆汁酸的含量[J].分析化学研究简报,2014,41(1):109-112.
[22] 王朋,林森,吴德,等.胆汁酸的营养生理作用及代谢调控研究进展[J].动物营养学报,2019,31(5):2002-2011.
[23] 刘雅楠,雷凯,向东,等.HPLC-MS/MS法同时测定大鼠血浆中26种胆汁酸[J].药物分析杂志,2018,38(10):1767-1774.
[24] JUN H,YANG L,WANG R X,et al.Metabolic profiling of bile acids in human and mouse blood by LC-MS/MS in combination with phospholipid-depletion solid-phase extraction[J].Analytical Chemistry,2015,87(2):1127-1136.

[25] 沈淑娇,张志荣,曾金,等.LC-MS/MS测定小鼠肝脏中10种胆汁酸浓度的方法与应用[J].肝脏,2015,20(4):285-290,294.
[26] TSAI S J J,ZHONG Y S,WENG J F,et al.Determination of bile acids in pig liver,pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms[J].Journal of Chromatography A,2011,1218(3):524-533.

[27] CHIANG J Y L,FERRELL J M.Bile acid metabolism in liver pathobiology[J].Gene Expression,2018,18(2):71-87.

[28] TAKAHASHI S,UKAMI T,MASUO Y,et al.Cyp2c70 is responsible for the species difference in bile acid metabolism between mice and humans[J].Journal of Lipid Research,2016,57(12):2130-2137.

[29] JIA W,XIE G X,JIA W P.Bile acid-microbiota crosstalk in gastrointestinal inflammation and carcinogenesis[J].Nature Reviews Gastroenterology & Hepatology,2018,15(2):111-128.

[30] CUI Y,XU B,ZHANGX Q,et al.Diagnostic and therapeutic profiles of serum bile acids in women with intrahepatic cholestasis of pregnancy-a pseudo-targeted metabolomics study[J].Clinica Chimica Acta,2018,483:135-141.
[31] ZHANG Y,CHEN P,LIANG X F,et al.Metabolic disorder induces fatty liver in Japanese seabass,Lateolabrax japonicas fed a full plant protein diet and regulated by cAMP-JNK/NF-kB-caspase signal pathway[J].Fish & Shellfish Immunology,2019,90:223-224.
[32] HOFMANN A F.The continuing importance of bile acids in liver and intestinal disease[J].Archives of Internal Medicine,1999,159(22):2647-2658.

[33] DE PAIVAM J N,MENEZESH C,DA SILVAJ C C, et al.New method for the determination of bile acids in human plasma by liquid-phase microextraction using liquid chromatography-ion-trap-time-of-flight mass spectrometry[J].Journal of Chromatography A,2015,1388:102-109.
[34] 杜震宇.养殖鱼类脂肪肝成因及相关思考[J].水产学报,2014,38(9):1628-1638.
[35] LIAN G X F,HU L,DONG Y C,et al.Substitution of fish meal by fermented soybean meal affects the growth performance and flesh quality of Japanese seabass (Lateolabrax japonicus)[J].Animal Feed Science and Technology,2017,229:1-12.
[36] 韩建华,苏薇,崔巍,等.超高效液相色谱-串联质谱联用检测血清胆汁酸谱方法的建立及初步临床应用[J].中华检验医学杂志,2012,35(3):253-256.
[37] VINCENT R P,OMAR S,GHOZLAN S,et al.Higher circulating bile acid concentrations in obese patients with type 2 diabetes[J].Annals of Clinical Biochemistry,2013,50(4):360-364.

[38] WEWALKA M,PATTI M E,BARBATO C,et al.Fasting serum taurine-conjugated bile acids are elevated in type 2 diabetes and do not change with intensification of insulin[J].The Journal of Clinical Endocrinology & Metabolism,2014,99(4):1442-1451.

[39] FERSLEW B C,XIE G X,JOHNSTON C K,et al.Altered bile acid metabolome in patients with nonalcoholic steatohepatitis[J].Digestive Diseases and Science,2015,60(11):3318-3328.

[40] JAHNEL J,ZÖHRER E,ALISI A,et al.Serum bile acid levels in children with nonalcoholic fatty liver disease[J].Journal of Pediatric Gastroenterology and Nutrition,2015,61(1):85-90.
[41] PURI P,DAITA K,JOYCE A,et al.The presence and severity of nonalcoholic steatohepatitis is associated with specific changes in circulating bile acids[J].Hepatology,2018,67(2):534-548.

[42] JIAO N,BAKER S S,CHAPA-RODRIGUEZ A,et al.Suppressed hepatic bile acid signalling despite elevated production of primary and secondary bile acids in NAFLD[J].Gut,2018,67(10):1881-1891.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References