Influences of Dietary Eucommia Bark and Leaf on Growth, Muscle Quality and Collagen Related Genes Expression in Grass Carp (Ctenopharyngodon idellus)

  • YANG Hang ,
  • XU Zhen ,
  • TAN Sumei ,
  • LI Xiaoqin ,
  • CHENG Zhuo ,
  • LENG Xiangjun
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  • 1. National Demonstration Center for Experimental Fisheries Science Education, College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China;
    2. Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN) of the Ministry of Agriculture, College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China;
    3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China

Received date: 2020-05-11

  Online published: 2020-12-07

Abstract

The study investigated the effects of dietary Eucommia ulmoides on growth, muscle quality and collagen related genes expression of grass carp (Ctenopharyngodon idella). A total of 162 grass carp with average body weight of (59.7±0.3) g were selected and divided into 3 groups with 3 replicates in each group and 18 grass carp in each replicate. Control group was fed a basal diet, and the other groups were fed the basal diet with 20 g/kg Eucommia bark (EB) and 40 g/kg Eucommia leaf (EL), respectively. The feeding period was 60 days. The results showed as follows: the weight gain rate of EB and EL groups were 293.4% and 300.0%, and were significantly increased by 3.38% and 5.71% compared with control group (P<0.05), and the feed conversion ratio were significantly decreased by 0.06 and 0.10 (P<0.05). Compared with the control group, dietary EB and EL significantly increased the contents of calcium, total collagen and heat-insoluble collagen in muscle (P<0.05), and significantly decreased muscle lipid content (P<0.05), and the total amino acids content and essential amino acid index in muscle also significantly increased (P<0.05). In addition, the serum glucose, total cholesterol and triglycerides contents of the two groups were lower than those of the control group (P<0.05). In collagen related genes expression, the EB and EL supplementation significantly promoted the relative transcript levels of Ⅰ type collagen α1 (COL1A1), Ⅰ type collagen α2 (COL1A2) and lysyl oxidase (LOX) genes in muscle (P<0.05), while the relative transcript levels of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9)genes significantly decreased (P<0.05). Moreover, dietary EL also significantly increased the relative transcript level of prolyl hydroxylase (PHD) gene (P<0.05). In conclusion, dietary EB and EL improve the growth performance, decrease muscle lipid deposition of grass carp, and increase collagen content in muscle by up-regulating the expression of collagen synthesis-related genes and down-regulating the expression of collagen degradation-related genes.

Cite this article

YANG Hang , XU Zhen , TAN Sumei , LI Xiaoqin , CHENG Zhuo , LENG Xiangjun . Influences of Dietary Eucommia Bark and Leaf on Growth, Muscle Quality and Collagen Related Genes Expression in Grass Carp (Ctenopharyngodon idellus)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5827 -5838 . DOI: 10.3969/j.issn.1006-267x.2020.12.036

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