Effects of Chitosan Oligosaccharide on Digestive Enzyme Activities, Intestinal Microflora Structure, Serum Non-Specific Immune Indices and Disease Resistance of Procambarus clarkii

ZHANG Shiyu; XIE Mengqi; XU Lili; WU Zhixin; TIAN Ding; YUAN Junfa; CHEN Xiaoxuan

doi:10.3969/j.issn.1006-267x.2020.12.039

2020 , Vol. 32 >Issue 12: 5864 - 5874

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.039

Effects of Chitosan Oligosaccharide on Digestive Enzyme Activities, Intestinal Microflora Structure, Serum Non-Specific Immune Indices and Disease Resistance of Procambarus clarkii

ZHANG Shiyu ,
XIE Mengqi ,
XU Lili ,
WU Zhixin ,
TIAN Ding ,
YUAN Junfa ,
CHEN Xiaoxuan

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1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
2. Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan 430070, China;
3. National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2020-05-21

Online published: 2020-12-07

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Abstract

This study was conducted to investigate the effects of dietary chitosan oligosaccharide (COS) on digestive enzyme activities, intestinal microflora structure, serum non-specific immune indices and disease resistance of Procambarus clarkii. A total of 240 Procambarus clarkii with an initial body weight of (17.39±1.20) g were selected and randomly divided into 4 groups with 3 replicates per group and 20 crayfish per replicate. Crayfish in the 4 groups were feed diets supplemented with 0 (C0 group), 200 (C200 group), 400 (C400 group) and 800 mg/kg (C800 group) chitosan oligosaccharide, respectively. The experiment lasted for 14 days. The results showed as follows: 1) the intestinal amylase activity of C200 group and C800 group was significantly higher than that of C0 group (P<0.05), the intestinal lipase and trypsin activities and hepatopancreas amylase and lipase activities of C200 group, C400 group and C800 group were significantly higher than those of C0 group (P<0.05), and the hepatopancreas trypsin activity of C200 group and C400 group was significantly higher than that of C0 group (P<0.05). 2) The Simpson index, Chao1 index, ACE index and Shannon index of C200 group were significantly higher than those of C0 group (P<0.05). Compared with C0 group, the relative abundance of Proteobacteria of C200 group, C400 group and C800 group was decreased, while the relative abundances of Bacteroidetes, Firmicutes and Tenericutes were increased; the Rhodobacter, Lactococcus, Shewanella and Bacteroides had higher relative abundances and common bacterial genus in all the 4 groups. 3) The serum alkaline phosphatase (AKP) activity of C400 group and C800 group was significantly higher than that of C0 group (P<0.05), the serum acid phosphatase (ACP) activity of C400 group was significantly higher than that of C0 group (P<0.05), and the serum catalase (CAT) activity of C200 group, C400 group and C800 group was significantly lower than that of C0 group (P<0.05). 4) At day 7 of white spot syndrome virus (WSSV) challenge, the cumulative mortality rate of Procambarus clarkii was decreased with the dietary chitosan oligosaccharide supplemental level increased. Compared with C0 group, the cumulative mortality rate of C400 group and C800 group was decreased by 42.10% and 57.89%, respectively. In conclusion, dietary adding appropriate amount of chitosan oligosaccharide can improve the digestive enzyme activities, improve the intestinal microflora structure and enhance the resistance to WSSV infection of Procambarus clarkii.

Key words： chitosan oligosaccharide; Procambarus clarkii; digestive enzyme; intestinal microflora; disease resistance

Cite this article

ZHANG Shiyu , XIE Mengqi , XU Lili , WU Zhixin , TIAN Ding , YUAN Junfa , CHEN Xiaoxuan . Effects of Chitosan Oligosaccharide on Digestive Enzyme Activities, Intestinal Microflora Structure, Serum Non-Specific Immune Indices and Disease Resistance of Procambarus clarkii[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5864 -5874 . DOI: 10.3969/j.issn.1006-267x.2020.12.039

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