Effects of Different Copper Sources on Performance, Serum Biochemical Indexes and Liver Related Gene Expression of Raccoon Dogs in Winter Fur-Growing Period

ZHANG Xinyu; ZHANG Haihua; LIU Hanlu; ZHONG Wei; HAN Feifei; WANG Jing; MU Linlin; CHEN Shuangshuang; LI Guangyu

doi:10.3969/j.issn.1006-267x.2020.12.041

2020 , Vol. 32 >Issue 12: 5884 - 5892

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.041

Effects of Different Copper Sources on Performance, Serum Biochemical Indexes and Liver Related Gene Expression of Raccoon Dogs in Winter Fur-Growing Period

ZHANG Xinyu ,
ZHANG Haihua ,
LIU Hanlu ,
ZHONG Wei ,
HAN Feifei ,
WANG Jing ,
MU Linlin ,
CHEN Shuangshuang ,
LI Guangyu

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1. State Key Laboratory of Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
2. Hebei Key Laboratory of Specialty Animal Germplasm Resources Innovation and Utilization, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China

Received date: 2020-05-07

Online published: 2020-12-07

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Abstract

This experiment was conducted to study the effects of different copper sources on performance, organ indexes, serum biochemical indexes and liver related genes expression of raccoon dogs in winter fur-growing period. Sixty healthy male raccoon dogs at the age of (135±5) days with the similar body weight were randomly divided into 4 groups with 15 replicates each and 1 raccoon dog per replicate. The raccoon dogs in the 4 groups were fed the basal diet supplemented with 60 mg/kg copper from copper sulfate (group Ⅰ), basic copper chloride (group Ⅱ) and mixed copper (group Ⅲ, the ratio of basic copper chloride to copper methionine was 1:1) and copper methionine (group Ⅳ). The pre-test period lasted for 7 days and the trial period lasted for 60 days.Eight animals in each group were selected for blood collection and slaughter test. The results showed as follows: 1) the body weight in group Ⅰ was significantly lower than that in other groups (P<0.05), the guard hair long in the group Ⅳ was significantly longer than that in the groups Ⅰ and Ⅱ (P<0.05). 2) There was no significant difference in organ indexes among groups (P>0.05). 3) The serum glucose (GLU) content in groups Ⅲ and Ⅳ was significantly higher than that in the groups Ⅰ and Ⅱ (P<0.05). The serum ceruloplasmin (CER) content in the groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that in the group Ⅰ (P<0.05). The serum total superoxide dismutase (T-SOD) activity in groups Ⅲ and Ⅳ was significantly higher than that in the group Ⅰ (P<0.05). The serum copper and zinc superoxide dismutase (Cu-Zn SOD) activity in group Ⅳ was significantly higher than that in the group Ⅰ (P<0.05) 4) The relative expression level of CER gene in group Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of growth hormone receptor (GHR) gene in groups Ⅲ and Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of insulin-like growth factor-Ⅰ (IGF-Ⅰ) gene in groups Ⅲ and Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of Cu-Zn SOD gene in groups Ⅲ and Ⅳ was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05). Under the condition of this experiment, copper methionine and mixed copper groups significantly increase the body weight, serum CER and Cu-Zn SOD activities, and significantly increase the relative expression levels of GHR, IGF-Ⅰ, CER and Cu-Zn SOD genes. Combined with actual production, it is suggested that organic copper and inorganic copper should be mixed in production.

Key words： copper; raccoon dog; SOD; CER

Cite this article

ZHANG Xinyu , ZHANG Haihua , LIU Hanlu , ZHONG Wei , HAN Feifei , WANG Jing , MU Linlin , CHEN Shuangshuang , LI Guangyu . Effects of Different Copper Sources on Performance, Serum Biochemical Indexes and Liver Related Gene Expression of Raccoon Dogs in Winter Fur-Growing Period[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5884 -5892 . DOI: 10.3969/j.issn.1006-267x.2020.12.041

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