Effects of Dandelion Polysaccharide on Antioxidant Enzyme Activity and Related Gene Expression in Mice

  • KANG Wenjin ,
  • XU Xingjun ,
  • LIU Jiaren ,
  • ZHANG Weiwei ,
  • SHAO Shuli
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  • 1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161006, China;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161007, China

Received date: 2020-05-22

  Online published: 2020-12-07

Abstract

To investigate the effects of dandelion polysaccharide on antioxidant enzyme activity and related gene expression in mice, 48 specific pathogen free (SPF) ICR 5-week-old male mice were randomly divided into control group, high concentration dandelion polysaccharide group [600 mg/(kg·d)], medium concentration dandelion polysaccharide group [300 mg/(kg·d)] and low concentration dandelion polysaccharide group [150 mg/(kg·d)]. Dandelion polysaccharide group was given different concentrations of dandelion polysaccharide once a day by regular gavage, while the control group was given the same amount of normal saline for 4 weeks. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) and malonaldehyde (MDA) content were determined in the serum and liver tissues of mice, and the relative mRNA expression levels of copper and zinc superoxide dismutase (Cu-Zn SOD), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase-1 (GPX-1) and glutathione peroxidase-4 (GPX-4) in liver of mice were determined by qRT-PCR. The results showed that after gavaged with dandelion polysaccharide, the activities of GSH-Px, SOD and T-AOC in mice serum and liver increased significantly (P<0.05), the content of MDA reduced significantly (P<0.05), and the mRNA relative expression levels of Cu-Zn SOD, Mn-SOD, GPX-1 and GPX-4 were up-regulated significantly (P<0.05). Dandelion flavonoids can significantly improve the antioxidant capacity of mice and have anti-aging effects.

Cite this article

KANG Wenjin , XU Xingjun , LIU Jiaren , ZHANG Weiwei , SHAO Shuli . Effects of Dandelion Polysaccharide on Antioxidant Enzyme Activity and Related Gene Expression in Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5910 -5915 . DOI: 10.3969/j.issn.1006-267x.2020.12.044

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