Effects of Different Combinations of Exogenous Enzymes on in Vitro Nutrient Digestibility and Enzymatic Hydrolyzate Gross Energy of Palm Kernel Meal Ingredients

ZHANG Shunfen; ZHONG Ruqing; ZHANG Bifeng; LI Tao; SUN Shudong; CHEN Liang; ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2020.12.045

2020 , Vol. 32 >Issue 12: 5916 - 5924

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.045

Effects of Different Combinations of Exogenous Enzymes on in Vitro Nutrient Digestibility and Enzymatic Hydrolyzate Gross Energy of Palm Kernel Meal Ingredients

ZHANG Shunfen ,
ZHONG Ruqing ,
ZHANG Bifeng ,
LI Tao ,
SUN Shudong ,
CHEN Liang ,
ZHANG Hongfu

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Hunan United-Hope Biotechnology Company, Changsha 410007, China

Received date: 2020-05-25

Online published: 2020-12-07

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Abstract

This experiment was conducted to investigate the effects of different combinations of exogenous enzymes on in vitro dry matter digestibility (IVDMD), in vitro crude protein digestibility (IVCPD), in vitro gross energy digestibility (IVGED) and enzymatic hydrolyzate gross energy (EHGE) of palm kernel meal ingredients using a computer-controlled simulated digestion system (SDS-Ⅱ), and to evaluate the nutritional value of palm kernel meal from two sources [Malaysia palm kernel cake (MPK) and Philippines palm kernel cake (PPK)] and screen the corresponding optimal exogenous enzyme combination. In experiment 1, a 2×3 factor design was adopted, consisted of 6 groups, in which the control groups were MPK and PPK ingredients without exogenous enzymes addition, and the experimental groups were supplemented exogenous enzyme package-A (EP-A), exogenous enzyme package-B (EP-B) in PPK ingredient and supplemented EP-A, EP-B in MPK ingredient, respectively. Using SDS-Ⅱ to simulate the total tract digestive process of pigs, the IVDMD, IVCPD, IVGED and EHGE of each groups were determined. In experiment 2, a single factor completely random design adopted, consisted of 5 groups (EP0, EP1, EP2, EP3 and EP4 groups), in which the control group (EP0 group) were without exogenous enzymes addition, and the experimental groups were supplemented different combinations of exogenous enzymes in MPK ingredient. The IVDMD, IVCPD, IVGED and EHGE of each groups were determined. Five replicates per sample and one digestive tract for each replicate were set in both experiments. The results showed as follows: 1) the IVDMD, IVCPD and IVGED of PPK were significantly higher than those of MPK (P<0.05), but the EHGE of PPK had no significant difference with that of MPK (P>0.05). 2) The supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVGED and EHGE of MPK and PPK (P<0.05), the supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVCPD of MPK (P<0.05), and the supplementation of exogenous enzyme combination EP-B significanltly increased the IVDMD of MPK and PPK (P<0.05). 3) The supplementation of different combinations of exogenous enzymes significanltly increased the IVDMD, IVCPD, IVGED and EHGE of MPK (P<0.05), and the IVDMD, IVGED and EHGE of EP4 group were significantly higher than those of EP1, EP2 and EP3 groups (P<0.05). In conclusion, the IVDMD, IVCPD and IVGED of PPK are higher than those of MPK, which is a better source of palm kernel meal; the supplementation of exogenous enzyme combinations EP-A and EP-B can increase the IVDMD, IVCPD, IVGED and EHGE of palm kernel meal from two sources of which EP-B has better effects; the exogenous enzyme combination of EP4 group is the optimal combination for MPK enzymolysis.

Key words： in vitro method; palm kernel meal; exogenous enzyme combination; EHGE

Cite this article

ZHANG Shunfen , ZHONG Ruqing , ZHANG Bifeng , LI Tao , SUN Shudong , CHEN Liang , ZHANG Hongfu . Effects of Different Combinations of Exogenous Enzymes on in Vitro Nutrient Digestibility and Enzymatic Hydrolyzate Gross Energy of Palm Kernel Meal Ingredients[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5916 -5924 . DOI: 10.3969/j.issn.1006-267x.2020.12.045

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