Immunomodulatory Effects of W/O/W Codonopsis pillosula Polysaccharide Nanoemulsion on Immunosuppressed Mice

SHI Feifei; WANG Dada; CAO Jinhua; YU Shenglan

doi:10.3969/j.issn.1006-267x.2020.12.046

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 12: 5925 - 5931

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.046

Immunomodulatory Effects of W/O/W Codonopsis pillosula Polysaccharide Nanoemulsion on Immunosuppressed Mice

SHI Feifei ,
WANG Dada ,
CAO Jinhua ,
YU Shenglan

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College of Veterinary Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

Received date: 2020-05-20

Online published: 2020-12-07

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Abstract

This experiment was conducted to investigate the immunoregulation effects of W/O/W Codonopsis pillosula polysaccharide nanoemulsion (CPPS-NE) on immunosuppressed mice. A total of 320 healthy mice were dividied into 4 units, and each unit had 80 mice. The 80 mice in each unit were randomly divided into a blank control group, a model control group, high-, medium- and low-dose CPPS-NE groups, high-, medium- and low-dose Codonopsis pillosula polysaccharide solution(CPPS) groups, and each group had 10 mice (half male and half femal). The blank control group and the model control group were given 0.2 mL normal saline by intragastric administration. The high-, medium- and low-dose CPPS-NE groups were gavaged 0.3, 0.2 and 0.1 mL of 900, 600 and 300 mg/kg BW CPPS-NE, respectively, while the high-, medium- and low-dose CPPS groups were gavaged 0.3, 0.2 and 0.1 mL of 900, 600 and 300 mg/kg BW CPPS, respectively. All mice were given with normal saline, CPPS-NE or CPPS by intragastric administration for 14 days, once a day. In addition to the blank control group, other mice were subcutaneously injected with hydrocortisone to obtain immunosuppressive model. The unit 1 detected carbon clearance index, the unit 2 deteced thymus and spleen indexes, the unit 3 detected serum hemolysin content, and the unit 4 detected serum cytokines contents, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10) and interleukin-12 (IL-12) in mice. The results showed that, compared with the model control group, CPPS-NE could significantly improve the immune organ index (P<0.05), phagocytic ability of monocyte macrophages (P<0.05), effectively promote hemolysin synthesis (P<0.05), and significantly increase the contents of TNF-α, IFN-γ, IL-2, IL-6, IL-10 and IL-12 in serum (P<0.05), which was dose-dependent. Moreover, the effect of CPPS-NE was significantly stronger than that of CPPS in the same dose group. The results show that Codonopsis pillosula polysaccharide has immunomodulatory effects on nonspecific immunity, humoral immunity and cellular immunity of immunosuppressed mice, and CPPS-NE can significantly enhance the effect.

Key words： Codonopsis pillosula polysaccharide; nanoemulsion; immunosuppression; immunoregulation

Cite this article

SHI Feifei , WANG Dada , CAO Jinhua , YU Shenglan . Immunomodulatory Effects of W/O/W Codonopsis pillosula Polysaccharide Nanoemulsion on Immunosuppressed Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5925 -5931 . DOI: 10.3969/j.issn.1006-267x.2020.12.046

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