Study on Intestinal Microbial Culturomics of Piglets Before and After Weaning

DONG Bo; WANG Zhilin; WEI Wenkang; JING Xiaohuan; LIU Shi; YAN Qiulong; JIANG Zongyong; LIU Wenhua; HU Yiyin; CHEN Zhuang

doi:10.3969/j.issn.1006-267x.2021.01.019

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 1: 175 - 189

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.019

Study on Intestinal Microbial Culturomics of Piglets Before and After Weaning

DONG Bo ,
WANG Zhilin ,
WEI Wenkang ,
JING Xiaohuan ,
LIU Shi ,
YAN Qiulong ,
JIANG Zongyong ,
LIU Wenhua ,
HU Yiyin ,
CHEN Zhuang

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1. Guangdong Key Laboratory of Crop Germplasm Resources Conservation and Utilization, Agro-Genes Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Guangzhou 510640, China;
3. Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology-Maoming Sub-Center, Maoming 525000, China;
4. China National GeneBank, Shenzhen 510083, China;
5. Department of Microbiology, Dalian Medical University, Dalian 116044, China;
6. Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2020-06-13

Online published: 2021-01-18

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Abstract

In recent years, researchers at home and abroad have proved that the composition of porcine intestinal microorganisms and their metabolite activity play an important role in host health through next-generation sequencing and isolation culture techniques combined with metagenomics related bioinformatics methods. Due to the limitations of traditional culture methods, most of the bacteria in the intestinal tract of pigs still cannot be cultured. Therefore, in order to break through the limitations of studying host-strain interaction and the development and application of probiotics, this study applied high-throughput culturomics to study the microorganisms in ileum and colon contents of piglets before and after weaning combined with aerobic and anaerobic conditions, different culture time and 25 different medium, then a total of 1 385 anaerobic, aerobic and facultative strains were screened, and for all these full-length 16S rRNA sequencing identification and strain preservation were carried out. A total of 5 phyla, 29 genera and 86 species were identified, including some strains which were difficult to cultivate using traditional culture techniques, such as Fusobacteria, Bacteroides, etc., and some probiotic bacteria with potential application value such as Lactobacillus was isolated; more importantly, they contained 116 suspected new strains. The strain information obtained in this study can further improve the database of intestinal microbial species of pigs, lay a foundation for subsequent studies on host-strain interaction, and is of great significance for the development and research of probiotics related products for pig feeding.

Key words： culturomics; piglet weaned; intestine; strains; identification

Cite this article

DONG Bo , WANG Zhilin , WEI Wenkang , JING Xiaohuan , LIU Shi , YAN Qiulong , JIANG Zongyong , LIU Wenhua , HU Yiyin , CHEN Zhuang . Study on Intestinal Microbial Culturomics of Piglets Before and After Weaning[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 175 -189 . DOI: 10.3969/j.issn.1006-267x.2021.01.019

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